TGF beta Receptor I (TGFBR1) (NM_004612) Human Untagged Clone

CAT#: SC108520

TGFBR1 (untagged)-Human transforming growth factor, beta receptor 1 (TGFBR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGF beta Receptor I
• Synonyms: AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1; LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004612 edited
  ATGGAGGCGGCGGTCGCTGCTCCGCGTCCCCGGCTGCTCCTCCTCGTGCTGGCGGCGGCG
  GCGGCGGCGGCGGCGGCGCTGCTCCCGGGGGCGACGGCGTTACAGTGTTTCTGCCACCTC
  TGTACAAAAGACAATTTTACTTGTGTGACAGATGGGCTCTGCTTTGTCTCTGTCACAGAG
  ACCACAGACAAAGTTATACACAACAGCATGTGTATAGCTGAAATTGACTTAATTCCTCGA
  GATAGGCCGTTTGTATGTGCACCCTCTTCAAAAACTGGGTCTGTGACTACAACATATTGC
  TGCAATCAGGACCATTGCAATAAAATAGAACTTCCAACTACTGTAAAGTCATCACCTGGC
  CTTGGTCCTGTGGAACTGGCAGCTGTCATTGCTGGACCAGTGTGCTTCGTCTGCATCTCA
  CTCATGTTGATGGTCTATATCTGCCACAACCGCACTGTCATTCACCATCGAGTGCCAAAT
  GAAGAGGACCCTTCATTAGATCGCCCTTTTATTTCAGAGGGTACTACGTTGAAAGACTTA
  ATTTATGATATGACAACGTCAGGTTCTGGCTCAGGTTTACCATTGCTTGTTCAGAGAACA
  ATTGCGAGAACTATTGTGTTACAAGAAAGCATTGGCAAAGGTCGATTTGGAGAAGTTTGG
  AGAGGAAAGTGGCGGGGAGAAGAAGTTGCTGTTAAGATATTCTCCTCTAGAGAAGAACGT
  TCGTGGTTCCGTGAGGCAGAGATTTATCAAACTGTAATGTTACGTCATGAAAACATCCTG
  GGATTTATAGCAGCAGACAATAAAGACAATGGTACTTGGACTCAGCTCTGGTTGGTGTCA
  GATTATCATGAGCATGGATCCCTTTTTGATTACTTAAACAGATACACAGTTACTGTGGAA
  GGAATGATAAAACTTGCTCTGTCCACGGCGAGCGGTCTTGCCCATCTTCACATGGAGATT
  GTTGGTACCCAAGGAAAGCCAGCCATTGCTCATAGAGATTTGAAATCAAAGAATATCTTG
  GTAAAGAAGAATGGAACTTGCTGTATTGCAGACTTAGGACTGGCAGTAAGACATGATTCA
  GCCACAGATACCATTGATATTGCTCCAAACCACAGAGTGGGAACAAAAAGGTACATGGCC
  CCTGAAGTTCTCGATGATTCCATAAATATGAAACATTTTGAATCCTTCAAACGTGCTGAC
  ATCTATGCAATGGGCTTAGTATTCTGGGAAATTGCTCGACGATGTTCCATTGGTGGAATT
  CATGAAGATTACCAACTGCCTTATTATGATCTTGTACCTTCTGACCCATCAGTTGAAGAA
  ATGAGAAAAGTTGTTTGTGAACAGAAGTTAAGGCCAAATATCCCAAACAGATGGCAGAGC
  TGTGAAGCCTTGAGAGTAATGGCTAAAATTATGAGAGAATGTTGGTATGCCAATGGAGCA
  GCTAGGCTTACAGCATTGCGGATTAAGAAAACATTATCGCAACTCAGTCAACAGGAAGGC
  ATCAAAATGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004612 unedited
  CAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGNAATTCGCACCAGGCGAGGTTT
  GCTGGGGTGAGGCAGCGGCGCGGCCGGGCCGGGCCGGGCCACAGGCGGTGGCGGCGGGAC
  CATGGAGGCGGCGGTCGCTGCTCCGCGTCCCCGGCTGCTCCTCCTCGTGCTGGCGGCGGC
  GGCGGCGGCGGCGGCGGCGCTGCTCCCGGGGGCGACGGCGTTACAGTGTTTCTGCCACCT
  CTGTACAAAAGACAATTTTACTTGTGTGACAGATGGGCTCTGCTTTGTCTCTGTCACAGA
  GACCACAGACAAAGTTATACACAACAGCATGTGTATAGCTGAAATTGACTTAATTCCTCG
  AGATAGGCCGTTTGTATGTGCACCCTCTTCAAAAACTGGGTCTGTGACTACAACATATTG
  CTGCAATCAGGACCATTGCAATAAAATAGAACTTCCAACTACTGTAAAGTCATCACCTGG
  CCTTGGTCCTGTGGAACTGGCAGCTGTCATTGCTGGACCAGTGTGCTTCGTCTGCATCTC
  ACTCATGTTGATGGTCTATATCTGCCACAACCGCACTGTCATTCACCATCGAGTGCCAAA
  TGAAGAGGACCCTTCATTAGATCGCCCTTTTATTTCAGAGGGTACTACGTTGAAAGACTT
  AATTTATGATATGACAACGTCAGGTTCTGGCACAGGTTTACCATTGCTTGTTCAGAGAAC
  AATTGCGAGAACTATTGTGTTACAAGAAAGCATTGGCAAAGGTCGATTTGGAGAAGTTTG
  GAGAGGAAAGTGGCGGGNGAGAAGAAGTTGCTGTTAAGATATTCTCCTCTAGAGAAGACG
  TTCGTGGTTCCGTGAGGCAGAGATTNNATCAAACTGTATGTTACGTCATGAAAACATCCT
  GNGATTATAGCAGCAGACATAAAGACATGGTACTGGNACTCGCTCTGGNTGTGGCAGATA
  TCAGAGCAGGATACCTTTTGGATACTAACAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004612 unedited
  GCCCAATAGGGGTATTGAATTCCATATTACTGCTGCAAAAGAAAGCCTATCCTTCTGTTC
  CCTCTCATGAGGTAGAACAATTGACCTCCCAAATTAAAACCCAGGAGCAGATCTGAAGAA
  AAAAGGAGAGTTCAGGCAAAGCTGTAGAATTACATTTTGATGCCTTCCTGTTGACTGAGT
  TGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTATCTGCTCCATTGGCATACCAA
  CATTCTCTCATAATTTTAGCCATTACTCTCAAGGCTTCACAGCTCTGCCATCTGTTTGGG
  ATATTTGGCCTTAACTTCTGTTCACAAACAACTTTTCTCATTTCTTCAACTGATGGGTCA
  GAAGGTACAAGATCATAATAAGGCAGTTGGTAATCTTCATGAATTCCACCAATGGAACAT
  CGTCGAGCAATTTCCCAGAATACTAAGCCCATTGCATAGATGTCAGCACGTTTGAAGGAT
  TCAAAATGTTTCATATTTATGGAATCATCGAGAACTTCAGGGGCCATGTACCTTTTTGTT
  CCCACTCTGTGGTTTGGAGCAATATCAATGGTATCTGTGGCTGAATCATGTCTTACTGCC
  AGTCCTAAGTCTGCAATACAGCAAGTTCCATTCTTCTTTACCAAGATATTCTTTGATTTC
  AAATCTCTATGAGCAATGGCTGGCTTTCCTTGGGTACCAACAATCTCCATGTGAAGATGG
  GCAAGACCGCTCGCCGTGGACAGAGCAAGTTTTATCATTCCTTCCACAGTAACTGTGTAT
  CTGTTTAAGTAATCAAAAGGGATCCATGCTCATGATAATCTGACACCACCAGAGCTGAGT
  CCAATACCATTGTCTTTATTGTCTGCTGCTATAAATCCCAAGATGTTTTCATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004612
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004612.2, NP_004603.1
• RefSeq Size: 6475 bp
• RefSeq ORF: 1512 bp
• Locus ID: 7046
• UniProt ID: P36897
• Cytogenetics: 9q22.33
• Domains: Activin_recp, pkinase, TyrKc, S_TKc, GS
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 3. The encoded isoform (1) is shorter than isoform 3.