PAK5 (NM_177990) Human Untagged Clone

CAT#: SC107077

PAK7 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK5
• Synonyms: PAK7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_177990 edited
  ATGTTTGGGAAGAAAAAGAAAAAGATTGAAATATCTGGCCCGTCCAACTTTGAACACAGG
  GTTCATACTGGGTTTGATCCACAAGAGCAGAAGTTTACCGGCCTTCCCCAGCAGTGGCAC
  AGCCTGTTAGCAGATACGGCCAACAGGCCAAAGCCTATGGTGGACCCTTCATGCATCACA
  CCCATCCAGCTGGCTCCTATGAAGACAATCGTTAGAGGAAACAAACCCTGCAAGGAAACC
  TCCATCAACGGCCTGCTAGAGGATTTTGACAACATCTCGGTGACTCGCTCCAACTCCCTA
  AGGAAAGAAAGCCCACCCACCCCAGATCAGGGAGCCTCCAGCCACGGTCCAGGCCACGCG
  GAAGAAAATGGCTTCATCACCTTCTCCCAGTATTCCAGCGAATCCGATACTACTGCTGAC
  TACACGACCGAAAAGTACAGGGAGAAGAGTCTCTATGGAGATGATCTGGATCCGTATTAT
  AGAGGCAGCCACGCAGCCAAGCAAAATGGGCACGTAATGAAAATGAAGCACGGGGAGGCC
  TACTATTCTGAGGTGAAGCCTTTGAAATCCGATTTTGCCAGATTTTCTGCCGATTATCAC
  TCACATTTGGACTCACTGAGCAAACCAAGTGAATACAGTGACCTCAAGTGGGAGTATCAG
  AGAGCCTCGAGTAGCTCCCCTCTGGATTATTCATTCCAATTCACACCTTCTAGAACTGCA
  GGGACCAGCGGGTGCTCCAAGGAGAGCCTGGCGTACAGTGAAAGTGAATGGGGACCCAGC
  CTGGATGACTATGACAGGAGGCCAAAGTCTTCGTACCTGAATCAGACAAGCCCTCAGCCC
  ACCATGCGGCAGAGGTCCAGGTCAGGCTCGGGACTCCAGGAACCGATGATGCCATTTGGA
  GCAAGTGCATTTAAAACCCATCCCCAAGGACACTCCTACAACTCCTACACCTACCCTCGC
  TTGTCCGAGCCCACAATGTGCATTCCAAAGGTGGATTACGATCGAGCACAGATGGTCCTC
  AGCCCTCCACTGTCAGGGTCTGACACCTACCCCAGGGGCCCTGCCAAACTACCTCAAAGT
  CAAAGCAAATCGGGCTATTCCTCAAGCAGTCACCAGTACCCGTCTGGGTACCACAAAGCC
  ACCTTGTACCATCACCCCTCCCTGCAGAGCAGTTCGCAGTACATCTCCACGGCTTCCTAC
  CTGAGCTCCCTCAGCCTCTCATCCAGCACCTACCCGCCGCCCAGCTGGGGCTCCTCCTCC
  GACCAGCAGCCCTCCAGGGTGTCCCATGAACAGTTTCGGGCGGCCCTGCAGCTGGTGGTC
  AGCCCAGGAGACCCCAGGGAATACTTGGCCAACTTTATCAAAATCGGGGAAGGCTCAACC
  GGCATCGTATGCATCGCCACCGAGAAACACACAGGGAAACAAGTTGCAGTGAAGAAAATG
  GACCTCCGGAAGCAACAGAGACGAGAATTGCTTTTCAATGAGGTCGTGATCATGCGGGAT
  TACCACCATGACAATGTGGTTGACATGTACAACAGCTACCTTGTCGGCGATGAGCTCTGG
  GTGGTCATGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTGACTCACACCAGAATG
  AATGAAGAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAGAGCTCTCTCCTACCTTCAT
  AACCAAGGAGTGATTCACAGGGACATAAAAAGTGACTCCATCCTCCTGACAAGCGATGGC
  CGGATAAAGTTGTCTGATTTTGGTTTCTGTGCTCAAGTTTCCAAAGAGGTGCCGAAGAGG
  AAATCATTGGTTGGCACTCCCTACTGGATGGCCCCTGAGGTGATTTCTAGGCTACCTTAT
  GGGACAGAGGTGGACATCTGGTCCCTCGGGATCATGGTGATAGAAATGATTGATGGCGAG
  CCCCCCTACTTCAATGAGCCTCCCCTCCAGGCGATGCGGAGGATCCGGGACAGTTTACCT
  CCAAGAGTGAAGGACCTACACAAGGTTTCTTCAGTGCTCCGGGGATTCCTAGACTTGATG
  TTGGTGAGGGAGCCCTCTCAGAGAGCAACAGCCCAGGAACTCCTCGGACATCCATTCTTA
  AAACTAGCAGGTCCACCGTCTTGCATCGTCCCCCTCATGAGACAATACAGGCATCACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177990 unedited
  AATCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACCGTGGGAGGTCTATATAAGCAG
  AGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAT
  TCGGCACGAGGCCCCCTTGCCCCTACCCAGCCAGTAGTAGTTCCCCAGCGTGCGCCCGGG
  GAGACCGGGAACATGGCGCTGGGAGCGCTGTAGCATCTGAGAAGGGGCTGAGGCACCGCC
  GCTTCGCTGACAGCCGGCCACCAGAAATACACTGTGGTGAAATGCTTCCACCTCTTGCTA
  AAATGAACACTGAGAAAAAATGAAGAAGACTGACAAGCACCAGCGAAAAGTTGCAAAATA
  CAAATAGCCACACTCCTCTGGAGTCTTTAATTCATCCACAGCCATCATATAAAGGTTTTG
  GCATCATGTTTGGGAAGAAAAAGAAAAAGATTGAAATATCTGGCCCGTCCAACTTTGAAC
  ACAGGGTTCATACTGGGTTTGATCCACAAGAGCAGAAGTTTACCGGCCTTCCCCAGCAGT
  GGCACAGCCTGTTAGCAGATACCGTCAACATGCCAAAGCCTATGGTGGACCCTTCATGCA
  TCACACCCATCCAGCTGGCTCCTATGAAGACAATCGTTAGAGGAAACAAACCCTGCAAGG
  AAACCTCCATGAACGGCCTGCTAGAGGATTTTGACAACATCTTAGTGACTCGCTGCAACT
  CCATAGGTAAGAAAGCCCACCCACCCCACATCAGGGAGCCTCTCGCCACGGTCCAGGCCA
  CGCCGGAACATATGGGCTTCATACCTTCTCCCAGTATTCCAGCGATCCCATACTACTGGT
  GACTACACGACCGAATATGCCGGGAGAACATCCTCTTTGAAATGAACTGCATCCGTTTAT
  AGACGCATCCGCGCAGCCAGGCAATGTGCCCTGTAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_177990 unedited
  ATTAGATTTCTTTCATATANAAAATCTACTGTTACCCTGCATGTGGCTAGGATATATCAT
  AACGGAGTTTGTACTGAGTCCTTCTGATTTGCTGGATGAAGGGCTGAAAAATATAATAAT
  GACTTATTAAAGCCGTGCTCCAAGTGACCACACCGGCTGTCAGTGGAGAGATGCCTGTTT
  AAGACACAAGAAGATGCCCTGGTCTGTTGAACCCTGCCGGTCATCACGCTGTCCCACCAA
  TTGGCTGGTCTAGAATGCACAGGCCTTTTGCATGTTCTGTGTTTCCTTTTGTTCTCCTGA
  ATTATTCTCATGTCCTCATCTAGCTTTGCCACCTACACGAATCCTCTGCTCAGTGATGCC
  TGTATTGTCTCATGAGGGGGACGATGCAAGACGGTGGACCTGCTAGTTTTAAGAATGGAT
  GTCCGAGGAGTTCCTGGGCTGTTGCTCTCTGAGAGGGCTCCCTCACCAACATCAAGTCTA
  GGAATCCCCGGAGCACTGAAGAAACCTTGTGTAGGTCCTTCACTCTTGGAGGTAAACTGT
  CCCGGATCCTCCGCATCGCCTGGAGGGGAGGCTCATTGAAGTAGGGGGGCTCGCCATCAA
  TCATTTCTATCACCATGATCCCGAGGGACCAGATGTCCACCTCTGTCCCATAAGGTAGCC
  TAGAAATCACCTCAGGGGCCATCCAGTANGGAGTGCCAACCAATGATTTCCCTCTTCGCA
  CCTCTTTGGGAACTTGAGCACAGAAACCAAATCAGACACTTTTTCCGGCCATCGCTTGGT
  CAGGAGATGGAGTCACTTTTTATGTCCCTGTGGATCACTCCCTTGTTATGAAGGTAGGGA
  GAGAGCTCTTAGAACTGACAGGCAGACAGGAGCTATCTGGTCTTCTTCATTCTGGTGTGA
  GTCACATGTCTGTCAGGCACCCACTTCTAAAACTCCTGAACCCCAGACTCATGCCGAAAG
  TAGCTGTGTCCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177990
• Insert Size: 4840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177990.1, NP_817127.1
• RefSeq Size: 4506 bp
• RefSeq ORF: 2160 bp
• Locus ID: 57144
• UniProt ID: Q9P286
• Cytogenetics: 20p12.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an internal 5' UTR exon, as compared to variant 1. Both variants encode the same protein.