BID (NM_197966) Human Untagged Clone

CAT#: SC107715

BID (untagged)-Human BH3 interacting domain death agonist (BID), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BID
• Synonyms: FP497
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_197966 edited
  GAATTCGGCAACGAGGGGCCACCCTTGCCGGCGGATCGGAATCCCCGCCCACACCGTGGT
  CTTTCCAGCACCGCAGACACCTGCCGGCTCCTCCCGAGCGGAGCTCAGGGCTGACAAAGC
  GCGGTCAGAGCGGCCGCTTACTGGGGCTCGCCCGCTCCTTAGAGCACTGGCAATGATGTG
  CGGATCCTCGCTGCTGCTGCTGGGAAACTGTTGAGTGGCTGAATGACCCCAGGGGACCCT
  GGGAGAGCTCTGAAGCCCTCAGCCACCAAGTGGCTGGGCTGGCAAGGGTTCATTCATTCA
  TTCAACAAATACGAATGTGCAGCGGTGCTGGGGTCATGATGGCTCGGTGGGCAGCGAGGG
  GCCGGGCCGGCTGGAGGAGCACAGTGCGGATTCTGTCGCCACTGGGACACTGTGAACCAG
  GAGTGAGTCGGAGCTGCCGCGCTGCCCAGGCCATGGACTGTGAGGTCAACAACGGTTCCA
  GCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTG
  ACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCC
  AGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGG
  GAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACC
  TCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTGG
  CCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTG
  CCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGG
  TGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATG
  TCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAG
  CCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGTGACTGGCTAAAGCTCGATGT
  GGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCC
  TAGAGAAGACAGGCTGGAAGATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTAT
  AACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCAAAAAAAAAAAAAA
  AAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_197966 unedited
  NGGGGAAAATTTGTATACGACTCACTATAGGGGCGGCCGCGAATCGGCAACGAGGGGCCA
  CCCTTGCCGGCGGATCGGAATCCCCGCCCACACCGTGGTCTTTCCAGCACCGCAGACACC
  TGCCGGCTCCTCCCGAGCGGAGCTCAGGGCTGACAAAGCGCGGTCAGAGCGGCCGCTTAC
  TGGGGCTCGCCCGCTCCTTAGAGCACTGGCAATGATGTGCGGATCCTCGCTGCTGCTGCT
  GGGAAACTGTTGAGTGGCTGAATGACCCCAGGGGACCCTGGGAGAGCTCTGAAGCCCTCA
  GCCACCAAGTGGCTGGGCTGGCAAGGGTTCATTCATTCATTCAACAAATACGAATGTGCA
  GCGGTGCTGGGGTCATGATGGCTCGGTGGGCAGCGAGGGGCCGGGCCGGCTGGAGGAGCA
  CAGTGCGGATTCTGTCGCCACTGGGACACTGTGAACCAGGAGTGAGTCGGAGCTGCCGCG
  CTGCCCAGGCCATGGACTGTGAGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCA
  CAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGC
  TGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGC
  TGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGAAGATAGAGGCAGATTCTTG
  AAGTCAAGAAGACCTCATCCGGAATATTGCCCAGCACCCTCGCCCAGGTCGGGGACAGCA
  TGGACCCGGACATCCCCTCCGGGCCCGGTGGACCGGGCTGCCCTGGCGCTTAAGAACACC
  ACCCCGGCCGGAGGGAGACCGGAACACGGGACCTGCCCACTGCCCTGGGACACCTGCTGC
  AGGCCTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_197966 unedited
  NTTTTACTCTGGNACCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGACA
  TTTTCATTCAAGTATATTAATGTAGATATTTTAAAGTGGGTTATAAGTTTAACATTGTCT
  TTAAAATAGAAGTCACAGCTATCTTCCAGCCTGTCTTCTCTAGGAACGCTGTTGACATGC
  CAGGGCTCCGTCTACACTGGAAGCAGCTATACAGCTGTGACCACATCGAGCTTTAGCCAG
  TCACACTTCTGGAACTGTCCGTTCAGTCCATCCCATTTCTGGCTAAGCTCCTCACGTAGG
  TGCGTAGGTTCTGGTTAATAAAATTCACTGTTGTGTGAAAGACATCACGGAGCAAGGACG
  GCGTGTGACTGGCCACCTTCTTGGCCAGCAGCAGGGCCAGCACCAGCATGGTCTTCTCCT
  TCTCCATGTCTCTAGGGTAGGCCTGCAGCAGCTGCTCCAGGGCAGTGGCCAGGTCCCTGT
  TCCGGTCCTCCTCCGACCGGCTGGTGTTCCTGAGCTGCAGGGCCAGGCCGTTCACCAGGC
  CCGGAGGGATGCTACGGTCCATGCTGTCCCCGACCTGGGCGAGGTGCCTGGCAATATTCC
  GGATGATGTCTTCTTGACTTTCAGAATCTGCCTCTATTCTTCCCAAGCGGGAGTGGCTGC
  TGCGGTTGCCATCAGTCTGCAGCTCATCGTAGCCCTCCCACTGGGGAGCCAGCACTGGCA
  GCTCGTGGCCCAGTGCGTCCAGCTCTCTGCGGAAGCTGTTGTCAGAACAGCTTTGGAGGA
  AGCAAACCCAGTAGGGTTTGTGATGCACTCATCCCTGGAGCTGAAACCGGTTGGTGACCT
  CCCAGGTCATGGCCTGGGCAGCGCCGCAGCCTCGACTCACTCTGGGTCACAGGGTCCCCA
  GGGGCGACAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_197966
• Insert Size: 1660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_197966.1, NP_932070.1
• RefSeq Size: 2506 bp
• RefSeq ORF: 726 bp
• Locus ID: 637
• UniProt ID: P55957
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Pathways in cancer, Viral myocarditis
• Gene Summary: This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).