B7-1 (CD80) (NM_005191) Human Untagged Clone

CAT#: SC108276

CD80 (untagged)-Human CD80 molecule (CD80)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B7-1
• Synonyms: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108276 sequence for NM_005191 edited (data generated by NextGen Sequencing)
  ATGGGCCACACACGGAGGCAGGGAACATCACCATCCAAGTGTCCATACCTCAATTTCTTT
  CAGCTCTTGGTGCTGGCTGGTCTTTCTCACTTCTGTTCAGGTGTTATCCACGTGACCAAG
  GAAGTGAAAGAAGTGGCAACGCTGTCCTGTGGTCACAATGTTTCTGTTGAAGAGCTGGCA
  CAAACTCGCATCTACTGGCAAAAGGAGAAGAAAATGGTGCTGACTATGATGTCTGGGGAC
  ATGAATATATGGCCCGAGTACAAGAACCGGACCATCTTTGATATCACTAATAACCTCTCC
  ATTGTGATCCTGGCTCTGCGCCCATCTGACGAGGGCACATACGAGTGTGTTGTTCTGAAG
  TATGAAAAAGACGCTTTCAAGCGGGAACACCTGGCTGAAGTGACGTTATCAGTCAAAGCT
  GACTTCCCTACACCTAGTATATCTGACTTTGAAATTCCAACTTCTAATATTAGAAGGATA
  ATTTGCTCAACCTCTGGAGGTTTTCCAGAGCCTCACCTCTCCTGGTTGGAAAATGGAGAA
  GAATTAAATGCCATCAACACAACAGTTTCCCAAGATCCTGAAACTGAGCTCTATGCTGTT
  AGCAGCAAACTGGATTTCAATATGACAACCAACCACAGCTTCATGTGTCTCATCAAGTAT
  GGACATTTAAGAGTGAATCAGACCTTCAACTGGAATACAACCAAGCAAGAGCATTTTCCT
  GATAACCTGCTCCCATCCTGGGCCATTACCTTAATCTCAGTAAATGGAATTTTTGTGATA
  TGCTGCCTGACCTACTGCTTTGCCCCAAGATGCAGAGAGAGAAGGAGGAATGAGAGATTG
  AGAAGGGAAAGTGTACGCCCTGTATAA
  
  Clone variation with respect to NM_005191.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005191 unedited
  ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAACATCTCAGAAGTGGAGTCTT
  ACCCTGAAATCAAAGGATTTAAAGAAAAAGTGGAATTTTTCTTCAGCAAGCTGTGAAACT
  AAATCCACAACCTTTGGAGACCCAGGAACACCCTCCAATCTCTGTGTGTTTTGTAAACAT
  CACTGGAGGGTCTTCTACGTGAGCAATTGGATTGTCATCAGCCCTGCCTGTTTTGCACCT
  GGGAAGTGCCCTGGTCTTACTTGGGTCCAAATTGTTGGCTTTCACTTTTGACCCTAAGCA
  TCTGAAGCCATGGGCCACACACGGAGGCAGGGAACATCACCATCCAAGTGTCCATACCTC
  AATTTCTTTCAGCTCTTGGTGCTGGCTGGTCTTTCTCACTTCTGTTCAGGTGTTATCCAC
  GTGACCAAGGAAGTGAAAGAAGTGGCAACGCTGTCCTGTGGTCACAATGTTTCTGTTGAA
  GAGCTGGCACAAACTCGCATCTACTGGCAAAAGGAGAAGAAAATGGTGCTGACTATGATG
  TCTGCGGACATGAATATATGGCCCGAGTACAAGAACCGGACCATCTTTGATATCACTAAT
  AACCTCTCCATTGTGATCCTGGCTCTGCGCCCATCTGACGAGGGCACATACGAGTGCGTT
  GTTCCTGAGTATGAAAAAGACGCTTTCAAGCGGGAACACCTGCCTGAAGTGACGTTATCA
  GTCAAAGCTGACTTTCCTACACCTAGAATATCCGACTTTGAAATTCCCACTTCTAATATT
  AGAAGGAATATTTGCTCCACCTCTGGGAGCTTTCCCGAGCCTCACCTCTCCCTGGCTGGA
  AAAGGGAGAAAATTAAATGCCTCAACCCACAGCTTCCCAGATCCTGAAATGACCTTATGC
  TGTTACAGAAACTGGATTCATTTGAAACCAACCCAGCTCTGGGTTCATTAGCATGGCTCT
  TAAATGGATACACCTCAACGAGAACAACAAGCAGAAATTTCTGATAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005191 unedited
  TGGGTGGGGCTGCTGATCNCGACTTTACTTATCCCTCATTTAAATACTACTTATGTTTGT
  ATTAATCTATCAATATATGCATACATGAATATATCCACCCACCTAGATTTTAAGCAGTAA
  ATAAAACATTTCGCAAAAGATTAAAGTTGAATTTTACAGTTCGTATATTCATGTGGTCCT
  TTGAAAGGGTATTCTAGAAATCACTGTTTTAGAGGAGAGGAAAGAACCAGGTAGGCAAAT
  GGTCTGTGAAACCCTTGGGTCCTGGAAGCAGTGTGAGTGTAAATGTGTAGTGTTTGGTTT
  CATCTAAATAAACAAAGATGATTTCTTTGACACTTGAAATAAAATACAAATTCAACAAAA
  AGTAGATCAGCATTATTAAAGAAACGGTTCAACTTTGTTTCTTCCCTTAGTATTGCTGAC
  AAAGTATCTGCTGTAGAATACAGGAATTACTTAGAATAGAAACATAGTCATCACAACTGT
  TACTAAATGGAAAAGAAAAGAATTATTGAGTTAAGTATTCCTGTCAATACGGGAAACACT
  GCTAGTACCTTATGTTGGTGTTAGACCTCTCTGCCTACACTGAGAATATAGTTTTACACA
  GGAGCAAGTTTTGTGAAGCAGCATAGTGAGGTAGCTAAAGCCATGGGCTGGCTCTAAAGG
  CTTTAAAATCCCACCCTGTGGGCTAACCTGCCATGAGATGTGCATTTGAAAAATGTTGTC
  TTTCTTTTGCTGTTCAACTCCCAATTTCCAAATGAAAATGTGATTATTCCACCTAAAGTT
  GCGCCCCTTCCTGTCTAGTGAAATGGGGAAGGCCATTTTAAAGAAAGGGATCTGGGTAAC
  ATGGACACGCGTTGGCTTTGCCTTCCCGTTTCTGGCGGAGTCGAAAAACCCAAATTCCCA
  GGGAAAAAACCTTCTTAGGTTCTAAGGAGCCCTTGGAAAGTTCTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005191
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005191.2, NP_005182.1
• RefSeq Size: 2824 bp
• RefSeq ORF: 2757 bp
• Locus ID: 941
• UniProt ID: P33681
• Cytogenetics: 3q13.33
• Domains: ig
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]