CAMK2A (NM_171825) Human Untagged Clone

CAT#: SC109000

CAMK2A (untagged)-Human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMK2A
• Synonyms: CAMKA; CaMKIIalpha; CaMKIINalpha; MRD53; MRT63
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109000 sequence for NM_171825 edited (data generated by NextGen Sequencing)
  ATGGCCACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGC
  AAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCT
  GCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAA
  GCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCA
  GAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGAT
  ATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTG
  GAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAAT
  CTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGGCC
  ATAGAGGTGGAGGGGGAGCAGCAGGCATGGTTTGGGTTTGCAGGGACTCCTGGATATCTC
  TCCCCAGAAGTGCTGCGGAAGGACCCGTACGGGAAGCCTGTGGACCTGTGGGCTTGTGGG
  GTCATCCTGTACATCCTGCTGGTTGGGTACCCCCCGTTCTGGGATGAGGACCAGCACCGC
  CTGTACCAGCAGATCAAAGCCGGCGCCTATGATTTCCCATCGCCGGAATGGGACACTGTC
  ACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATTAACCCATCCAAACGCATC
  ACAGCTGCCGAAGCCCTTAAGCACCCCTGGATCTCGCACCGCTCCACCGTGGCATCCTGC
  ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAG
  GGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGA
  AACAAGAAGAGCGATGGTGTGAAGGAATCCTCAGAGAGCACCAACACCACCATCGAGGAT
  GAAGACACCAAAGTGCGGAAACAGGAAATTATAAAAGTGACAGAGCAGCTGATTGAAGCC
  ATAAGCAATGGAGATTTTGAGTCCTACACGAAGATGTGCGACCCTGGCATGACAGCCTTC
  GAACCTGAGGCCCTGGGGAACCTGGTTGAGGGCCTGGACTTCCATCGATTCTATTTTGAA
  AACCTGTGGTCCCGGAACAGCAAGCCCGTGCACACCACCATCCTGAATCCCCACATCCAC
  CTGATGGGCGACGAGTCAGCCTGCATCGCCTACATCCGCATCACGCAGTACCTGGACGCT
  GGCGGCATCCCACGCACCGCCCAGTCGGAGGAGACCCGTGTCTGGCACCGCCGGGATGGC
  AAATGGCAGATCGTCCACTTCCACAGATCTGGGGCGCCCTCCGTCCTGCCCCACTGA
  
  Clone variation with respect to NM_171825.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_171825 unedited
  GGGGTGGGGGTTAAATAATANAATATAAANNNTTTCTTTCCCCCGCCCGTTGCCGCATTG
  GGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGACACTATA
  GAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGTCAGTCAAA
  CCGGTTCTCTGTTTGCACTCGGCAGCACGGGCAGGCAAGTGGTCCCTAGGTTCGGGAGCA
  GAGCAGCAGCGCCTCAGTCCTGGTCCCCCAGTCCCAAGCCTCACCTGCCTGCCCAGCGCC
  AGGATGGCCACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTG
  GGCAAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTAT
  GCTGCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGT
  GAAGCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATC
  TCAGAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAA
  GATATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATC
  CTGGAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAG
  AATCTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTG
  GCCATAGAGGTGGAGGGGGAGCACCAGGCATGTTTTGGGTTTGCAGGGACTCCTGGATAT
  CTCTCCCCAAAGTGCTGCGGAAGGACCCGTACGGAAGCCTGTGGACCTGTGGCCTTGTGG
  GGTCATCCTGTCATCCTGCCTGTTGGGTCCCCCCCGTCTGGGATAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_171825 unedited
  AACCCTAGGGATGGAAACTCCCCATTCCAGTATAGCACTGGGGNAGGGTCACAGGGCTGC
  CACCCGGGTTCTGTTCAGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCACAAAAGTTTGTAGCTTTTTATTGCACT
  GAAAACACCAAGAATAAAACAAACGCCCCTTTTTTTTATTCCACCCCCCCCCCAATAGCA
  AAAAGTTTGAGCAGTGTTCATTCAAGTTCACAGCCACCTTTTTTTAAAAAAGAAAAGAAA
  AAAAAAAAAGAACTTAAACAAAACCCCAAACAAATTGTAACAAAGAAACCAGCACGGGGA
  GTCTGGCAAACTCATCCCCCAAAAGGGTCTCCCTTTGAAGGGAGACAGGAGGCCTTGGGT
  CAGGATTCGCACCATGGTGGGCACAAACCCAGGAAAACACTTTCCTGTAAACGTGTTTTC
  ATGCTGGAGCCAAGGTTTTGACTTGGGGTNGGATTTTTATTTATTTATTTATTATTATTA
  TTATTATTTCGCAACCAAAGGATGTTTTGGAAGAACACAAATTTTGTCTGGGGAGGGTAG
  GCTCTGGGCAAATTTTTCTGTGAATCTCCCCTGCCTGGCGCATTAAGATCATCCCTGGTG
  CCCTGTGGTGGCACCAGGTGGCCTGCCCACCCACAGCGGTGCCCTTCACATGGGGGCCAT
  TTTAGCCGTGGGGGAAAGATTTGGCTCCACTTTTCTGGCACTTTGGAGATGGACCATTGG
  ACAAGGAGTGTTGTTAACCCAATGAAGGGAAGGACCTCGAGCTCCTTCCACTTTATTTCC
  TTTGGGGGGGGAAAACCAAACCCCAAAAAGGGGAAGGGAATTGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_171825
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_171825.1, NP_741960.1
• RefSeq Size: 4803 bp
• RefSeq ORF: 1437 bp
• Locus ID: 815
• UniProt ID: Q9UQM7
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
• Gene Summary: The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 3. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Variants 2 and 4 both encode the same isoform (2). Isoforms 2 and 5 are the same length but differ in sequence. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.