RANTES (CCL5) (AK092450) Human Untagged Clone

CAT#: SC102926

(untagged)-Human cDNA FLJ35131 fis, clone PLACE6008824

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RANTES
• Synonyms: BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP; UG0882E07
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK092450, the custom clone sequence may differ by one or more nucleotides
  
  
  AAGTACTTTATAGATTCAGAAGTAAATGCTGTCCTGTTCTCCATCAGCTGTTCGTTTGTTACAGATTTTG
  TATTTCTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACACAGGCTGGAGTACAGTGGTGCGGTCT
  CGGCTCCCTGCAACCTCTGCCTCCCGGGTTCAAGCGATTCTTCTGCCTCAGCCTCCTGAGTAGCTGGGAC
  TACAGGCGCGTGCCACCACCCCTGGCTAATTTTTGTATTTTTTTTTCTTTTTTTTTTGAGAAGGAGTCTC
  TCTCTGTCACCCAGGCTGGAGTGCAGTGGCACGATCTCGGCTCACTGCAAGCTGCGCCTCCTGGGTTCAC
  GCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGAATTACAGGCGTCCGCCACCGTGCCCGGCTAATTTT
  TTTGTATTTTTAGTAGAGATGGGGTTTTGCCATATTGGCCAGGCTGGTCTCGAACTCCTGACCTCAAGTG
  ATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCACCTGGCCAGATCTTTG
  TATGTCTTAAGTGTTTCAAAGTTATAAGCATTTTTCTGGGGGGATGTCCATTTTGGAGGGATCCATTTTG
  ATCCTTTGTACTCTATAATGTGAACTTTCCCCTGTTCCAACACTTAAAAGAGAATTATTAGCACATAATC
  TAAAAGATGGAATTTTTTTTTTTTCTTGAGACAGAGTCTCGCTCTGTCGCTAGGCTGGAGTGCAGTGGCG
  CGATCTTGGCTCACTGCAACCTCTGCCTCCTGGGTTTAAGCGATTCTCCTGCCTCAGCCTCTGGAGTAGC
  TGGGACTACTCGTGCATGCCACCACGCCCGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCAT
  GTTGGCCAGGATGGTCTCGATCTCTTGACCTCGTGATCCACCTGCCTCGGCCTCCCAAATTGCTGGGATT
  ACAGCACTGTGCCCTCCTAGGAAATTATTTTTTAAGTGAAATTTTATTTTTATTTTTTTTAGGATTTTGG
  TAGAGAATGAGTAGGCCTACTCATCAATATCAAACAGGACATTTAGTTTCTTTCCTTAGAACAGACATAA
  ATTTAATTTCATGGTAATATGATAATAAGAAAATGCTTCTATTTTTCTTTAGCACCTCCATGGTTCTCAT
  ATACCCATGTCTGTAAAAAGTGACATGAGAATTTTGTTGGGTTACATTTTATTGTATTTATTAGATTCGC
  TTATATAGATGACTTAGGCAGAAATAAAGTCATGTCTTTAGAAGGTGAACAAGCCAACTTGTGATGGCCT
  GCCTTTTGCTTTTGGCAGTTGGGATGAGAACAATTGACTCTCCCATTGGTTGTTAGATAGTTGAAATGGT
  GCGTTGGTGGTCATACTTAGTGTTCTAGGCTGTGAAATCATGGAGTTCTTCCACTTCCAAGAATGACTCA
  TTTGCTGTTGGATTCTAGTACAGAATTTAGCAGCCTGATGTGTCCCCAAACTGATTTAATTTCTACTGAA
  GTGCCCTTGTGTACATTTGTTTTGTAATTTACCAAAGTACTACCTGAGTGTATAATGACTCCTGCAGTGA
  GTTAATGTAATTGCTGCTTTGACCATTGTTTTAAATCTGTGTACTAGAGTAACTGTGAGCAGAATGAAAT
  CACATTATCTCAGTGTTCAAAATATCATTCTAATAAAGTACATGCATTAAACAATTTT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK092450 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTATAGGCATAG
  TGTAAATTACATTAATGTAGTAAAACATCAATTGTGGTTCGGTTTGTCTTTCATTTATGT
  GTAGTATAGAAATCACCTTTCTAATATGTGTTGCCAAACTATTTGCCACCATCTATTTGG
  TGAAATATTCATTGTCATTGTGATTTTCCACAAGTATAAGTTCTTAAGTACTTTATAGAT
  TCAGAAGTAAATGCTGTCCTGTTCTCCATCAGCTGTTCGTTTGTTACAGATTTTGTATTT
  CTTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACACAGGCTGGAGTACAGTGGT
  GCGGTCTCGGCTCCCTGCAACCTCTGCCTCCCGGGTTCAAGCGATTCTTCTGCCTCGGCC
  TCCTGAGTAGCTGGGACTGCAGGCGCGTGCCACCACCCCTGGCTAATTTTTGGATTTTTT
  TTTCTTTTTTTTTTTGAGAAGGAGTCTCTCTCTGTCACCCAGGCTGGAGTGCAGTGGCAC
  GATCTCGGCTCACTGCAAGCTGCGCCTCCTGGGTTCACGCCATTCTCCTGCCTCAGCCTC
  CCGAGTAGCTGGAATTACAGGCGTCCGCCACCGTGCCCGGCTAATTTTTTTGTATTTTTA
  GTAGAGATGGGGTTTTGCCATATTGGCCAGGCTGGTCTCGAACTCCTGACCTCAAGTGAT
  CCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCACCTGGCCA
  GATCTTTGTATGTCTTAAGTGNTTCAAAGTTATAAGCATNTTTTCTGGGGGGGATGTCCA
  TTNNNTGGAGGGATCCCATTNTGATCCCTTTGNNACTCTATATGNGAAACTTTNCCCTGT
  TNNCACACTCANNAGAGAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK092450 unedited
  TAAATCTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTTTA
  ATGCATGTACTTTATTAGAATGATATTTTGAACACTGGAGAAATGTGATTTCATTCTGCT
  CACAGTTACTCTAGTACACAGATTTAAAACAATGGTCAAAGCAGCAATTACATTAACTCA
  CTGCAGGAGTCATTATACACTCAGGTAGTACTTTGGTAAATTACAAAACAAATGTACACA
  AGGGCACTTCAGTAGAAATTAAATCAGTTTGGGGACACATCAGGCTGCTAAATTCTGTAC
  TAGAATCCAACAGCAAATGAGTCATTCTTGGAAGTGGAAGAACTCCATGATTTCACAGCC
  TAGAACACTAAGTATGACCACCAACGCACCATTTCAACTATCTAACAACCAATGGGAGAG
  TCAATTGTTCTCATCCCAACTGCCAAAAGCAAAAGGCAGGCCATCACAAGTTGGCTTGTT
  CACCTTCTAAAGACATGACTTTATTTCTGCCTAAGTCATCTATATAAGCGAATCTAATAA
  ATACAATAAAATGTAACCCAACAAAATTCTCATGTCACTTTTTACAGACATGGGTATATG
  AGAACCATGGAGGTGCTAAAGAAAAAAGAAGCATTTTCTTATTATCATATTACCATGAAA
  TTAAATTTATGTCTGGTCTAAGGAAAGAAACTAAATGTCCTGTTTGATATTGATGAGTAA
  GCCTACTCATTCTCTACCAAAATCTAAAAAAAATAAAAATAAAATTTCACTTTAAAAAAT
  AATTTNCCTAGAAGGCACAGTGCTGTTATTCCCACAATTTTGGGAGGCGAGGCAAGTGGG
  TCCACCCAGTCAAGAGATCGGGAACCTTCCTGCCCAATATGTGGAAACCCTGTCCTTCTA
  AAAATCCAAAAATTACCCGGCGGGTTGGCATGCCCCAATAT
  
• Restriction Sites: NotI-NotI
• ACCN: AK092450
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK092450.1
• RefSeq Size: 1738 bp
• RefSeq ORF: 1738 bp
• Locus ID: 6352
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway
• Gene Summary: This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]