CAMK2B (NM_172078) Human Untagged Clone

CAT#: SC109004

CAMK2B (untagged)-Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMK2B
• Synonyms: CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_172078 edited
  ATGGCCACCACGGTGACCTGCACCCGCTTCACCGACGAGTACCAGCTCTACGAGGATATT
  GGCAAGGGGGCTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGCACCGGCCATGAGTAT
  GCAGCCAAGATCATCAACACCAAGAAGCTGTCAGCCAGAGATCACCAGAAGCTGGAGAGA
  GAGGCTCGGATCTGCCGCCTTCTGAAGCATTCCAACATCGTGCGTCTCCACGACAGCATC
  TCCGAGGAGGGCTTCCACTACCTGGTCTTCGATCTGGTCACTGGTGGGGAGCTCTTTGAA
  GACATTGTGGCGAGAGAGTACTACAGCGAGGCTGATGCCAGTCACTGTATCCAGCAGATC
  CTGGAGGCCGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGACCTCAAGCCGGAG
  AACCTGCTTCTGGCCAGCAAGTGCAAAGGGGCTGCAGTGAAGCTGGCAGACTTCGGCCTA
  GCTATCGAGGTGCAGGGGGACCAGCAGGCATGGTTTGGTTTCGCTGGCACACCAGGCTAC
  CTGTCCCCTGAGGTCCTTCGCAAAGAGGCGTACGGCAAGCCCGTGGACATCTGGGCATGT
  GGGGTGATCCTGTACATCCTGCTCGTGGGCTACCCACCCTTCTGGGACGAGGACCAGCAC
  AAGCTGTACCAGCAGATCAAGGCTGGTGCCTATGACTTCCCGTCCCCTGAGTGGGACACC
  GTCACTCCTGAAGCCAAAAACCTCATCAACCAGATGCTGACCATCAACCCTGCCAAGCGC
  ATCACAGCCCATGAGGCCCTGAAGCACCCGTGGGTCTGCCAACGCTCCACGGTAGCATCC
  ATGATGCACAGACAGGAGACTGTGGAGTGTCTGAAAAAGTTCAATGCCAGGAGAAAGCTC
  AAGGGAGCCATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTC
  AACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCC
  ACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCAT
  AACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAA
  GACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTC
  AACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAG
  CCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAAC
  CTGCTGGCCAAGAACAGCAAGCCGATCCACACGACCATCCTGAACCCACACGTGCACGTC
  ATTGGAGAGGATGCCGCCTGCATCGCTTACATCCGGCTCACGCAGTACATTGACGGGCAG
  GGCCGGCCCCGCACCAGCCAGTCTGAGGAGACCCGCGTGTGGCACCGCCGCGACGGCAAG
  TGGCAGAACGTGCACTTCCACTGCTCGGGCGCGCCTGTGGCCCCGCTGCAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172078 unedited
  CAAAACCCTTCGCACGAGGCGGGAGCCGGAGTCGCCGCCGCCCGAGCGCAGCCGAGCGCA
  CGCCGAGCCCGTCCGCCGCCGCCATGGCCACCACGGTGACCTGCACCCGCTTCACCGACG
  AGTACCAGCTCTACGAGGATATTGGCAAGGGGGCTTTCTCTGTGGTCCGACGCTGTGTCA
  GTTCTGCACCGGCCATGAGTATGCAGCCAAGATCATCAACACCAAGAAGCTGTCAGCCAG
  AGATCACCAGAAGCTGGAGAGAGAGGCTCGGATCTGCCGCCTTCTGAAGCATTCCAACAT
  CGTGCGTCTCCACGACAGCATCTCCGAGGAGGGCTTCCACTACCTGGTCTTCGATCTGGT
  CACTGGTGGGGAGCTCTTTGAAGACATTGTGGCGAGAGAGTACTACAGCGAGGCTGATGC
  CAGTCACTGTATCCAGCAGATCCTGGAGGCCGTTCTCCATTGTCACCAAATGGGGGTCGT
  CCACAGAGACCTCAAGCCGGAGAACCTGCTTCTGGCCAGCAAGTGCAAAGGGGCTGCAGT
  GAAGCTGGCAGACTTCGGCCTAGCTATCGAGGTGCAGGGGGACCAGCAGGCATGGTTTGG
  TTTCGCTGGCACACCAGGCTACCTGTCCCCCTGAGTCCTTCGCAAAGAGGCGTACGGCAA
  GCCCGTGGACATCTGGGCATGTGGGGTGATCCTGTACATCCTGCTCGTGGGCTACCCACC
  CTTCTGGGACGAAGACCAGCACAAGCTGTACCAGCAGATCAAGGCTTGTGCCTATGACTT
  CCCGTCCCCTGATTGGAACACGTCACTTCCTGAGCCAAAACCTCATTAACAAATGCTTGC
  CATAAACCTGCCAAGCGCATAAAAGCCCATGAGGCCCTGGAACCCCCGTGGTTTGCCAAC
  CCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172078 unedited
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTTTTTAACAAATCACATCTGG
  TCTTGTTTTTCTGCAGACACAGGCACCAGGGGAGGGAACGAGGCAGACACAAACATGCGA
  CAGGCAGGCCGTGTGCCCGAGGGCAGTCGGGCTCCAAACACCAACTCTGTCCGGCGAAAC
  CAGGGCGCAGCTCTTCACTGCAGCGGGGCCACAGGCGCGCCCGAGCAGTGGAAGTGCACG
  TTCTGCCACTTGCCGTCGCGGCGGTGCCACACGCGGGTCTCCTCAGACTGGCTGGTGCGG
  GGCCGGCCCTGCCCGTCAATGTACTGCGTGAGCCGGATGTAAGCGATGCAGGCGGCATCC
  TCTCCAATGACGTGCACGTGTGGGTTCAGGATGGTCGTGTGGATCGGCTTGCTGTTCTTG
  GCCAGCAGGTTCTCGAAGTAGAATCTGTGGAAGTCCATCCCTTCAACCAGGTTGCCCAGT
  GCTTCAGGCTCAAACGAGGTCAGCCCTGGGTCACAGATTTTCGCGTAGGCCTCAAAGTCA
  CCGTTGTTGACGGCCTCGATGAGCTGCTCCGTGGTCTTAATGATCTCCTGCTTCCGGGCT
  TTAGCGTCTTCATCCTCTATGGTGGTATTGGCACTGTCAGAAGACTCCTTAATCCCGTCC
  ACTGNGNTATGGATGACGGTGGTTTGAGGCTCCAGGGCGGCAGGAGGAAGCGTCCCTTTG
  GGCTCGTGGCGCTGCACTGTTTTTGGGCTATTCGTCTGGGGCTTGACTCCATCTGCTTTC
  TTGTTGAGTAAACTCTTGGCTGAAAAATTCCGTGTGGCCAGCATTGTTGTGAAGGATGGC
  CTCCTTTGACCTTCCTCCTGCATTGACCTTTTCAGAACCTCCCAATCTCCTGGTTGGCAT
  CAAGCAGCTACGGGGAACGTTGGCAAACCACCGTGCCCACGGCCTAAGGGCCGTAAGCCT
  TGCCAGGGTGATGCACCATGTGGGAGCAAGCTTTGGCTAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_172078
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172078.1, NP_742075.1
• RefSeq Size: 2066 bp
• RefSeq ORF: 1629 bp
• Locus ID: 816
• UniProt ID: Q13554
• Cytogenetics: 7p13
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
• Gene Summary: The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (2) that is missing an internal segment compared to isoform 1. Variants 2 and 9 encode the same protein (isoform 2). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.