CAMK2B (NM_001220) Human Untagged Clone

CAT#: SC108187

CAMK2B (untagged)-Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMK2B
• Synonyms: CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001220 edited
  CGCACGCCGAGCCCGTCCGCCGCCGCCATGGCCACCACGGTGACCTGCACCCGCTTCACC
  GACGAGTACCAGCTCTACGAGGATATTGGCAAGGGGGCTTTCTCTGTGGTCCGACGCTGT
  GTCAAGCTCTGCACCGGCCATGAGTATGCAGCCAAGATCATCAACACCAAGAAGCTGTCA
  GCCAGAGATCACCAGAAGCTGGAGAGAGAGGCTCGGATCTGCCGCCTTCTGAAGCATTCC
  AACATCGTGCGTCTCCACGACAGCATCTCCGAGGAGGGCTTCCACTACCTGGTCTTCGAT
  CTGGTCACTGGTGGGGAGCTCTTTGAAGACATTGTGGCGAGAGAGTACTACAGCGAGGCT
  GATGCCAGTCACTGTATCCAGCAGATCCTGGAGGCCGTTCTCCATTGTCACCAAATGGGG
  GTCGTCCACAGAGACCTCAAGCCGGAGAACCTGCTTCTGGCCAGCAAGTGCAAAGGGGCT
  GCAGTGAAGCTGGCAGACTTCGGCCTAGCTATCGAGGTGCAGGGGGACCAGCAGGCATGG
  TTTGGTTTCGCTGGCACACCAGGCTACCTGTCCCCTGAGGTCCTTCGCAAAGAGGCGTAC
  GGCAAGCCCGTGGACATCTGGGCATGTGGGGTGATCCTGTACATCCTGCTCGTGGGCTAC
  CCACCCTTCTGGGACGAGGACCAGCACAAGCTGTACCAGCAGATCAAGGCTGGTGCCTAT
  GACTTCCCGTCCCCTGAGTGGGACACCGTCACTCCTGAAGCCAAAAACCTCATCAACCAG
  ATGCTGACCATCAACCCTGCCAAGCGCATCACAGCCCATGAGGCCCTGAAGCACCCGTGG
  GTCTGCCAACGCTCCACGGTAGCATCCATGATGCACAGACAGGAGACTGTGGAGTGTCTG
  AAAAAGTTCAATGCCAGGAGAAAGCTCAAGGGAGCCATCCTCACCACCATGCTGGCCACA
  CGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCC
  GGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGA
  GTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACG
  CTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATT
  AAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAG
  CAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAG
  GCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAAC
  CTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTGGCCAAGAACAGC
  AAGCCGATCCACACGACCATCCTGAACCCACACGTGCACGTCATTGGAGAGGATGCCGCC
  TGCATCGCTTACATCCGGCTCACGCAGTACATTGACGGGCAGGGCCGGCCCCGCACCAGC
  CAGTCTGAGGAGACCCGCGTGTGGCACCGCCGCGACGGCAAGTGGCAGAACGTGCACTTC
  CACTGCTCGGGCGCGCCTGTGGCCCCGCTGCAGTGAAGAGCTGCGCCCTGGTTTCGCCGG
  ACAGAGTTGGTGTTTGGAGCCCGACTGCCCTCGGGCACACGGCCTGCCTGTCGCATGTTT
  GTGTCTGCCTCGTTCCCTCCCCTGGTGCCTGTGTCTGCAGAAAAACAAGACCAGATGTGA
  TTTGTTAAAAAAAAACAAAAAAAAAAAAAAAAAACAAGATGACGACGACAACCACAAAAA
  AAATTGACATCAGATGAAATGAAAAAAAAAAAAAAAAACT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001220 unedited
  ACATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGCGCAGCCGA
  GCGCACGCCGAGCCCGTCCGCCGCCGCCATGGCCACCACGGTGACCTGCACCCGCTTCAC
  CGACGAGTACCAGCTCTACGAGGATATTGGCAAGGGGGCTTTCTCTGTGGTCCGACGCTG
  TGTCAAGCTCTGCACCGGCCATGAGTATGCAGCCAAGATCATCAACACCAAGAAGCTGTC
  AGCCAGAGATCACCAGAAGCTGGAGAGAGAGGCTCGGATCTGCCGCCTTCTGAAGCATTC
  CAACATCGTGCGTCTCCACGACAGCATCTCCGAGGAGGGCTTCCACTACCTGGTCTTCGA
  TCTGGTCACTGGTGGGGAGCTCTTTGAAGACATTGTGGCGAGAGAGTACTACAGCGAGGC
  TGATGCCAGTCACTGTATCCAGCAGATCCTGGAGGCCGTTCTCCATTGTCACCAAATGGG
  GGTCGTCCACAGAGACCTCAAGCCGGAGAACCTGCTTCTGGCCAGCAAGTGCAAAGGGGC
  TGCAGTGAAGCTGGCAGACTTCGGCCTAGCTATCGAGGTGCAGGGGGACCAGCAGGCATG
  GTTTGGTTTCGCTGGCACACCAGGCTACCTGTCCCCTGAGGTCCTTCGCAAAGAGGCGTA
  CGGCAAGCCCGTGGACATCTGGGCATGTGGGGTGATCCTGTACATCCTGCTCGTGGGCTA
  CCCACCCTTCTGGGACGAGGACCAGCACAAGCTGTACCAGCAGATCAAGGCTGGTGCCTA
  TGACTTCCCGTCCCCTGAGTGGGACACCGTCACTCCTGAAGCCAAAAACTCATCAACAGA
  TGCTGACCATCACCCCTGCCAAGCGCATCACAGCCCATGAGCCCTGAGCACCCGTGGGTC
  TGCCACGCTCCACGTAGCATCCTGATGCCAGACAGAGACTGTGGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001220 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTCATCTGATGTCAATTTTTTTTG
  TGGTTGTCGTCGTCATCTTGTTTTTTTTTTTTTTTTTTGTTTTTTTTTAACAAATCACAT
  CTGGTCTTGTTTTTCTGCAAACACAGGCACCAGGGGAGGGAACGAGGCAAACACAAACAT
  GCGACAGGCAGGCCGTGTGCCCGAGGGCAGTCGGGCTCCAAACACCAACTTTGTCCGGCG
  AAACCAGGGCGCAGCTTTTCACTGCAGCGGGGCCACAGGCGCGCCCGAACAGTGGAAGTG
  CACGTTCTGCCACTTGCCGTCGCGGCGGTGCCACACGCGGGTCTCCTCAAACTGGCTGGT
  GCGGGGCCGGCCCTGCCCGTCAATGTACTGCGTGAGCCGGATGTAAGCGATGCAGGCGGC
  ATCCTCTCCAATGACGTGCACGTGTGGGTTCAGGATGGTCGTGTGGATCGGCTTGCTGTT
  CTTGGCCAACAGGTTCTCGAAGTAAAATCTGTGGAAGTCCATCCCTTCAACCAGGTTGCC
  CAGTGCTTCAAGCTCAAACGAGGTTAGCCCTGGGTACAGATTTTTCGCGTAAGCCTCAAA
  GCCACCGTTGTTGACGGCCTTTATGAGCTGCTCCGCGTTCTTAATGATCTCCTGCTTCCG
  GGCTTTTACCGTTTTATTCTTTATGGTGGAACGGCCCTGTCAGAAGACCCCTAATCCGCC
  CACTGGGTTATAGAGGAACGTGGTCTGAAGCTCCAGGGCCGCAGAAGAAACCCTCTTTTG
  CGGCTGGGGCGGCTGCCCTGTTTTGGGCTTTTCTCCGGGGCTTGAATCCTTCGCTTTTTG
  GTGAAAAACCTTGCTTGTCCACCACCCATGTGGTTCGGAGCTCCGTGGGAATTGCGCCGA
  ACGGGGTTTTTCGCCATAAAAATCCGTGGCCCCGGGGGAAGAAGGTCCTTGACTTTCTGG
  CTAAATTTTAAACACCCACTCCGCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001220
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001220.3, NP_001211.3
• RefSeq Size: 2438 bp
• RefSeq ORF: 2001 bp
• Locus ID: 816
• UniProt ID: Q13554
• Cytogenetics: 7p13
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
• Gene Summary: The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.