ABCA1 (BC034824) Human Untagged Clone

CAT#: SC104214

(untagged)-Homo sapiens, clone IMAGE:4749735

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCA1
• Synonyms: ABC-1; ABC1; CERP; HDLDT1; TGD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC034824, the custom clone sequence may differ by one or more nucleotides
  
  
  GTGAGTGGGGCCGGGACCCGCAGAGCCGAGCCGACCCTTCTCTCCCGGGCTGCGGCAGGGCAGGGCGGGG
  AGCTCCGCGCACCAACAGAGCCGGTTCTCAGGGCGCTTTGCTCCTTGTTTTTTCCCCGGTTCTGTTTTCT
  CCCCTTCTCCGGAAGGCTTGTCAAGGGGTAGGAGAAAGAGACGCAAACACAAAAGTGGAAAACAGTGTCA
  GCTGCTGCTGGAAGTGGCCTGGCCTCTATTTATCTTCCTGATCCTGATCTCTGTTCGGCTGAGCTACCCA
  CCCTATGAACAACATGAATGCCATTTTCCAAATAAAGCCATGCCCTCTGCAGGAACACTTCCTTGGGTTC
  AGGGGATTATCTGTAATGCCAACAACCCCTGTTTCCGTTACCCGACTCCTGGGGAGGCTCCCGGAGTTGT
  TGGAAACTTTAACAAATCCATTGTGGCTCGCCTGTTCTCAGATGCTCGGAGGCTTCTTTTATACAGCCAG
  AAAGACACCAGCATGAAGGACATGCGCAAAGTTCTGAGAACATTACAGCAGATCAAGAAATCCAGCTCAA
  ACTTGAAGCTTCAAGATTTCCTGGTGGACAATGAAACCTTCTCTGGGTTCCTGTATCACAACCTCTCTCT
  CCCAAAGTCTACTGTGGACAAGATGCTGAGGGCTGATGTCATTCTCCACAAGGTAAGCTGATGCCTCCAG
  CTTCCTCAGTAGGGCTGATGGCAATTACGTTGTGCAGCTACTGGAAAGAAATGAATAAACCCTTGTCCTT
  GTAATGGTGGTGAAGGGGAGGGAGGTAGTTTGAATACAACTTCACTTAATTTTACTTCCCTATTCAGGCA
  GGAATTGCCAAACCATCCAGGAGTGGAATATGCAACCTGGCGTCATGGGCCAGCTGGTTAAAATAAAATT
  GATTTCTGGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC034824 unedited
  NNNNGGGTTCAGCAATATGTATCCGACTTACTATNAGGNNCGGCCGCGNNAATTCGGCAC
  CCAGTCAGAAGTGTCTTCAATGGGTGCTGNAGGGNNAAGCCAGCAACAGNAAACAGNNAT
  GATTTCAGGGAGCCCTCCAGGAAAATGCTACAGGAGGAGTGTGCCTGGGTTACTGGAGTA
  GCACAGGAGGAGGGCTTCTAGCTCAGGCTGAGATTTTAGTAAAGGAAATTATGCCACGAT
  GAATCCTGAAGAATGAATAGAAGTGAACCAGATAAAGCACGATAGGAAGCATCTTCCCTT
  ACCTAAGGGAAGACACAGAGGTATATGGAATGGTATGTTAAAAGGTTGGGACTCCAAACA
  GTTCTGTTAAAGCTTAGAGAGTGGTGGGAGAGACTGGAGAAGTTGATTAATTAGTAAATG
  AAGTTGTCTGTGGATTTCCCAGATCCCAGTGGCATTGGATATCCATATTATTTTTAAATT
  TACAGTGTTCTATCTTATTTCCCACTCAGTGTCAGCTGCTGCTGGAAGTGGCCTGGCCTC
  TATTTATCTTCCTGATCCTGATCTCTGTTCGGCTGAGCTACCCACCCTATGAACAACATG
  AATGCCATTTTCCAAATAAAGCCATGCCCTCTGCAGGAACACTTCCTTGGGTTCAGGGGA
  TTATCTGTAATGCCAACAACCCCTGTTTCCGTTACCCGACTCCTGNGGAGGCTCCCGGAG
  TTGTTGGAAACTTTAACAAATCCATTGTGGCTCGCCTGTTCTCAGATGCTNCGAGGCTTC
  TTTTATACAGCCAGAAAGACACCNAGCATGAAGACATGCGCAAAGTTCTGAGAACATTAC
  AGCAGATCAAGAAATCCAGCTCANACTTGAAGCTTNCAGATTCCTGGTGGACAATGAACC
  TCTCTGGGNTCCTATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC034824 unedited
  GGGGGTAAACTGAGTACCGTGGCCCTCACTCTGANGATCGGTTTTTTTCTTTTTTAAAAT
  GGAAGAACCTGTTTTCAAAGAGCTCTTGCTTAGTATTATAATAGTAAAAACTGAAAAAGC
  AGTCTTCTCTGATTGAAACAAGCACAAAAAACTTTTGGCTTAAACTCAGCCACACCCAGC
  TTATTCTTCCCATTCACCCTTTGGGAGATGGCCCAAAATCTGAAAGAACACTAAGAACAC
  TTAGAATACCACTGAACTAAATTATATCACAAACTCCCAGCCAGCCGTACTTTTCTACAA
  AACAAAGTCATGCTGTCCAAGGAAAAGCCTCACATTCCGAAAGCATTAGTGCTTGAAGTT
  TCTCCAGTGAGCAAGTCTACTCACCAGGATTGGCTTCAGGATGTCCATGTTGGAACGAAG
  TACTCGCTCTGCTGCAGCCAGTTTCTCCTTTGGTAGGCCACAAAGCTCAGAAACTTCTTG
  GTCACCAAGTTGAATCATCTCTTCTGATTTTGATCCATTGCACAGACTTGTCAAATGTAA
  CTGGTAGCCTTGCAAAAATACCTTGTGGAGAATGACATCAGCCCTCAGCATCTTGTCCAC
  AGTAGACTTTGGGAGAGAGAGGTTGTGATATAGGAACCCAGAGAAGGTTTCATTGTCCAC
  CAGGAAATCTTGAAGCTTCAAGTTTGAGCTGGATTTCTTGATCTGCTGTAATGTTCTCAG
  AACTTTGCGCATGTCCTTCATGCTGGTGTCTTTCTGGCTGTATAAAAGAAGCCTCCGAGC
  ATCTGAGAACAGGCGAGCCACAATGGATTTGTTAAAATTTCCAACAACTCCGGGAGCCTC
  CCCAGGAGTA
  
• Restriction Sites: NotI-NotI
• ACCN: BC034824
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034824.1
• RefSeq Size: 1004 bp
• RefSeq ORF: 1004 bp
• Locus ID: 19
• Cytogenetics: 9q31.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency. [provided by RefSeq, Sep 2019]