FADS1 (BD127299) Human Untagged Clone

CAT#: SC105368

(untagged)-Primer for synthesizing full-length cDNA and use thereof

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FADS1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BD127299, the custom clone sequence may differ by one or more nucleotides
  
  
  GTGAGTTGCCCAAGACCCACCGGGGGACGGGATCTCGCTCCCCGCGCCACGAGGCTCGGCCAATGGGAAC
  GCGCGCTGCGAGGCCCGCCGGTCTGCCCTGCGGTGCTGAAAACCCGGCGCGCAGGCGGCTGGCTCTGGGC
  GCGCGCCAGCAAATCCACTCCTGGAGCCCGCGGACCCCGAGCACGCGCCTGACAGCCCCTGCTGGCCCGG
  CGCGCGGCGTCGCCAGGCCAGCTATGGCCCCCGACCCGGTGGCCGCCGAGACCGCGGCTCAGGGACCTAC
  CCCGCGCTACTTCACCTGGGACGAGGTGGCCCAGCGCTCAGGGTGCGAGGAGCGGTGGCTAGTGATCGAC
  CGTAAGGTGTACAACATCAGCGAGTTCACCCGCCGGCATCCAGGGGGCTCCCGGGTCATCAGCCACTACG
  CCGGGCAGGATGCCACGGATCCCTTTGTGGCCTTCCACATCAACAAGGGCCTTGTGAAGAAGTATATGAA
  CTCTCTCCTGATTGGAGAACTGTCTCCAGAGCAGCCCAGCTTTGAGCCCACCAAGAATAAAGAGCTGACA
  GATGAGTTCCGGGAGCTGCGGGCCACAGTGGAGCGGATGGGGCTCATGAAGGCCAACCATGTCTTCTTCC
  TGCTGTACCTGCTGCACATCTTGCTGCTGGATGGTGCAGCCTGGCTCACCCTTTGGGTCTTTGGGACGTC
  CTTTTTGCCCTTCCTCCTCTGTGCGGTGCTGCTCAGTGCAGTTCAGGCCCAGGCTGGCTGGCTGCAGCAT
  GACTTTGGGCACCTGTCGGTCTTCAGCACCTCAAAGTGGAACCATCTGCTACATCATTTTGTGATTGGCC
  ACCTGAAGGGGGCCCCCGCCAGTTGGTGGAACCACATGCACTTCCAGCACCATGCCAAGCCCAACTGCTT
  CCGCAAAGACCCAGACATCAACATGCATCCCTTCTTCTTTGCCTTGGGGAAGATCCTCTCTGTGGAGCTT
  GGGAAACAGAAGAAAAAATATATGCCGTACAACCACCAGCACAAATACTTCTTCCTAATTGGGCCCCCAG
  CCTTGCTGCCTCTCTACTTCCAGTGGTATATTTTCTATTTTGTTATCCAGCGAAAGAAGTGGGTGGACTT
  GGCCTGGATGATTACCTTCTACGTCCGCTTCTTCCTCACTTATGTGCCACTATTGGGGCTGAAAGCCTTC
  CTGGGCCTTTTCTTCATAGTCAGGTTCCTGGAAAGCAACTGGTTTGTGTGGGTGACACAGATGAACCATA
  TTCCCATGCACATTGATCATGACCGGAACATGGACTGGGTTTCCACCCAGCTCCAGGCCACATGCAATGT
  CCACAAGTCTGCCTTCAATGACTGGTTCAGTGGACACCTCAACTCCCAGATTGAGCACCATCTTTTTCCC
  ACGATGCCTCGACACAATTACCACAAAGTGGCTCCCCTGGTGCAGTCCTTGTGTGCCAAGCATGGCATAG
  AGTACCAGTCCAAGCCCCTGCTGTCAGCCTTCGCCGACATCATCCACTCACTAGAGGAGTCAGGGCAGCT
  CTGGCTAGATGCCTATCTTCACCAATAACAACAGCCACCCTGCCCAGTCTGGAAGAAGAGGAGGAAGACT
  CTGGAGCCAAGGCAGAGGGGAGCTTGAGGGACAATGCCACTATAGTTTAATACTCAGAGGGGGTTGGGTT
  TGGGGACATAAAGCCTCTGACTCAAACTCCTCCCTTTTATCTTCTAGCCACAGTTCTAAGACCCAAAGTG
  GGGGGTGGACACAGAAGTCCCTAGGAGGGAAGGAGCTGTTGGGGCAGGGGTGTAAATTATTTCCTTTTTC
  TAGTTTGGCACATGCAGGTAGTTGGTGAACAGAGAGAACCAGGAGGGTAACAGAAGAGGAGGGACCTACT
  GAACCCAGAGTCAGGAAGAGATTTAACACTAAAATTCCACTCATGCCGGGCGTGGTGGCACGCGCCTGTA
  ATCCCAGCTACCCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCGGGGAGGTGGAGGTTGCAGTGAGCTG
  AGATCACGCCATTGTACTCCAGCCTGGGCGACAGAGCAAGACTCCATTTC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BD127299 unedited
  TCTATAACCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG
  CGAATTCGGCACGAGGGTTGCCCAAGACCCACCGGGGGACGGGATCTCGCTCCCCGCGCC
  ACGAGGCTCGGCCAATGGGAACGCGCGCTGCGAGGCCCGCCGGTCTGCCCTGCGGTGCTG
  AAAACCCGGCGCGCAGGCGGCTGGCTCTGGGCGCGCGCCAGCAAATCCACTCCTGGAGCC
  CGCGGACCCCGAGCACGCGCCTGACAGCCCCTGCTGGCCCGGCGCGCGGCGTCGCCAGGC
  CAGCTATGGCCCCCGACCCGGTGGCCGCCGAGACCGCGGCTCAGGGACCTACCCCGCGCT
  ACTTCACCTGGGACGAGGTGGCCCAGCGCTCAGGGTGCGAGGAGCGGTGGCTAGTGATCG
  ACCGTAAGGTGTACAACATCAGCGAGTTCACCCGCCGGCATCCAGGGGGCTCCCGGGTCA
  TCAGCCACTACGCCGGGCAGGATGCCACGGATCCCTTTGTGGCCTTCCACATCAACAAGG
  GCCTTGTGAAGAAGTATATGAACTCTCTCCTGATTGGAGAACTGTCTCCAGAGCAGCCCA
  GCTTTGAGCCCACCAAGAATAAAGAGCTGACAGATGAGTTCCGGGAGCTGCGGGCCACAG
  TGGAGCGGATGGGGCTCATGAAGGCCAACCATGTCTTCTTCCTGCTGTACCTGCTGCACA
  TCTTGCTGCTGGATGGTGCAGCCTGGCTCACCCTTTGGGTCTTTGGGACGTCCTTTTTGC
  CCTTCCTCCTCTGTGCCGTGCTGCTCAGTGCAGTTCAGGCCCCAGCTGGCTGGCTGCAGC
  ATGACTTTGGGCACCCTGTCGTCTTCAGCACTCAAGTGGAACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BD127299 unedited
  TATGGCCGCGGCCGCATCTAAATCGAGTTTTTTTTTTTTTTTTTTGAAATGGAGTCTTGC
  TCTGTCGCCCAGGCTGGAGTACAATGGCGTGATCTCAGCTCACTGCAACCTCCACCTCCC
  CGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGGGTAGCTGGGATTACAGGCGCGTGCCA
  CCACGCCCGGCATGAGTGGAATTTTAGTGTTAAATCTCTTCCTGACTCTGGGTTCAGTAG
  GTCCCTCCTCTTCTGTTACCCTCCTGGTTCTCTCTGTTCACCAACTACCTGCATGTGCCA
  AACTAGAAAAAGGAAATAATTTACACCCCTGCCCCAACAGCTCCTTCCCTCCTAGGGACT
  TCTGTGTCCACCCCCCACTTTGGGTCTTAAAACTGTGGCTAGAAGATAAAAGGGAGGAGT
  CCGAGTCAGAGGCTTTATGTCCCCAAACCCAACCCCCTCTGAGTATTAAACTATAGTGGC
  ATTGTCCCTCAAGCTCCCCTCTGCCTTGGCTCCAGAGTCTTCCTCCTCTTCTTCCAGACT
  GGGCACGGTGGCTGCCGTTATTGGCGAAGATAGGCCTCTAGCCCGAGCCGCCCTGACTCC
  TTTACGAGTGGATGATGTCCGCGAAGGTTGACAGCAGGGGCTCGGACTGGTACTCTATGC
  CATGCTTTGCCACACAAGGACTGCCCCAGGGGCACCCACCTTTGGGGCCATTCGCCGTCG
  AGGCCATCGTCGGCACAAAGACGGGCGCCCCAACCCTCCCACCTCGCAGGTGCCCACCCT
  GAACCCGCTCCTTTGAACGCCCGCCCTTGGCGGACCTCCCCCAGTGGGTCTTGGAGCCCG
  GGCCGGAAACCCCAGTTCATGNTCCNNNCCCCCAACAAANGNNCNNNCNAACCNNNNNAC
  NNCNNNNNNNNCNNNNNNNTNNTNCNNNNTNNNNNNNNANCCNNCCTCCNNNNNCNNNNC
  NCNCCCNNCNCCCCCNCCCCCCCCCCCCCCCCCCNNCNNNCNNCNNNNCNNCCNNNNNNN
  NNNNCCCCCCCCCCCTGTCCCNCNG
  
• Restriction Sites: NotI-NotI
• ACCN: BD127299
• Insert Size: 2130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BD127299.1
• RefSeq Size: 2080 bp
• RefSeq ORF: 2080 bp
• Locus ID: 3992
• Cytogenetics: 11q12.2
• Domains: heme_1, FA_desaturase
• Protein Families: Transcription Factors, Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• Gene Summary: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]