Laminin (LAMA1) (AK091949) Human Untagged Clone

CAT#: SC103296

(untagged)-Human cDNA FLJ34630 fis, clone KIDNE2015546, highly similar to LAMININ ALPHA-1 CHAIN PRECURSOR

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Laminin
• Synonyms: LAMA; PTBHS; S-LAM-alpha
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC103296 sequence for AK091949 edited (data generated by NextGen Sequencing)
  ATGCTGATCGGAGGCAACATTGAGGTACATGTCAATCCTGGGGATGGGACAGGCCTGAGA
  AAAGCTCTCCTGCACGCTCCCACGGGTACCTGCAGTGATGGACAAGCGCATTCCATCTCC
  TTGGTCAGGAATCGGAGAATTATCACTGTCCAATTGGATGAGAACAATCCTGTGGAAATG
  AAGTTGGGCACATTAGTAGAAAGCAGGACGATAAATGTGTCCAATCTGTACGTCGGGGGA
  ATTCCAGAGGGAGAGGGGACGTCACTGCTCACAATGAGAAGATCGTTCCATGGCTGTATC
  AAAAACCTGATCTTCAATTTGGAACTTTTGGATTTCAACAGTGCAGTTGGCCATGAGCAA
  GTCGACCTGGACACCTGCTGGCTGTCAGAAAGGCCTAAGCTGGCTCCCGATGCAGAGGAC
  AGCAAGCTCTTGCCAGAGCCCCGGGCTTTTCCAGAACAGTGTGTGGTGGATGCAGCTCTG
  GAGTACGTTCCCGGCGCTCACCAGTTTGGTCTCACACAAAACAGCCATTTCATCTTGCCT
  TTTAATCAGTCGGCTGTCAGAAAGAAGCTCTCGGTTGAGCTAAGCATCCGCACGTTCGCC
  TCCAGCGGCCTGATTTACTACATGGCTCATCAGAACCAAGCAGACTACGCTGTGCTCCAG
  CTGCACGGGGGCCGCCTCCACTTCATGTTTGACCTTGGCAAAGGCAGAACAAAGGTCTCT
  CACCCTGCACTGCTCAGTGATGGCAAGTGGCACACGGTCAAGACAGACTATGTTAAAAGA
  AAAGGCTTCATAACTGTCGACGGCCGAGAGTCTCCCATGGTGACTGTGGTGGGAGATGGA
  ACCATGCTGGATGTGGAGGGTTTGTTCTACCTAGGAGGCCTGCCCTCCCAGTACCAGGCC
  AGGAAAATTGGAAATATCACCCACAGCATCCCTGCCTGCATTGGGGATGTGACGGTTAAC
  AGCAAACAGCTGGACAAGGACAGCCCGGTGTCTGCCTTCACGGTGAACAGGTGCTACGCA
  GTGGCCCAGGAAGGAACATACTTTGACGGAAGCGGATATGCAGCTCTTGTCAAAGAGGGC
  TACAAAGTCCAGTCAGATGTGAACATCACACTGGAGTTTCGAACCTCCTCGCAGAATGGC
  GTCCTCCTGGGGATCAGCACTGCCAAAGTGGATGCCATTGGACTAGAGCTTGTGGACGGC
  AAGGTCTTGTTCCATGTCAACAATGGTGCTGGCAGGATAACAGCTGCATATGAGCCCAAA
  ACCGCCACTGTGCTCTGTGATGGAAAATGGCACACTCTTCAAGCTAACAAAAGCAAACAC
  CGTATCACTCTGATTGTTGACGGGAACGCAGTTGGCGCTGAAAGTCCACACACCCAGTCT
  ACCTCAGTGGACACCAACAATCCCATTTATGTTGGTGGCTATCCTGCTGGTGTGAAGCAA
  AAATGCCTGCGCAGCCAGACCTCGTTCCGCGGGTGTTTGAGGAAGCTAGCTCTGATTAAG
  AGCCCGCAGGTGCAGTCCTTTGACTTCAGCAGAGCGTTCGAACTGCACGGAGTTTTCCTT
  CATTCCTGTCCTGGGACNGAGTCCTGA
  
  Clone variation with respect to AK091949.1
  1450 t=>c;1578 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK091949 unedited
  CCATATGGATCCGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGCTACATTG
  AATTGCCACCCAAATCTTTGTCACCAGAATCAGAATGGCTGGTAACATTTGCCACCACGA
  ACAGCAGTGGCATCATCCTGGCTGCCCTCGGCGGGGATGTGGAGAAGCGGGGTGACCGTG
  AGGAAGCACACGTGCCCTTCTTTTCCGTCATGCTGATCGGAGGCAACATTGAGGTACATG
  TCAATCCTGGGGATGGGACAGGCCTGAGAAAAGCTCTCCTGCACGCTCCCACGGGTACCT
  GCAGTGATGGACAAGCGCATTCCATCTCCTTGGTCAGGAATCGGAGAATTATCACTGTCC
  AATTGGATGAGAACAATCCTGTGGAAATGAAGTTGGGCACATTAGTAGAAAGCAGGACGA
  TAAATGTGTCCAATCTGTACGTCGGGGGAATTCCAGAGGGAGAGGGGACGTCACTGCTCA
  CAATGAGAAGATCGTTCCATGGCTGTATCAAAAACCTGATCTTCAATTTGGAACTTTTGG
  ATTTCAACAGTGCAGTTGGCCATGAGCAAGTCGACCTGGACACCTGCTGGCTGTCAGAAA
  GGCCTAAGCTGGCTCCCGATGCAGAGGACAGCAAGCTCTTGCCAGAGCCCCGGGCTTTTC
  CAGAACAGTGTGTGGTGGATGCAGCTCTGGAGTACGTTCCCGGCGCTCACCAGTTTGGTC
  TCACACAAAACAGCCATTTCATCTTGCCCTTTAATCAGTCGGCTGTCAGAAAAGAGCTCT
  CGGTTGAGCTAAGCATCCGCACGTTCGCCTCCAGCGGNCCTGATTACTACATGGCTCATC
  AGAACCAAGCAGACTACGCTGTGCTCCAGCTGCACGGGGGCCGCCTNCACTTCATGTTTG
  ACCTTNGGCAAGCAGAACAAGGTCTCTCACCTGCACTGCTCANTGATGGCCAGTGCACAC
  GTCAAGACNGACTATGTAAAGAAAAGCTNCATACTGTCGACGCCGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK091949 unedited
  NNGGGTTTGCTCCTGTTTTGGTACTTTTTTAAAAGCTCAAATGNAAAAACATAAAATACT
  ATCAAGTAATCAACAGAACATTCAATGTGTATAAAGATTTTTTTAAAAATACGTTTAAAA
  AGAGAGCCAGGGAATTCAATTTACATTTTAGACCATTTAATGGAGGTATTTGTTGCACAT
  GTGGTTTTAGTGTAACGTAAACACAACATCTCTCCCCAGAAACACTTAACCTGAGTTGGA
  AATGAAATATGAACTGAAGAGATTCTTAATTCACACATACACTTCTCCTCAAAATATTAG
  CAATGATTCCAACTGAGGATTCTGCTTGAAGTTCAGGACTCGGTCCCAGGACAGGAATGA
  AGGAAAACTCCGTGCAGTTCGAACGCTCTGCTGAAGTCAAAGGACTGCACCTGCGGGCTC
  TTAATCAGAGCTAGCTTCCTCAAACACCCGCGGAACGAGGTCTGGCTGCGCAGGCATTTT
  TGCTTCACACCAGCAGGATAGCCACCAACATAAATGGGATTGTTGGTGTCCACTGAGGTA
  GACTGGGTGTGTGGACTTTCAGCGCCAACTGCGTTCCCGTCAACAATCAGAGTGATACGG
  TGTTTGCTTTTGTTAGCTTGAAGAGTGTGCCATTTTCCATCACAGAGCACAGTGGCGGTT
  TTGGGCTCATATGCAGCTGTTATCCTGCCAGCACCATTGTTGACATGGAACAAGACCTTG
  CCGTCCACAAGCTCTAGTCCAATGGCATCCACTTTGGCAGTGCTGATCCCCAGGAGGACG
  CCATTCTGCGAGGAGGTTCGAAACTCCAGTGTGATGTTCACATCTGACTGGACTTGTAGC
  CCTCTTTTGACAGAGCTGCCTATCCGCTTCCGTCAAAGTATGGTTCCTTCCTGGGCCACT
  GCGTTACAC
  
• Restriction Sites: NotI-NotI
• ACCN: AK091949
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK091949.1, BAG52448.1
• RefSeq Size: 2825 bp
• RefSeq ORF: 1587 bp
• Locus ID: 284217
• Cytogenetics: 18p11.31
• Domains: LamG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]