Apc11 (ANAPC11) (BC000607) Human Untagged Clone

CAT#: SC105984

ANAPC11 (untagged)-Homo sapiens, clone MGC:882 IMAGE:3346039, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANAPC11
• Synonyms: anaphase promoting complex subunit 11; anaphase promoting complex subunit 11 (yeast APC11 homolog); APC11; APC11 anaphase promoting complex subunit 11; APC11 anaphase promoting complex subunit 11 homolog; Apc11p; HSPC214; MGC882
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC000607, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGGTGAAGATTAAGTGCTGGAACGGCGTGGCCACTTGGCTCTGGGTGGCCAACGATGAGAACTGTG
  GCATCTGCAGGATGGCATTTAACGGATGCTGCCCTGACTGTCCTCTCCATGGAGAAAGCATTTCTAGGTG
  TTTGGGCTGGTGCCCGCAGCCTGTGCCTGTCCTGGGAGGCAGGGCCCATCCACAGGTGCCCATCAACACA
  GCTTCCCCAACGCCTGGGCAGCACACCGGATCCCTGATGTCTAGGGAAGAGTCTTCTAGGTCCCCAGACC
  CCACCCCTCCTGCCCTTGATCAAGAGACCAGTTCACTACTCAGATGCACGTCTCCTTGGTGCCTTGACCA
  TTCATGTGACCTTTTTGGCATCACAGATCAAGTGTCTGCAGATGGGCCCAGGGCCTGTAGGCAAGGTGCC
  CGGCGACGACTGCCCGCTGGTGTGGGGCCAGTGCTCCCACTGCTTCCACATGCATTGCATCCTCAAGTGG
  CTGCACGCACAGCAGGTGCAGCAGCACTGCCCCATGTGCCGCCAGGAATGGAAGTTCAAGGAGTGAGGCC
  CGACCTGGCTCTCGCTGGAGGGGCATCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC000607 unedited
  TTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGTCGGGCCGCGACTGTG
  GTCGTTTTTATACCTTCCCGCGCGGACGCCGGCGCTGCCAACGGAAGGGCGGGTAGGGCG
  GCTCTGCTGCCATGAAGGTGAAGATTAAGTGCTGGAACGGCGTGGCCACTTGGCTCTGGG
  TGGCCAACGATGAGAACTGTGGCATCTGCAGGATGGCATTTAACGGATGCTGCCCTGACT
  GCAAGGTGCCCGGCGACGACTGCCCGCTGGTGTGGGGCCAGTGCTCCCACTGCTTCCACA
  TGCATTGCATCCTCAAGTGGCTGCACGCACAGCAGGTGCAGCAGCACTGCCCCATGTGCC
  GCCAGGAATGGAAGTTCAAGGAGTGAGGCCCGACCTGGCTCTCGCTGGAGGGGCATCCTG
  AGACTCCTTCCTCATGCTGGCGCCGATGGCTGCTGGGGACAGCGCCCCTGAGCTGCAACA
  AGGTGGAAACAAGGGCTGGAGCTGCGTTTGTTTTGCCATCACTATGTTGACACTTTTATC
  CAATAAGTGAAAACTCATTAAACTACTCAAATCTTGAAAAANAAAAAAAAAAAACTCGAC
  TCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTG
  ACCCCTCCNCAGTGCCTCTCCTGGCCCTGNAAGTTGCCACTCCAGTGCCCACCAGCCTTG
  TCCTATAAAATTAAGTTGATCATTTTGTCTGACTAGGTGTCCTTCTATATATATGGGGAG
  GNNGGGGGGCGGTTGGAGACACAGGCGCAATTTGGGAAAAAACACTGTGGGCCTGGTGGG
  TTCATTGGGGAACAGCCGGCGTGGCGGGGACAATTTTGGCTTATTGCAACCTCCCTCCCT
  GGGTTCAGCGATTTCCTGCTCACCTCCGGAGTGTGGGATCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC000607 unedited
  NNNCCGGTATTACTATGNNACCGCGGCCGCAANCTANGATCGGATTTTTTTTTTTTTTTT
  TTCAAGATTGAGTAGTTTAATGAGTTTTCACTTATTGGATAAAAGTGTCAACATAGTGAT
  GGCAAAACAAACGCAGCTCCAGCCCTTGTTTCCACCTTGTTGCAGCTCAGGGGCGCTGTC
  CCCAGCAGCCATCGGCGCCAGCATGAGGAAGGAGTCTCAGGATGCCCCTCCAGCGAGAGC
  CAGGTCGGGCCTCACTCCTTGAACTTCCATTCCTGGCGGCACATGGGGCAGTGCTGCTGC
  ACCTGCTGTGCGTGCAGCCACTTGAGGATGCAATGCATGTGGAAGCAGTGGGAGCACTGG
  CCCCACACCAGCGGGCAGTCGTCGCCGGGCACCTTGCAGTCAGGGCAGCATCCGTTAAAT
  GCCATCCTGCAGATGCCACAGTTCTCATCGTTGGCCACCCAGAGCCAAGTGGCCACGCCG
  TTCCAGCACTTAATCTTCACCTTCATGGCAGCAGAGCCGCCCTACCCGCCCTTCCGTTGG
  CAGCGCCGGCGTCCGCGCGGGAAGGTATAAAAACGACCACAGTCGCGGCCCGACCCTCGT
  GCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAAC
  GAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGG
  CGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCAT
  TGACGTCAATGGGGTGGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCA
  TTGGNTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTA
  
• Restriction Sites: NotI-NotI
• ACCN: BC000607
• Insert Size: 580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC000607.1, AAH00607.2
• RefSeq Size: 901 bp
• RefSeq ORF: 591 bp
• Locus ID: 51529
• Cytogenetics: 17q25.3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.[UniProtKB/Swiss-Prot Function]