Thymidine Phosphorylase (TYMP) (AK057214) Human Untagged Clone

CAT#: SC104212

(untagged)-Human cDNA FLJ32652 fis, clone SYNOV2001648, moderately similar to THYMIDINE PHOSPHORYLASE PRECURSOR (EC 2.4.2.4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thymidine Phosphorylase
• Synonyms: ECGF; ECGF1; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; TP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK057214, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCC
  AGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGG
  CCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAG
  ATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGAGAGACTTTGGGTCCC
  TGGGGGTGCGACGGTGCCCCACTACCAGCACCGGCCCCAGGGTGCCCCACCGCTGTGGGCTGCCACCCTC
  ACGCGTACCCCCACATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGAC
  CTCGGTGCTGACCCAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAG
  CTTGTGGACAAGCATTCCACAGGGGGTGTGGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK057214 unedited
  TATTTTGGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACCAGGCGAACCCTGAAC
  CCTACGGTCCCGACCCGCGGGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGG
  CGAGGGCAGCGCCCTAAGCAGGTACGGGCGGGGCTCAAGTCGCGAGGCGGGGAAGCGGGA
  GGCAGACACGGACGAGGGCGACACAGACACGGGACCGAGGGGCGGACACCGGAGAGACAC
  GGGAAAGGGGTCGGGACAGGAGCACGTGGCTCAGACACCGACGCCGGGAGGCCGCAGACC
  CCGGACGTGTCAGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAA
  CCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACC
  CTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGGCCGCC
  TGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCG
  CACAGATCGGTGCGTGGGGAGGGTTGGGCGTTCCTGACCCCGACTGGGAGGTCAGCCCGA
  GAGACTTTGGGTCCCTGGGGGTGCGACNGTGCCCCACTACCAGCACCCGGTCCAAGGTGC
  CCCCACCGCTGTGGGCTGTCACCCTCACGCGTACCCCCACATACCAGGGGCCATGCTGAT
  GGCCATCCCAACTCCGGGCATTGGATCTGGAAGAGACCTCGGGTGCTGACCCAGGCCCTG
  GTTTAATCCGGACAACAACCTGAAGTGCCCACAGGCCTTGTCGCCAACAACTTTGTGGAC
  CAGCATTCCCAAGGGGGTGTGGGTGGAAAAGGCAAACTTGTTCTTTCNACCTGCCCTGAG
  GGCATTTGGCTTCGAAGGTAAAAAAACACCTTCCTTTCCAAACGGGGACTTATTCGCCAA
  TGTGAAAACCAATGCATTTCAAAGCGCGTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK057214 unedited
  GAGTTTAAATCCTTTTTTTTTTTGTAGGAGAATCAATTTTCACTATATTACCCAGGCTGA
  TCTCCAACTGGGCTCCTCTGCCTCCCAGAGTGCTGGGACAGGGTCTCACTCTGTTGCCCA
  AGCTGGAGTGCAGTGGCATCGTCCCGGCTCACTGCAGCCTAAACCTCCCAGGCTCAAGTG
  ATCCTCCTGCCTCAGCCTCCTGAGTAGCTGGAACCACGTGCGTGCACCACCACACGCAGC
  GAACTATTTTGTAGAGATGGGGTTTTGCCTTGTTATCCAGGCTGGTTTTGAACTCCTGGG
  TTCAAGGGATCTGCCCTCTTCGGCCTCCCCAAGTGCTAGGACTACAGGTGTGAGCCACCG
  TGCCCCGCCAGCCATCTGTGATTTTGACCAACTTCCCATTTTATGATCCTAATCAAAGTT
  CCACTGATGATTTCGGCCCAAGCCCAGTGACTCATGAGTAACCTTGACCAGTGTTCTCAT
  TAGTGACCCTAATGATCCACCAGTGATCTTTTAGTGATTCTGGCCAGGGTCTCCATCATC
  AACCTGGATCAATGACTGATCCGTGGCGCCCCGTACCTGCTCTGGGCTCTGGATGACATT
  GAATCCAGGAATAGACTCCAGCTTATCCAAAGTGCCTCCTGTGTGCCCCAGACCACGTCC
  GCTGATCATTGGCACCTGGTGGTCAAGGATGCTGAGTACCCTGCACAGTACCCCTCCCCC
  ACCTCTGTGGCTCCCTTCACCTTCCCGGGCTAGGAGTGCANCTGGGATTTGGAAGTATTT
  TAACCANCTTTAGGGGTGCCCAACTGGTGAGGGATGGAAAAGAAGTTTCCCCGATTGAAA
  TCGTCATTCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: AK057214
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK057214.1, BAG51886.1
• RefSeq Size: 2049 bp
• RefSeq ORF: 2049 bp
• Locus ID: 1890
• Cytogenetics: 22q13.33
• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
• Gene Summary: This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]