Junctional Adhesion Molecule 2 (JAM2) (NM_021219) Human Untagged Clone

CAT#: SC108217

JAM2 (untagged)-Human junctional adhesion molecule 2 (JAM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Junctional Adhesion Molecule 2
• Synonyms: C21orf43; CD322; IBGC8; JAM-B; JAMB; PRO245; VE-JAM; VEJAM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108217 sequence for NM_021219 edited (data generated by NextGen Sequencing)
  ATGGCGAGGAGGAGCCGCCACCGCCTCCTCCTGCTGCTGCTGCGCTACCTGGTGGTCGCC
  CTGGGCTATCATAAGGCCTATGGGTTTTCTGCCCCAAAAGACCAACAAGTAGTCACAGCA
  GTAGAGTACCAAGAGGCTATTTTAGCCTGCAAAACCCCAAAGAAGACTGTTTCCTCCAGA
  TTAGAGTGGAAGAAACTGGGTCGGAGTGTCTCCTTTGTCTACTATCAACAGACTCTTCAA
  GGTGATTTTAAAAATCGAGCTGAGATGATAGATTTCAATATCCGGATCAAAAATGTGACA
  AGAAGTGATGCGGGGAAATATCGTTGTGAAGTTAGTGCCCCATCTGAGCAAGGCCAAAAC
  CTGGAAGAGGATACAGTCACTCTGGAAGTATTAGTGGCTCCAGCAGTTCCATCATGTGAA
  GTACCCTCTTCTGCTCTGAGTGGAACTGTGGTAGAGCTACGATGTCAAGACAAAGAAGGG
  AATCCAGCTCCTGAATACACATGGTTTAAGGATGGCATCCGTTTGCTAGAAAATCCCAGA
  CTTGGCTCCCAAAGCACCAACAGCTCATACACAATGAATACAAAAACTGGAACTCTGCAA
  TTTAATACTGTTTCCAAACTGGACACTGGAGAATATTCCTGTGAAGCCCGCAATTCTGTT
  GGATATCGCAGGTGTCCTGGGAAACGAATGCAAGTAGATGATCTCAACATAAGTGGCATC
  ATAGCAGCCGTAGTAGTTGTGGCCTTAGTGATTTCCGTTTGTGGCCTTGGTGTATGCTAT
  GCTCAGAGGAAAGGCTACTTTTCAAAAGAAACCTCCTTCCAGAAGAGTAATTCTTCATCT
  AAAGCCACGACAATGAGTGAAAATGATTTCAAGCACACAAAATCCTTTATAATTTAA
  
  Clone variation with respect to NM_021219.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021219 unedited
  GTTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACGAGGCGTCCCTCCT
  CTTCCTCCCCTCCCGACTCTCTGCTCCTTTCCCGCCCCANAAGTTCAAGGGCCCCCGGCC
  TCCTGCGCTCCTGCCGCCGGGACCCTCGACCTCCTCAGAGCAGCCGGCTGCCGCCCCGGG
  AAGATGGCGAGGAGGAGCCGCCACCGCCTCCTCCTGCTGCTGCTGCGCTACCTGGTGGTC
  GCCCTGGGCTATCATAAGGCCTATGGGTTTTCTGCCCCAAAAGACCAACAAGTAGTCACA
  GCAGTAGAGTACCAAGAGGCTATTTTAGCCTGCAAAACCCCAAAGAAGACTGTTTCCTCC
  AGATTAGAGTGGAAGAAACTGGGTCGGAGTGTCTCCTTTGTCTACTATCAACAGACTCTT
  CAAGGTGATTTTAAAAATCGAGCTGAGATGATAGATTTCAATATCCGGATCAAAAATGTG
  ACAAGAAGTGATGCGGGGAAATATCGTTGTGAAGTTAGTGCCCCATCTGAGCAAGGCCAA
  AACCTGGAAGAGGATACAGTCACTCTGGAAGTATTAGTGGCTCCAGCAGTTCCATCATGT
  GAAGTACCCTCTTCTGCTCTGAGTGGAACTGTGGTAGAGCTACGATGTCAAGACAAAGAA
  GGGAATCCAGCTCCTGAATACACATGGTTTAAGGATGGCATCCGTTTGCTAGAAAATCCC
  AGACTTGGCTCCCAAAGCACCAACAGCTCATACACAATGAATACANAAACTGGAACTCTG
  CAATTTAATACTGGTTCCCAACTGGACACTGGAGAATATTCCTGTGAAGCCCGCAATTCT
  GTTGGATATCGCAGGTGTCCTGGGAAAACGAATGCAGTAGATGATCTCAACATAAGTGGC
  ATCATAGCAGCCCGTAGTAGTGTGGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021219 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTGCAAA
  GTTGTATTGAAAAGCAATCAATTGGCCCAAACTTAGGTCAAACCTCCAACTAAAAATAAA
  TTAAATCTAGTTACAACAGCAACAATAATTAAAACAAAACAAAGCTTGCTAATAAAATTT
  TAAAGCCCTAATCTTTACACAACACAATTCACTGCAGGAAAATAAAGTCAAAATGCTGTA
  AAAATTACAATGGGATTTTACCAGTTGTTAGGGTTCAAGTTCCTCAAGTAAACACAAATG
  CAAATACAAGTCTGGACACCAGGGACCCACAGATACATATATATACTTTTAATAGGAAAT
  TAGGGCTCAATACTCTGCCCTTTGTGGGGGGGAAAACATTCTTTTATACAAGGATTTTTA
  CCTAGCTATTACAATAGTTTAAGGTAATGTACAATATATATTTGACACAGAGAGTGTTAT
  TAAATGTTCGCACTGCATAAAATGAATCCTCTAGCCTTTGATGTCTTAAAAAGAAGTTTT
  ACAACTATTTAGGGAAGCTAAGGCACTACATATTTTCCTTCCCAATATGGATTGGGGTCA
  TTTAAAACTGAAAAATTGATCTTGTGTGATGACAGGGATGTCTGAAGAGGCGTCTTTCAG
  AGTCATCTATCCTCTTGGCATGCACATGGAAGAGGAAAACTTTTCTCAAACCAAAGATAT
  TTTCAAACCAAAAATAGGGAACTTTAACCCGAATTTTTTAAAAACCTTGTAAAAACGGGT
  TTCTTTTTGTCCTTCACCACTTGAGTTGGGAATTTATGCCCGGGGCCAAATCTGTGCGGG
  GCCTGCGTGTCTTGAAAAAAAGCTTTAGGAAACAAAAACGGAACCTGGCTGTTTGGGTGT
  GGTCGCCTTTGGGCCACAGGGACACCAAATTCCTTCCAGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021219
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021219.2, NP_067042.1
• RefSeq Size: 1721 bp
• RefSeq ORF: 897 bp
• Locus ID: 58494
• UniProt ID: P57087
• Cytogenetics: 21q21.3
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Epithelial cell signaling in Helicobacter pylori infection, Leukocyte transendothelial migration, Tight junction
• Gene Summary: This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.