Glutamine Synthetase (GLUL) (AK092874) Human Untagged Clone

CAT#: SC103453

(untagged)-Human cDNA FLJ35555 fis, clone SPLEN2004773, highly similar to GLUTAMINE SYNTHETASE (EC 6.3.1.2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glutamine Synthetase
• Synonyms: GLNS; GS; PIG43; PIG59
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for AK092874 edited
  GAATTCGGCACGAGGGTTTTGTAAATTTGTGTATTGGTTTTGATTTGTTTCCTCTGATAG
  TTTAGTATTTGGATAGTTTAGTGTTAACCTCAGCTACACTGAAGGAATAGACCTTAGTCC
  TCACAAGTATAAGTTCTAGCTTGGAAGCCTGGGTTCTGCAGTAGCTGTGGAACTTAAGCC
  TCTGAGCTCAGGGATGCAGAGGCATTGAGTTACTACCAAGGGCCTGATCTTTTCTTTAGC
  AGGCATCTGTGTTAATTGTTTCAAAAGGTGGTGATCAGTTTTACAGCCTATTATAAAGGA
  GATTTTTGCCTACTATAAAACTAATCCCCCTGAAAGAGTGAGTAAACATAACTTTTTGTG
  TGTTGACTTCCACAAGGGAAGGAGTTGGCACTTACACTCTGACTTTTGATTCAGTCGTCC
  TTCTTGAGCCATTTTTGCAGGGGATCAGTTTGGAGTGGGCGTTAACAATGTTATTCTTTT
  TTTCTTCTCCAGAACACCTTCCACCATGACCACCTCAGCAAGTTCCCACTTAAATAAAGG
  CATCAAGCAGGTGTACATGTCCCTGCCTCAGGGTGAGAAAGTCCAGGCCATGTATATCTG
  GATCGATGGTACTGGAGAAGGACTGCGCTGCAAGACCCGGACCCTGGACAGTGAGCCCAA
  GTGTGTGGAAGAGTTGCCTGAGTGGAATTTCGATGGCTCCAGTACTTTACAGTCTGAGGG
  TTCCAACAGTGACATGTATCTCGTGCCTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGA
  CCCTAACAAGCTGGTGTTATGTGAAGTTTTCAAGTACAATCGAAGGCCTGCAGGTGTGTT
  ATAGCACAGCTATGGATACCCCTCCTCAATCTGTGAATGCTGTGAAGGGGAGGGAGAAGA
  CATTCTGAAATCAGCATTGGGAAGACTAGGCAATTTCAGCACTATTTTAAGAATCTGAGT
  GATTCTTTTCCCTGAACTTCTGCTTTGAGGAAGAGATAATATGGCCCATCTTTCTATGGT
  CTTCTCTGTTGGTTGCATAAAATAGCATTGGATTTGTCCAGATCTGTTTGCCGGTCTTGG
  AGTCCCCAGTAACAGCCTTCCTGCCTGGAATGTAGGCCAGGACAAATGTAAACCAATGGA
  CAAATGTTTCTCAAAAATTATAGAATGGCTCCAAGTGCCTGAGAAATGAAGAATAAATCT
  GACAACCAGAAGCAGCTGTCTTGTGAATAGAGGGTTAAGTGCCTGGCATTTGGTGCTTGG
  GAGGTGGCCAGAATGCAGATAAGGTGAAAGTTGCCCTGTTCTAAATCCACTCCCATGTGA
  CTTGGTTGTAACTGAGTTTAGTTAAAACTGAAGTCTTTCAGAGTCTTCCTACAGATGTAC
  AATTAACAGCTTCTCTCATTTTTCTGACTCGGTGATCCCAAGAAGGCCTATACTGGGTCA
  GTTCATACCATAGTGCACACCTCAGTTGTATAGAATCCAAGGACTATTCTCCCATCAGCA
  TCGGTATTCAGCATCTATGTCTTTAGATCCCTGATGGCGTATTATTGACTCTTTTTTCTA
  GAGACCAATTTGAGGCACACCTGTAAACGGATAATGGACATGGTGAGCAACCAGCACCCC
  TGGTTTGGCATGGAGCAGGAGTATACCCTCATGGGGACAGATGGGCACCCCTTTGGTTGG
  CCTTCCAACGGCTTCCCAGGGCCCCAGGGTAAGTCTCCTTGGGTTAGAGGTGAAATTCCC
  AGAAGTGTCTAACTGTGCAGGAATGCCCCTTCCCAGGGATGGGAATGACTTTCAGAATCA
  AGAAGCAAAATAATACAGTAAAGGCGAAACAGCCCTCACATCACCAAAGTCCAAAAATGG
  ATATGAATATATAAAGTAAGGTTTTAGGGGGAACGTTTGGCCCCACTGAAGCTGTGGTGA
  AGAGGAACTCCCCTATTGCCCCTCCCCTGCCCCGCACCTGCAGATGAAGGCAAGGATAGT
  GATTCAAGAGGGCAAGGCTTAAGGGCCTTCTGATCTCTGACTTTGGGATTCTCTGGATTT
  CTTGACTCTTAGCGTTTTGTCCTGATGCTTCTGTAGGTCCATATTACTGTGGTGTGGGAG
  CAGACAGAGCCTATGGCAGGGACATCGTGGAGGCCCATTACCGGGCCTGCTTGTATGCTG
  GAGTCAAGATTGCGGGGACTAATGCCGAGGTCATGCCTGCCCAGGTAAGTATAGCTCCAA
  TCCATCAATGAAGAAGGGTAGGTAGGTGCACATAGGACTTTTGCTAGTAAGGGCTGCTGA
  TACACCACTCACTAACCCAAAACCTAAGAACGGGTTGGAGTACAGGTGAGAAGAGAACAG
  GTTTAGGAGATTCTGAGTTGGAGTGAGCAGTTAGCTTTGTTTTAATGGCCAAGCTTCTCG
  TTTCTAGTGGGAATTTCAGATTGGACCTTGTGAAGGAATCAGCATGGGAGATCATCTCTG
  GGTGGCCCGTTTCATCTTGCATCGTGTGTGTGAAGACTTTGGAGTGATAGCAACCTTTGA
  TCCTAAGCCCATTCCTGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAGCACCAA
  GGCCATGCGGGAGGAGAATGGTCTGAAGTGAGTACCTTCTGCTGGGGCCATCTTTAATCT
  CCTGTGGCAGAAAACTTGGGAGGAGACTTAGCAATCTCTCAGCAAAGTCTCCTTTGCAGG
  ATGACTTGCAAATATTTGCCAAAGATGAGTAAACTTGACTTCTCAGTCTGGACGTACTTT
  AGGTGTTGACACTTGCCTTCACATTCTCTCATTTTGTTCCTATTTGAAAAATACCAAATA
  ATACTTCTGATTCACAGTGATAAATATTTGTTATAATTTATATAATATATATTAGTCATA
  TATCATTATATAAATATATATCGATATATATATTTGTGACATGTCATGGTGACAGGGAAA
  AGTTGACAAATTCATGCATTTGAAAATCTTTTAGAACTAAATTAGTAACAATACAGGCAT
  GTGGATAAGCTTAATGCTTATGAGGGGGAGAAAGTTTCAAATGATTAGTCTTTTCAACAA
  ACAGTAACTTTGTACTGCTTGTCGGGCACTGTTCTCACCACTGAGACACACAGGTAAGAA
  GATGCAGCCACTGCCCTCATGAAGTATTTGTTCTACTGGTATCATATTTTGGTGCACTTC
  ATTCTTGGCTCCATACCTGGAGACAAGGTTGGACTGCCATCTTTTCTGTTTACTCTAGGT
  ACATCGAGGAGGCCATTGAGAAACTAAGCAAGCGGCACCAGTACCACATCCGTGCCTATG
  ATCCCAAGGGAGGCCTGGACAATGCCCGACGTCTAACTGGATTCCATGAAACCTCCAACA
  TCAACGACTTTTCTGCTGGTGTAGCCAATCGTAGCGCCAGCATACGCATTCCCCGGACTG
  TTGGCCAGGAGAAGAAGGGTTACTTTGAAGATCGTCGCCCCTCTGCCAACTGCGACCCCT
  TTTCGGTGACAGAAGCCCTCATCCGCACGTGTCTTCTCAATGAAACCGGCGATGAGCCCT
  TCCAGTACAAAAATTAAGTGGACTAGACCTCCAGCTGTTGAGCCCCTCCTAGTTCTTCAT
  CCCACTCCAACTCTTCCCCCTCTCCCAGTTGTCCCGATTGTAACTCAAAGGGTGGAATAT
  CAAGGTCGTTTTTTTCATTCCATGTGCCCAGTTAATCTTGCTTTCTTTGTTTGGCTGGGA
  TAGAGGGGTCAAGTTATTAATTTCTTCACACCTACCCTCCTTTTTTTTCCCTATCACTGA
  AGCTTTTTAGTGCATTAGTGGGGAGGAGGGTGGGGAGACATAACCACTGCTTCCATTTAA
  TGGGGTGCACCTGTCCAATAGGCGTAGCTATCCGGACAGAGCACGTTTGCAGAAGGGGGA
  CTCTTCTTCCAGGTAGCTGAAAGGGGAAGACCTGACGTACTCTGGTTAGGTTAGGACTTG
  CCCTCGTGGTGGAAACTTTTCTTAAAAAGTTATAACCAACTTTTCTATTAAAAGTGGGAA
  TTAGGAGAGAAGGTAGGGGTTGGGAATCAGAGAGAATGGCTTTGGTCTCTTGCTTGTGGG
  ACTAGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTTAGTATAAACTGATG
  GATATCCCTACCTTGAAAGAAGAAAAGGTTCTTACTGCTTGGTCCTTGATTTATCACACA
  AAGCAGAATAGTATTTTTATATTTAAATGTAAAGACAAAAAACTATATGTATGGTTTTGT
  GGATTATGTGTGTTTTGCTAAAGGAAAAAACCATCCAGGTCACGGGGCACCAAATTTGAG
  ACAAATAGTCGGATTAGAAATAAAGCATCTCATTTTGAGTAGAGAGCAAGGGAAGTGGTT
  CTTAGATGGTGATCTGGGATTAGGCCCTCAAGACCCTTTTGGGTTTCTGCCCTGCCCACC
  CTCTGGAGAAGGTGGGCACTGGATTAGTTAACAGACGACACGTTACTAGCAGTCACTTGA
  TCTCCGTGGCTTTGGTTTAAAAGACACACTTGTCCACATAGGTTTAGAGATAAGAGTTGG
  CTGGTCAACTTGAGCATGTTACTGACAGAGGGGGTATTGGGGTTATTTTCTGGTAGGAAT
  AGCATGTCACTAAAGCAGGCCTTTTGATATTAAATTTTTTAAAAAGCAAAATTATAGAAG
  TTTAGATTTTAATCAAATTTGTAGGGTTTCTAGGTAATTTTTACAGAATTGCTTGTTTGC
  TTCAACTGTCTCCTACCTCTGCTCTTGGAGGAGATGGGGACAGGGCTGGAGTCAAAACAC
  TTGTAATTTTGTATCTTGATGTCTTTGTTAAGACTGCTGAAGAATTATTTTTTTTCTTTT
  ATAATAAGGAATAAACCCCACCTTTATTCCTTCATTTCATCTACCATTTTCTGGTTCTTG
  TGTTGGCTGTGGCAGGCCAGCTGTGGTTTTCTTTTGCCATGACAACTTCTAATTGCCATG
  TACAGTATGTTCAAAGTCAAATAACTCCTCATTGTAAACAAACTGTGTAACTGCCCAAAG
  CAGCACTTATAAATCAGCCTAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK092874 unedited
  NGGGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGGTTTTG
  TAAATTTGTGTATTGGTTTTGATTTGTTTCCTCTGATAGTTTAGTATTTGGATAGTTTAG
  TGTTAACCTCAGCTACACTGAAGGAATAGACCTTAGTCCTCACAAGTATAAGTTCTAGCT
  TGGAAGCCTGGGTTCTGCAGTAGCTGTGGAACTTAAGCCTCTGAGCTCAGGGATGCAGAG
  GCATTGAGTTACTACCAAGGGCCTGATCTTTTCTTTAGCAGGCATCTGTGTTAATTGTTT
  CAAAAGGTGGTGATCAGTTTTACAGCCTATTATAAAGGAGATTTTTGCCTACTATAAAAC
  TAATCCCCCTGAAAGAGTGAGTAAACATAACTTTTTGTGTGTTGACTTCCACAAGGGAAG
  GAGTTGGCACTTACACTCTGACTTTTGATTCAGTCGTCCTTCTTGAGCCATTTTTGCAGG
  GGATCAGTTTGGAGTGGGCGTTAACAATGTTATTCTTTTTTTCTTCTCCAGAACACCTTC
  CACCATGACCACCTCAGCAAGTCCCACTTAAATAAAGGCATCAAGCANGTGTACATGTCC
  CTGCCTCAGGNTGAGAAAGTCCAGGCCATGTATATCTGGATCGATGGTACTGGAGAAGGA
  CTGCGCTGCAAGACCCCGACCTGGACAGTGAGCCCAAGTGTGTGGAAGAAGTGCCTGAGT
  GGAATTTCGATGGCTCCAGTACTTTACAGTCTGAGGGTTCCAACAGTGACATGTATCTCG
  TGCCTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGACCCTAACAAGCTGGGTGTATGTG
  AAGTTTTCAAGTACAATCGAAGGCCTGCNNAGTGTGTATAGCACAGCTATGGATACCCCT
  NCTCATCTGTATGCCTGTGTGAAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: AK092874
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK092874.1, BAG52617.1
• RefSeq Size: 2265 bp
• RefSeq ORF: 2265 bp
• Locus ID: 2752
• Cytogenetics: 1q25.3
• Domains: gln-synt_N
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]