DBF4 (NM_006716) Human Untagged Clone

CAT#: SC108298

DBF4 (untagged)-Human DBF4 homolog (S. cerevisiae) (DBF4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBF4
• Synonyms: ASK; CHIF; DBF4A; ZDBF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108298 sequence for NM_006716 edited (data generated by NextGen Sequencing)
  ATGAACTCCGGAGCCATGAGGATCCACAGTAAAGGACATTTCCAGGGTGGAATCCAAGTC
  AAAAATGAAAAAAACAGACCATCTCTGAAATCTCTGAAAACTGATAACAGGCCAGAAAAA
  TCCAAATGTAAGCCACTTTGGGGAAAAGTATTTTACCTTGACTTACCTTCTGTCACCATA
  TCTGAAAAACTTCAAAAGGACATTAAGGATCTGGGAGGGCGAGTTGAAGAATTTCTCAGC
  AAAGATATCAGTTATCTTATTTCAAATAAGAAGGAAGCTAAATTTGCACAAACCTTGGGT
  CGAATTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCACTTCACCTCAT
  CCCAGCCATGATGGAAGTTCATTTAAGTCACCAGACACAGTSTGTTTAAGCAGAGGAAAA
  TTATTAGTTGAAAAAGCTATCAAGGACCATGATTTTATTCCTTCAAATAGTATATTATCA
  AATGCCTTGTCATGGGGAGTAAAAATTCTTCATATTGATGACATTAGATACTACATTGAA
  CAAAAGAAAAAAGAGTTGTATTTACTCAAGAAATCAAGTACTTCAGTAAGAGATGGGGGC
  AAAAGAGTTGGTAGTGGTGCACAAAAAACAAGAACAGGAAGACTCAAAAAGCCTTTTGTA
  AAGGTGGAAGATATGAGCCAACTTTATAGGCCATTTTATCTTCAGCTGACCAATATGCCT
  TTTATAAATTATTCTATTCAGAAGCCCTGCAGTCCATTTGATGTAGACAAGCCATCTAGT
  ATGCAAAAGCAAACTCAGGTTAAACTAAGAATCCAAACAGATGGCGATAAGTATGGTGGA
  ACCTCAATTCAACTCCAGTTGAAAGAGAAGAAGAAAAAAGGATATTGTGAATGTTGCTTG
  CAGAAATATGAAGATCTAGAAACTCACCTTCTAAGTGAGCAACACAGAAACTTTGCACAG
  AGTAACCAGTATCAAGTTGTTGATGATATTGTATCTAAGTTAGTTTTTGACTTTGTGGAA
  TATGAAAAGGACACACCTAAAAAGAAAAGAATAAAATACAGTGTTGGATCCCTTTCTCCT
  GTTTCTGCAAGTGTCCTGAAAAAGACTGAACAAAAGGAAAAAGTGGAATTGCAACATATT
  TCTCAGAAAGATTGCCAGGAAGATGATACAACAGTGAAGGAGCAGAATTTCCTGTATAAA
  GAGACCCAGGAAACTGAAAAAAAGCTCCTGTTTATTTCAGAGCCCATCCCCCACCCTTCA
  AATGAATTGAGAGGGCTTAATGAGAAAATGAGTAATAAATGTTCCATGTTAAGTACAGCT
  GAAGATGACATAAGACAGAATTTTACACAGCTACCTCTACATAAAAACAAACAGGAATGC
  ATTCTTGACATTTCCGAACACACATTAAGTGAAAATGACTTAGAAGAACTAAGGGTAGAT
  CACTATAAATGTAACATACAGGCATCTGTACATGTTTCTGATTTCAGTACAGATAATAGT
  GGATCTCAACCAAAACAGAAGTCAGATACTGTGCTTTTTCCAGCAAAGGATCTCAAGGAA
  AAGGACCTTCATTCAATATTTACTCATGATTCTGGTCTGATAACAATAAACAGTTCACAA
  GAGCACCTAACTGTTCAGGCAAAGGCTCCATTCCATACTCCTCCTGAGGAACCCAATGAA
  TGTGACTTCAAGAATATGGATAGTTTACCTTCTGGTAAAATACATCGAAAAGTGAAAATA
  ATATTAGGACGAAATAGAAAAGAAAATCTGGAACCAAATGCTGAATTTGATAAAAGAACT
  GAATTTATTACACAAGAAGAAAACAGAATTTGTAGTTCACCGGTACAGTCTTTACTAGAC
  TTGTTTCAGACTAGTGAAGAGAAATCAGAATTTTTGGGTTTCACAAGCTACACAGAAAAG
  AGTGGTATATGCAATGTTTTAGATATTTGGGAAGAGGAAAATTCAGATAATCTGTTAACA
  GCGTTTTTCTCGTCCCCTTCAACTTCTACATTTACTGGCTTTTAG
  
  Clone variation with respect to NM_006716.3
  402 g=>s
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006716 unedited
  TTGGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGAAAGAGAG
  AGGCGGCCGTCCTGTCAACAGGCCGGGGGAAGCCGTGCTTTCGCGGCTGCCCGGTGCGAC
  ACTTTCTCCGGACCCAGCATGTAGGTGCCGGGCGACTGCCATGAACTCCGGAGCCATGAG
  GATCCACAGTAAAGGACATTTCCAGGGTGGAATCCAAGTCAAAAATGAAAAAAACAGACC
  ATCTCTGAAATCTCTGAAAACTGATAACAGGCCAGAAAAATCCAAATGTAAGCCACTTTG
  GGGAAAAGTATTTTACCTTGACTTACCTTCTGTCACCATATCTGAAAAACTTCAAAAGGA
  CATTAAGGATCTGGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATCTTAT
  TTCAAATAAGAAGGAAGCTAAATTTGCACAAACCTTGGGTCGAATTTCTCCTGTACCAAG
  TCCAGAATCTGCATATACTGCAGAAACCACTTCACCTCATCCCAGCCATGATGGAAGTTC
  ATTTAAGTCACCAGACACAGTCTTTTTATAGGTGTGTTTAAGCAGAGGAAAATTATTAGT
  TGAAAAAGCTATCAAGGACCATGATTTTATTCCTTCAAATAGTATATTATCAAATGCCTT
  GTCATGGGGAGTAAAAATTCTTCATATTGATGACATTAGATACTACATTGAACAAAAGAA
  AAAAGAGTTGTATTTACTCAAGAAATCAAGTACTTCAGTAAGAGATGGGGGCAAAAGAGT
  TGGTAGTGGTGCACAAAANNACAGAACAGGAAGACTCANAAAGCCTTTTGTAANGGTGGA
  AGATATGAGCCCACTTATAGGGCCATTTATCTTCAGCTGACCATATGCCTTTATNAANTA
  TNCTATCAGAAGCCTGCAGTCATNTGATGTGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006716 unedited
  GGCCCTACTGTGNACCGCGGNCCGCTATTTNANGATCGNGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTAATGTAAAACAAAACCAGTCACAAGTTTATTCTGTAATTTTTCTTTAACAG
  GTATTCAATTCTGTAAAAAATTGCAAATATTTATTTTTAATATTTACATTTGGGTCTGTA
  AACAAAGCTGGTAAAAAAATCCTAAAAATTTCCATACATATTTATAAAAATTTCAAGAAT
  ATGATCCTTATCACTTCTGAAAAGTATGCATTTTTTAAATTCTAAAAGCCAGTAAATGTA
  AAAGTTGAAGGGGACGAAAAAAACGCTGTTAACAGATTATCTGAATTTTCCTCTTCCCAA
  ATATCTAAAACATTGCATATACCACTCTTTTCTGTGTAGCTTGTGAAACCCAAAAATTCT
  GATTTCTCTTCACTAGTCTGAAACAAGTCTAGTAAAGACTGTACCGGTGAACTACAAATT
  CTGTTTTCTTCTTGTGTAATAAATTCAGTTCTTTTATCAAATTCAGCATTTGGTTCCAGA
  TTTTCTTTTCTATTTCGTCCTAATATTATTTTCACTTTTCGATGTATTTTACCAGAAGGT
  AAACTATCCATATTCTTGAAGTCACATTCATTGGGTTCCTCAGGAGGAGTATGGAATGGA
  GCCTTTGCCTGAACAGTTAGGTGCTCTTGTGAACTGTTTATTGTTATCAGACCAGAATCA
  TGAGTAAATATTGAATGAAGGTCCTTTTCCTTGAGATCCTTTGCTGGAAAAACACAGTAT
  CTGACTTCTGTTTTGGTTGAGATCCACTATTATCTGTACTGAAATCAGNACATGTACAGA
  TGCCTGTATGTTACATTTATAGNGATCTACCCTTAGNTNCTTCTAGNNCATTNTCACTTA
  ATGNGNNGTCCGAAAATGTCAAGATGCATTCCTGNTTGTGTTTATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006716
• Insert Size: 2710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006716.3, NP_006707.1
• RefSeq Size: 3923 bp
• RefSeq ORF: 2025 bp
• Locus ID: 10926
• UniProt ID: Q9UBU7
• Cytogenetics: 7q21.12
• Domains: ZnF_DBF
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle
• Gene Summary: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).