CBL (AK092300) Human Untagged Clone

CAT#: SC102986

(untagged)-Human cDNA FLJ34981 fis, clone OCBBF2000982

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CBL
• Synonyms: C-CBL; CBL2; FRA11B; NSLL; RNF55
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK092300, the custom clone sequence may differ by one or more nucleotides
  
  
  TCAGGACCCAAAGATCCTTTAGTCAGTTGTTGGGTCTTCCAAGAGCCAGACATTAATACAGATTGAACTC
  CATCAGTCCCCTAATTGTCAGCCTTTACCTCCCTCCCAGAGCAAGGAGTTTAGGGATTCTAAAGCTTAGT
  GTCCACACATCATTCTACCAGACCTTAGAGCTTTAGAAGCTCAATCTAAAATACTGTAACTCAGCATAAA
  CTATTACTATCACTCCTTTGAACTCAGTCTCCATGAGCAGTGTTTTGTTGGAAATACATAGAACGGCTTA
  ATGCCTAGAGGGTGGTGGATAGTGAAGGACGGTCAAGGTTATATTTTTGACTGCTTAGGGATTCTTTGGA
  TACAAGAAACAGAAATGTTCAAGCGGAATAAAGGAGGGAGTGGAGTTGTGGTAAGGATGCAGGGTATTTC
  GCAGAACCCAGGACGGGAAGTGCCTTTGGTTCTTGGGTGGAGCTGGAACTGCAGAGCTTTGCACCTAGTC
  CTTTCTCCCGCTTCACAGTCTGCTTATGGTATATGTGGCCCCCAAATAGGCACTCTAGTCCTCAAGTCTA
  CACCACCTTCCAACTCTGGGGATCACCATGAACAAATTCTCAATTTCCCATACTTAATTTTTTTTTTTTT
  TGAGATGGAGTCTCGCTGTGTCGCCCAGGCTGGAGTGCAGTGGTGCAGTCTCAACTCACCACAACCTCCG
  CCTCCCAGGTTCAAGCAGTTCTCTGCCTCAACCTCCCGAGTAGCTGGGATTACAGGCGCCTGCCACCATG
  CCCAGCTAATGTTCATATTTTTAGTAGAGACAGGGTTTCACCGTCTTGGCTAGGCTGGTCTTGAACTCCT
  GACCCTCATGATCCACCCACCTCGGCCTCCCAAAGTGCTAAGATTACAGGCGTGAGCCACCGCGCCCGGC
  CCATACTTCGTATTCTTAAAAAAAACTACACTCAGCCCAGCACATTGATCAAGTATCTATCTCTGAGCAG
  TTGGCCTTGCCAGGGAGAGCAGAGATGTGGCAGGCTCCTTCAGCTGGAGACAGGGAGCTTCTCAGAGAAG
  TGAGCAGAGACTCCACAGACACCCTAAAAAGGCTTCTACTCAAGAAGTAAAGCCACTACTCCTGCCTTTT
  TGCTTAGTGGACAGGAAGGCACAGGAGTTTGTCTGGGACATCATAGAAATTCTTAGGTTTAACTTAATTC
  TGGTCATTGTCTTCTTTATTTCCTGTTTTTCTTCCCTTTGTCAGTCTTCGCATCCAAGATTTCTTCCCTC
  CCTCTTGTGGGCCAGCCTGTCCTGTTCCAGAGCTAGCCTGTTCCTGGGTAGCCTTCCTTAGCCTCCATTC
  AGCCTCAGGTCTTTTGCCTTCTTCCGTGTTTATTTAGAGAGCAGAATCTAATAACGGGTTCCACTGTAGC
  CACTATCCATGGACTTCTGGGTCCTCTTCAGGTTTGAGTGCTTGAAAATGTTCATTCTCTGGGCTTGTGG
  CCTGTCTCCTCCACTCTCCTCCTCACCCTCTCGCTCCTTCCTGTGTGAGGGCCGCTCTGCAGTAATGTTC
  TCAGGCAAGCCTTCCTAGGCACCTCAGAAACTACTTTGCCAGAGCCAGTAAGAATATATAATATTGGAGC
  AGTTGCCAGGATAGAAATTAAATATAGATTCCAGTTTAGGATAGAGTTTTTACCGAGAGCTCTTTAGACA
  GTATACCTGTGTCTTCTCTGGCAATTGCTTTCATTTTAGTCCTATATAAAAGCTTTCCTTTTCTGTTTTT
  TTTTAAAACTATGCTTTTGCTTGCCTAAATCTTTTGATCTTATATTTCTCTCATCTCAGAGCCTGTCCTG
  AGTTGTAAGGTATTTCATACTGCCTTACTTAAAAGTTTTTTAAACTACTAGAGTCATTTGATACACACAG
  AAGTTACCTAATAATCCAAAGATGTCCATCAAGGGAGGAAGGGTGGGTCATCAGACTTTGCCTTTGATGT
  TGTAGACTAGGCTCCTGAGTTAAGCAGCAGAGGGACAGCAGTGCCATGTGCCTTCACTGTGTCCCAGGAA
  ATCTGGGTTGGTTCCAGTGGGAAATACCAGTATTTCTTGGTTCTGGAAAGTAGCAAAAGAGTAGGAGATG
  GGGAAATAGGGATGGGGAGAGCAAGCCCCGCATGTCCATGGCGAGTCAGGTGGGGAGCACGGGTGGAAGG
  GCCGGCTGTTGACAGACAGACTAAGCTGTGTGGTGCTCTTGCCGCCCCTTCCTGGGTACAGAGCTTGAGA
  AAAATGCAGCCGACCACTCCCTGTGTTTGTACAGAGCAAAGCCCAAAAGCCAACCTCAGATCTCCTGATT
  TGGCAGCTGAAGAAATCAGCAGAGTCCTGATTGCCTGATTCAGTCCCAAAAATGAATGTCAGGCCCCGCC
  CCCTCCCCACCAACATTGCCTCTCCTACATTCTCCTTCTGCCCCTAAATCAGACAGGAGGCCAGAGAGGA
  GTATTGCTCAATGCGTGCTATGTGCAACTCCTCAGGCCTTGTGCCACCTCCATGCTGAGCCCTGAAGCAG
  GGTGTCCTGGGTGCCTGTGTGTCAGCTCCCTCCTCTCTACCTACCTCTGACCTTCTTGTGGGTGAGGGTG
  GCCATGCTTATGGCCATCTTAAAACTGGAGAGGCAGAGAACTACTTATGAGTCTGTAGACCACGTGTTGT
  CTTCCATGGCCTGTTTCTCCTGCTGTCTGGGTGAGTGAGCCTGCAACGCAATGCCCATGAGAGTAAATGC
  CTCCTGACCTACCCTGCTCAGCACTGTTCTAGTGTCTTGGCCTTGAAAGAAAAGCCTGACTTCCTGCTGA
  CACATGTGGTAGGGGCATGGCAGCTATGAGGCACCTCCTACGTCTGTTTTCTGGCTGTGGTGACTTGGGA
  TTTTTAACCTTATATATCTTTTTCCTTTACTCAAAACAAAACAATTTTTAGCACACTG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK092300 unedited
  TCAGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAAATTCGCACGAGGCTTTGATA
  ATGCCTGGGAGATTCCTTGGTGTAAGTGTCATGGATACCGACTGTTTTTATGTTGGAATT
  TGTTCCAACATAATTAGAATCTGTTTGGTGAGTTGAAAGGTAAGTTGGCTCAGAGTTGCA
  CAGTAGGGCATTAAATGTTTAAGCAAAGCATCTGCCCACACTCCCCTTTCCAATCTAGTG
  CCTTCCTTGAACTTTTTCCTGAGCTGCTACGTCCCTAATCCCCCTTGTTGGGAGGATTTT
  CGTATCACCCTTATGGGACCTGTCACCATGTCCTGTACTATTTGGAATTGGTTTTCCAGT
  CTTTCAACAACCGTTGTGGCTAACTATGTTTTAGAAGGGCTGGAGGTGTGGGCCCTGTCT
  TCGGGTCTCAGGACCCAAAGATCCTTTAGTCAGTTGTTGGGTCTTCCAAGAGCCAGACAT
  TAATACAGATTGAACTCCATCAGTCCCCTAATTGTCAGCCTTTACCTCCCTCCCAGAGCA
  AGGAGTTTAGGGATTCTAAAGCTTAGTGTCCACACATCATTCTACCAGACCTTAGAGCTT
  TAGAAGCTCAATCTAAAATACTGTAACTCAGCATAAACTATTACTATCACTCCTTTGAAC
  TCAGTCTCCATGAGCAGTGTTTTGTTGGAAATACATAGAACGGCTTAATGCCTANAGGGT
  GGTGGATAGTGAAGGACGGTCAAGGTTATATTTTTGACTGCTTANGGATTCTTTGGATAC
  AAGAACAGAAATGTTCAAGCGGAATAAAGNAGGAGTGGAGTTGTGGTAGGATGCAGGGTA
  TTTCGCAGACCCAGACGGGAAGTGCCTTTGTCTTGGTGGAGCTGAACTGCAAGCTTGCAC
  TAGTCTTTCTCCGCTACAGCTGCTATGGATATGTGCCCCAAAGCACCTATNCTCAGTTCA
  CACTTCATTTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK092300 unedited
  GCTCATTTATGNACCGCGGCACGCAATTCTNANGATCGAGTTTTTTTTTTTTTTTTTTCA
  GTGTGCTAAAAATTGTTTTGTTTTGAGTAAAGGGAGAAAAGATATATAAGGTTAAAAATC
  CCAAGTCACCACAGCCAGAAAACAGACGTAGGAGGTGCCTCATAGCTGCCATGCCCCTAC
  CACATGTGTCAGCAGGAAGTCAGGCTTTTCTTTCAAGGCCAAGACACTAGAACAGTGCTG
  AGCAGGGTAGGTCAGGAGGCATTTACTCTCATGGGCATTGCGTTGCAGGCTCACTCACCC
  AGACAGCAGGAGAAACAGGCCATGGAAGACAACACGTGGTCTACAGACTCATAAGTAGTT
  CTCTGCCTCTCCAGTTTTAAGATGGCCATAAGCATGGCCACCCTCACCCACAAGAAGGTC
  AGAGGTAGGTAGAGAGGAGGGAGCTGACACACAGGCACCCAGGACACCCTGCTTCAGGGC
  TCAGCATGGAGGTGGCACAAGGCCTGAGGAGTTGCACATAGCACGCATTGAGCAATACTC
  CTCTCTGGCCTCCTGTCTGATTTAGGGGCAGAAGGAGAATGTANGATAGGCAATGTTGGT
  GGGGAGGGGGGCGGGGCCTGACATTCATTTTTGGGACTGAATCANGCAATCAGGACTCTG
  CTGATTTCTTCAGCTGCCAAATCAGGAGATCTGAGGTTGGCTTTTGGGCTTTGCTCTGTA
  CAAACACAGGGAGTGGTCGGCTGCATTTTTCTCAAGCTCTGTACCCAGGAAGGGGGCGGG
  CAGAGCACCACACAGCTTAATCTGTCTGTCAACAGCCGGGCCTGCCACCCGTGCTCCCCA
  CTGACTCGCATGGACATGCGGGGCTTGCTCTCCCATCCTATTTCCCATCTNCTACTCTTT
  GCTACTTTCA
  
• Restriction Sites: NotI-NotI
• ACCN: AK092300
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK092300.1
• RefSeq Size: 2928 bp
• RefSeq ORF: 2928 bp
• Locus ID: 867
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
• Gene Summary: This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]