Caspase-7 (CASP7) (NM_033340) Human Untagged Clone

CAT#: SC109027

CASP7 (untagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Caspase-7
• Synonyms: CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033340, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGATGATCAGGGCTGTATTGAAGAGCAGGGGGTTGAGGATTCAGCAAATGAAGATTCAGTGGATG
  CTAAGCCAGACCGGTCCTCGTTTGTACCGTCCCTCTTCAGTAAGAAGAAGAAAAATGTCACCATGCGATC
  CATCAAGACCACCCGGGACCGAGTGCCTACATATCAGTACAACATGAATTTTGAAAAGCTGGGCAAATGC
  ATCATAATAAACAACAAGAACTTTGATAAAGTGACAGGTATGGGCGTTCGAAACGGAACAGACAAAGATG
  CCGAGGCGCTCTTCAAGTGCTTCCGAAGCCTGGGTTTTGACGTGATTGTCTATAATGACTGCTCTTGTGC
  CAAGATGCAAGATCTGCTTAAAAAAGCTTCTGAAGAGGACCATACAAATGCCGCCTGCTTCGCCTGCATC
  CTCTTAAGCCATGGAGAAGAAAATATGGAATCTTGCTCTGTCACCCAGGCTGGAGTGCAGCGGCGTGATC
  TCGGAAGACTGCAACCTCCACCTCCCAGGCTTGCCGAGGGACCGAGCTTGATGATGGCATCCAGGCCGAC
  TCGGGGCCCATCAATGACACAGATGCTAATCCTCGATACAAGATCCCAGTGGAAGCTGACTTCCTCTTCG
  CCTATTCCACGGTTCCAGGCTATTACTCGTGGAGGAGCCCAGGAAGAGGCTCCTGGTTTGTGCAAGCCCT
  CTGCTCCATCCTGGAGGAGCACGGAAAAGACCTGGAAATCATGCAGATCCTCACCAGGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033340 unedited
  GGATTTTGTAATACGACTCACTATAGGGNNCGGCCGCGAAATTCGGCACGAGGGCAAGCT
  GGGCTGCTGGGTGGGTACTTCCTTCAAAGCTGAGGGAGCGTCCTACGCCCACGCGCGCAG
  GAGGGCGCCCCCCGCAAAGCAACGTCTAGGAGACCACAGTGGATGCCACAGCGGGCCCGA
  AGCGGATCAGCCTTGTGGGATGGCAGATGAGCAGGGCTGTATTGAAGAGCAGGGGGTTGA
  GGATTCAGCAAATGAAGATTCAGTGGATGCTAAGCCAGACCGGTCCTCGTTTGTACCGTC
  CCTCTTCAGTAAGAAGAAGAAAAATGTCACCATGCGATCCATCAAGACCACCCGGGACCG
  AGTGCCTACATATCAGTACAACATGAATTTTGAAAAGCTGGGCAAATGCATCATAATAAA
  CAACAAGAACTTTGATAAAGTGACAGGTATGGGCGTTCGAAACGGAACAGACAAAGATGC
  CGAGGCGCTCTTCAAGTGCTTCCGAAGCCTGGGTTTTGACGTGATTGTCTATAATGACTG
  CTCTTGTGCCAAGATGCAAGATCTGCTTAAAAAAGCTTCTGAAGAGGACCATACAAATGC
  CGCCTGCTTCGCCTGCATCCTCTTAAGCCATGGAGAAGAAAATGTAATTTATGGGAAAGA
  TGGTGTCACACCAATAAAGGATTTGACAGCCCACTTTANGGGGGATAGATGCAAAACCCT
  TTTAGAGAAACCCAAACTCTTCTTCATTCANGCTTGCCGNAGGACCGAGCTTGATGATGG
  CATCCAGGCCGACTCGGGGCCATCATGACACGATGCTAATCCTCGATACAAGATCCAGTG
  GAAGCTGACTTNCTCNTCGNCTATTNCACGGNNTCCAGCTATTACTCGTGGNAGAGCCCN
  AGAAAGAGCTNCTGGTTNGTGCAAGCCTCTGCTTCTNCTGNAGAGCCNGNAAAGACTGNA
  ATATGCAGACCTNACCAGGTGATGAACAGAGTGCAGCACTTTGAGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033340 unedited
  GTCCATCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTAAACAACCCGTGTTTAATCAATAAATATGAACCTTTGTT
  TTTTAACCCGTTACTATTAGGCAGAAAAAAACAGTCCTTTTTCAAGAATTCAAAAGATAA
  CCCCCTCTTGAAAACAAAGTGCCAAAAATCTTTGCCATAAACTCTTCCTCACTTATAGAA
  CTAAAAGTAACAGATTTTTACTCAAAATAAGGGTTGGAATAATCTAAAAAGTTCCAGATT
  TTTTTTAAAGCTATAACAGATTTTGCACATAAAGCCAAAACAGATTGGTTTACACATACC
  TAGACTATCTGACAACCCAATGAATAAATGATTGAATGGGTAAATTGGTGAGCCTTAGGG
  ATTACTTATGTTCACATTATGGGCCAGGCTTACATCCATTTCTTGGTCTTCTCCTGCCTC
  ACTGGCCCTTGCTCTTCCAAGGGCCTTGGGGAGGGGGCTTCCAAATAGGCCTGGGCAGTA
  AGGGGCTGGTGGAAAATGTGAGGGCAGGATAGGTGAGACCAAGGGATTCTCTTATCTGGC
  TCAACTCACTCCATCTCAGTCAGTGGTTTTGTTCTTGTCATGGCTCCATTTTCCACAATC
  CATTGGGTGGTCCTAACTCCCACTAGGCAGCCCCCACCATGGTTCCAGCTCCTCTGGATG
  ATCCCACTACTGGACACAGGAAACCAAACCTCTTCCCATTATCCTCATAGCCCTGGGGTG
  TGGTAACAGCTTCTTGCTGGTTGGCCTCCCTTTATCATCTATGAAGTCAATTTCCTGTAT
  TAAACTCCCTCTGGTGAAACCCCTAAATGGTTTCCTGTTTTCANACTGGATCCCTGATGA
  TACATTTCAGAATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033340
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033340.2, NP_203126.1
• RefSeq Size: 2433 bp
• RefSeq ORF: 762 bp
• Locus ID: 840
• UniProt ID: P55210
• Cytogenetics: 10q25.3
• Domains: Peptidase_C14
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis
• Gene Summary: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (b, also known as beta) has multiple differences in the 5' UTR and coding region, and initiates translation at a downstream in-frame start codon, compared to variant d. The encoded isoform (beta) is shorter and has a distinct C-terminus, compared to isoform delta.