S6K1 (RPS6KB1) (NM_003161) Human Untagged Clone

CAT#: SC105680

RPS6KB1 (untagged)-Human ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: S6K1
• Synonyms: p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003161 edited
  ATGAGGCGACGAAGGAGGCGGGACGGCTTTTACCCAGCCCCGGACTTCCGAGACAGGGAA
  GCTGAGGACATGGCAGGAGTGTTTGACATAGACCTGGACCAGCCAGAGGACGCGGGCTCT
  GAGGATGAGCTGGAGGAGGGGGGTCAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGA
  CCATATGAACTTGGCATGGAACATTGTGAGAAATTTGAAATCTCAGAAACTAGTGTGAAC
  AGAGGGCCAGAAAAAATCAGACCAGAATGTTTTGAGCTACTTCGGGTACTTGGTAAAGGG
  GGCTATGGAAAGGTTTTTCAAGTACGAAAAGTAACAGGAGCAAATACTGGGAAAATATTT
  GCCATGAAGGTGCTTAAAAAGGCAATGATAGTAAGAAATGCTAAAGATACAGCTCATACA
  AAAGCAGAACGGAATATTCTGGAGGAAGTAAAGCATCCCTTCATCGTGGATTTAATTTAT
  GCCTTTCAGACTGGTGGAAAACTCTACCTCATCCTTGAGTATCTCAGTGGAGGAGAACTA
  TTTATGCAGTTAGAAAGAGAGGGAATATTTATGGAAGACACTGCCTGCTTTTACTTGGCA
  GAAATCTCCATGGCTTTGGGGCATTTACATCAAAAGGGGATCATCTACAGAGACCTGAAG
  CCGGAGAATATCATGCTTAATCACCAAGGTCATGTGAAACTAACAGACTTTGGACTATGC
  AAAGAATCTATTCATGATGGAACAGTCACACACACATTTTGTGGAACAATAGAATACATG
  GCCCCTGAAATCTTGATGAGAAGTGGCCACAATCGTGCTGTGGATTGGTGGAGTTTGGGA
  GCATTAATGTATGACATGCTGACTGGAGCACCCCCATTCACTGGGGAGAATAGAAAGAAA
  ACAATTGACAAAATCCTCAAATGTAAACTCAATTTGCCTCCCTACCTCACACAAGAAGCC
  AGAGATCTGCTTAAAAAGCTGCTGAAAAGAAATGCTGCTTCTCGTCTGGGAGCTGGTCCT
  GGGGACGCTGGAGAAGTTCAAGCTCATCCATTCTTTAGACACATTAACTGGGAAGAACTT
  CTGGCTCGAAAGGTGGAGCCCCCCTTTAAACCTCTGTTGCAATCTGAAGAGGATGTAAGT
  CAGTTTGATTCCAAGTTTACACGTCAGACACCTGTCGACAGCCCAGATGACTCAACTCTC
  AGTGAAAGTGCCAATCAGGTCTTTCTGGGTTTTACATATGTGGCTCCATCTGTACTTGAA
  AGTGTGAAAGAAAAGTTTTCCTTTGAACCAAAAATCCGATCACCTCGAAGATTTATTGGC
  AGCCCACGAACACCTGTCAGCCCAGTCAAATTTTCTCCTGGGGATTTCTGGGGAAGAGGT
  GCTTCGGCCAGCACAGCAAATCCTCAGACACCTGTGGAATACCCAATGGAAACAAGTGGC
  ATAGAGCAGATGGATGTGACAATGAGTGGGGAAGCATCGGCACCACTTCCAATACGACAG
  CCGAACTCTGGGCCATACAAAAAACAAGCTTTTCCCATGATCTCCAAACGGCCAGAGCAC
  CTGCGTATGAATCTATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003161 unedited
  NGTCGGTTTTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGGTGATGG
  CGGCAGCGGCTGTGGTGGCTGCGGCGGGTCCGGGCCCATGAGGCGACGAAGGAGGCGGGA
  CGGCTTTTACCCAGCCCCGGACTTCCGAGACAGGGAAGCTGAGGACATGGCAGGAGTGTT
  TGACATAGACCTGGACCAGCCAGAGGACGCGGGCTCTGAGGATGAGCTGGAGGAGGGGGG
  TCAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGAACTTGGCATGGAACA
  TTGTGAGAAATTTGAAATCTCAGAAACTAGTGTGAACAGAGGGCCAGAAAAAATCAGACC
  AGAATGTTTTGAGCTACTTCGGGTACTTGGTAAAGGGGGCTATGGAAAGGTTTTTCAAGT
  ACGAAAAGTAACAGGAGCAAATACTGGGAAAATATTTGCCATGAAGGTGCTTAAAAAGGC
  AATGATAGTAAGAAATGCTAAAGATACAGCTCATACAAAAGCAGAACGGAATATTCTGGA
  GGAAGTAAAGCATCCCTTCATCGTGGATTTAATTTATGCCTTTCAGACTGGTGGAAAACT
  CTACCTCATCCTTGAGTATCTCAGTGGAGGAGAACTATTTATGCAGTTAGAAAGAGAGGG
  AATATTTATGGAAGACACTGCCTGCTTTTACTTGGCAGAAATCTCCATGGCTTTGGGGCA
  TTTACATCANAAGGGGATCATCTACAGAGACCTGAAGCCGGAGAATATCATGCTTAATCA
  CCAAGGTCATGTGAAACTAACAGACTTTGGACTATGCAAAGAATCTATTCATGATGGGAA
  CAGTCACACACCCATTTNTGTGGAAACATAGAATACATGGCCCCTGAAATCTGATGAGAA
  GTGCCACATCGTCTGGGATGNGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003161 unedited
  NTTTTAGCTTGGNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTCATTACAAATTGGCATTTAATTTCTATTTACATTCATTCAATCCGAAATCAAATAAT
  ACAGTCAACTAAATACAAGATGAAAAGCAGAACAGGGGTTACCCTCATAGCACTCCTAAT
  AAGGCAGCTGAAAAATTTTATTTACTGATAGATGCAAAGCGAACTTGGGATAGGAGAAAC
  AATAGTTTAAAGAGCAAACACAAACATAGATGAACCCATCTTTCAAATGTTATTCCGGAT
  AATCCACTGAGATAATACTTGGGCTATAATGTTAAAAATCACACAAGAAAAAGTCCTGTA
  TTAATCAGCAACAGACCACAGGTTATGTGTGACAAATACTTTTGGGATAGTTTGCCCAGC
  TTGCTAGGGTCTACTCCTATGAAAAAAGGTAAATGTTTGGACATTGATGTTTTTGAGAAA
  TTAACCATTTTCAAAGCCTTAAAATCTATTCTTCATAAATTCAACATTTCTCATGGGAAT
  AATCTTAGATCTAGCCCTTGTAAGCTAGGAACCATATGATTAACTGAATATTCAAAATAG
  GTCCTATATTAAATATATAATCCAAGGCTCAGTATATTAATACTATCTAAGATGCATCTT
  GTTTATAATCATTCATTACCTATAGTAAAGAATGTTGAATTTATGGAGTATCATACATGC
  CTCCTCCACCCCTGCCCACAAATTATCTTTTATCTTAAAAAAAGTATGGAAAATCCTTCT
  TGAGGATTCAATTAGTTAAGACCTAAAGGGGGAAAAGAACTAATTTTCCCTAAAATCGGG
  TCAAAGTTCAATAATAAAACTTTACATGCTAATCACAGCTGGTTTATAAACCCAGCTAAG
  TATTCTGGATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003161
• Insert Size: 5100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003161.2, NP_003152.1
• RefSeq Size: 5332 bp
• RefSeq ORF: 1578 bp
• Locus ID: 6198
• UniProt ID: P23443
• Cytogenetics: 17q23.1
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway
• Gene Summary: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (1) can initiate translation from two alternate in-frame AUG start codons. The isoform represented in this RefSeq (a, also known as p85 or alpha I) is derived from the first AUG start codon, and is the longest isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.