CDC23 (BC010944) Human Untagged Clone

CAT#: SC105953

CDC23 (untagged)-Homo sapiens, clone MGC:13585 IMAGE:4281800, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC23
• Synonyms: anaphase-promoting complex subunit 8; APC8; cell division cycle 23 homolog (S. cerevisiae); cell division cycle protein 23
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC010944, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTGTCCATAAACAGCGATTTCTCAGATTTGC
  GGGAAATTAAAAAGCAACTGCTGCTTATTGCGGGCCTTACCCGGGAGCGGGGCCTACTACACAGTAGCAA
  ATGGTCGGCGGAGTTGGCTTTCTCTCTCCCTGCATTGCCTCTGGCCGAGCTGCAACCGCCTCCGCCTATT
  ACAGAGGAAGATGCCCAGGATATGGATGCCTATACCCTGGCCAAGGCCTACTTTGACGTTAAAGAGTATG
  ATCGGGCAGCACATTTCCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCTGTATATGTATTCCAGATA
  TCTGGTGAGGGCCATTTTAAAATGTCATTCTGCCTTTAGTGAAACATCCATATTTAGAACCAATGGAAAA
  GTTAAATCTTTTAAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC010944 unedited
  NGGGTCACATTGATACACTATTAGGGGCCGGATTCGCCGAGGGAAAATGGCTGCGAGTAC
  CTCCATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTGTCCATAAACAGCGA
  TTTCTCAGATTTGCGGGAAATTAAAAAGCAACTGCTGCTTATTGCGGGCCTTACCCGGGA
  GCGGGGCCTACTACACAGTAGCAAATGGTCGGCGGAGTTGGCTTTCTCTCTCCCTGCATT
  GCCTCTGGCCGAGCTGCAACCGCCTCCGCCTATTACAGAGGAAGATGCCCAGGATATGGA
  TGCCTATACCCTGGCCAAGGCCTACTTTGACGTTAAAGAGTATGATCGGGCAGCACATTT
  CCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCTGTATATGTATTCCAGATATCTGTC
  TGGAGAAAAAAAGAAGGACGATGAAACAGTTGATAGCTTAGGCCCCCTGGAAAAAGGACA
  AGTGAAAAATGAGGCGCTTAGAGAATTGAGAGTGGAGCTCAGCAAAAAACACCAAGCTCG
  AGAACTTGATGGATTTGGACTTTATCTGTATGGTGTGGTGCTTCGAAAACTGGACTTGGT
  TAAAGAGGCCATTGATGTGTTTGTGGAAGCTACTCATGTTTTGCCCTTGCATTGNGGAGC
  CTGGTTAGAACTCTGTAACCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCC
  AGACACCTGGATGAAAGAAGTTTTTCTGGCTCATATATACACAGAGTTGCAGTTGATAGA
  GGAGGCCCTGCAAAGTATCAGAACTCATTGATGTGGGCTTTCTCTAGAGCTCGTATATTG
  TTTCCCAAATTGCAGTTGCCTATCACATATCAGAGATATTGACCAAGCCCTCTCCATTTT
  TATGAN
  
• Restriction Sites: NotI-NotI
• ACCN: BC010944
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010944.1, AAH10944.1
• RefSeq Size: 1088 bp
• RefSeq ORF: 438 bp
• Locus ID: 8697
• Cytogenetics: 5q31.2
• Domains: APC8
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]