POLR3A (AK024744) Human Untagged Clone

CAT#: SC102915

(untagged)-Human cDNA: FLJ21091 fis, clone CAS03642

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR3A
• Synonyms: ADDH; HLD7; hRPC155; RPC1; RPC155
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK024744, the custom clone sequence may differ by one or more nucleotides
  
  
  AATCCTTTACACCATGGTGAACCACGGCATGAGCATCGACAGGAGGCACGTGATGCTGCTCTCCGACCTC
  ATGACCTACAAGGGTGAAGTCCTGGGCATCACTAGGTTTGGCCTGGCCAAGATGAAGGAGAGTGTGCTGA
  TGCTGGCCTCCTTTGAGAAGACGGCTGACCATCTCTTTGACGCTGCCTACTTCGGGCAGAAGGACTCTGT
  GTGTGGGGTGTCTGAGTGCATCATCATGGGAATCCCAATGAACATTGGAACCGGGCTCTTCAAGCTGCTT
  CACAAGGCTGACAGGGACCCGAACCCTCCCAAGAGGCCCCTGATCTTCGACACAAATGAATTCCACATCC
  CCCTTGTCACATAGTCCAAAGAAAGAGGGGACCATGCCTGACCTTGACTCCTTGTCCTGTCTCCAGCTGA
  TGTATAAAGAGTTTTGTGCTCCCTGGGACGGGGGTCCTGAGGTCCCCACCTATGCCAGCAATCAGAGAAG
  CCCTCTTGGCATCCCCAGGAGCAGCTTCTCCTCTGATAGGGTGCAGCTCACACCAGTGACCCTGACTGTG
  CCACGCTGCTCGGGAGAGCTGAGGGTTTTATTGTTTGCTTGCTTGAAACCTAATCTATAGACGGCCCCAC
  AGCTCGTGCACACACTGCTTCCCTGGACTTAAAGCCCAGCCAGGTGTCATGGTCCATCCCAGCTCACTGC
  ATACATCCATCGGCTCCCCATGGTGTCTTCACACCTGACGATGAGCCAGGCCTGAGCCCCACACAGGCCA
  GGGCACATTCTTGGATTTTCCATTCCTTGGTCATGCTGGAATCTCTCAATGTGACATACTTATGTAAATA
  TTGTTACTATTATTTATTTGTTCCATTTGAGGGATTTGGAATTTTTGTTATTTTAGTTTTATTTTTGAAA
  CCAAGCATCTATAGAAACCAAGAAAGTCAGCATGTAAGCGTCACTGGAAAAACTGGTTTAAGCAAATAGA
  GCCGTCTGGGATTTGTAACTGAGGTGCAACTGTCATGAGGCCCAGGCAGCTCTGTAACATCTTCTATAGA
  TGCCCCTGGCTACCCTGTTGTTTTCATCTACCTCAGACCCCTATCATGGGGCTCTACCCTGTGACAAGAG
  CCAAACCCATTCTCCATGGCCTATGGAAGCCTCACTGGAGTTTGGGGCCTGCTGCAATGGGGATGAGATG
  GTTTTTTGTAGAATTATACTTACGTTCCTTGGATGATCTCTAGTTGATTTTTTAAGTTCTGAGTTGATGC
  TGTTAAGGTACCCGGGGTAGCCATTGGTTCTTGGATCTGTGTTAGAATGAGTGCTTTCCCTTCCTACTGA
  TGTGATTGTGGATTAGGAATTCGTGACCGAGTGATTTTTGGCCAGTGGTTGGGTTTAAAATTCTATTAAA
  ATTTGTAGTTTGGGCTGGGTGCTGTGGCTCATACCTATAATCCCAGCACTTTGGGAGGCCGAGGGGGGCA
  GATCACTTGAGCCCAGGAGTTCGAGAGCAGCCTGGGCAACATGACGAAACCCCGTCTCTACAGAGAATAC
  AAAAATTAGCCAGGCATGGTGGCCCATGCCTGTAGTACCTGCTATTGGTAAGGCTGAGGTGGAAGGTTCA
  CTTGAGCCTGGGAGGTGGAGGTTGTAGTGAGCCAAGATCGTGCCACTACACTCCAGCCTGGGCAACAAAG
  CAAGACCTTGTCTCAAAAAAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK024744 unedited
  TTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAAGAGCTCCAT
  GTACTACGTGCTGCAGTTCCTGAAAGAGGATCTCCCCAGGTGGTGGTGCAGGGCATTCCA
  GAGGTGTCCAGAGCTGTCATCCACATTGACGAGCAGAGTGGAAAGGAGAAGTACAAGCTT
  CTGGTGGAAGGTGATAACCTGCGGGCAGTCATGGCCACACACGGTGTGAAGGGCACCCGA
  ACCACCTCCAATAACACCTATGAGGTGGAGAAAACTCTGGGCATCGAGGCCGCCCGGACA
  ACGATCATCAATGAAATCCAGTACACCATGGTGAACCACGGCATGAGCATCGACAGGAGG
  CACGTGATGCTGCTCTCCGACCTCATGACCTACAAGGGTGAAGTCCTGGGCATCACTAGG
  TTTGGCCTGGCCAAGATGAAGGAGAGTGTGCTGATGCTGGCCTCCTTTGAGAAGACGGCT
  GACCATCTCTTTGACGCTGCCTACTTCGGGCAGAAGGACTCTGTGTGTGGGGTGTCTGAG
  TGCATCATCATGGGAATCCCAATGAACATTGGAACCGGGCTCTTCAAGCTGCTTCACAAG
  GCTGACAGGGACCCGAACCCCTCCAAGAGCCCCTGATCTTCGACACANATGAATCCACAT
  CCCCCTTGTCACATAGTCCAAAGAAAGAGGGACCATGCCTGACCTTGACTNCTTGTCCTG
  TCTCCAGCTGATGTATAAAAGATTTTTGTGCTCCCCTGGGACGGGGGGTCCTGAAGGTCC
  CCACCTATGCCCACCCATCAAAGAAGCCCTTCTTGGGAATCCCCAGAAGCAGTCTCTTCC
  TCTGGATAGTTGCAAGCTCACACCATTGACCTTGACTGGCCCCCCTGCTCGGGAAACCTG
  AGGGTTTTATATGGTGCTTGCTTGAACCCTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK024744 unedited
  GGTCATATTTATGNACCGCGGCACGCAATTTAGNGATCGAGTTTTTTTTTTTTTTTTTTG
  AGACAAGGTCTTGCTTTGTTGCCCAGGCTGGGGGGGAGTGGCACGATCTTGGCTCACTAC
  AACCTACACCTCCCAGGCTCAAGTGAACCTTCCACCTCAGCCTTACCAATAGCAGGTACT
  ACAGGCATGGGCCACCATGCCTGGCTAATTTTTGTATTCTCTGTATATACGGGGTTTCGT
  CATGTTGCCCAGGGGGGTCTCGAACTCCTGGGCTCAAGTGATCTGCCCCCCTCGGCCTCC
  CAAAGTGCTGGGATTATAGGTATGAGCCACAGCACCCAGCCCAAACTACAAATTTTAATA
  GAATTTTAAACCCAACCACTGGCCAAAAATCACTCGGTCACGAATTCCTAATCCACAATC
  ACATCAGTAGGAAGGGAAAGCACTCATTCTAACACAGATCCAAGAACCAATGGCTACCCC
  GGGTACCTTAACAGCATCAACTCAGAACTTAAAAAATCAACTAGAGATCATCCAAGGAAC
  GTAAGTATAATTCTACAAAAAACCATCTCATCCCCATTGCAGCAGGCCCCAAACTCCAGT
  GAGGCTTCCATAGGCCATGGAGAATGGGTTTGGCTCTTGTCACAGGGTAGAGCCCCATGA
  TAGGGGTCTGAGGTAGATGAAAACAACAGGGTAGCCAGGGGCATCTATAGAAGATGTTAC
  AGAGCTGCCTGGGCCTCATGACAGTTGCACCTCAGTTACAAATCCCAGACGGCTCTATTT
  GCTTAAACCAGTTTTTCCAGTGACGCTAACATGCTGACTTNTCTGGGTTCTATAGATGCT
  TGGTTTCAAAAATAAACTAAAATAACCAAATTTCCAATCCCTCAAATGGGACAAATTAAT
  TATAGTTACCATT
  
• Restriction Sites: NotI-NotI
• ACCN: AK024744
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK024744.1
• RefSeq Size: 1715 bp
• RefSeq ORF: 1715 bp
• Locus ID: 11128
• Cytogenetics: 10q22.3
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]