IL4I1 (NM_152899) Human Untagged Clone

CAT#: SC101062

IL4I1 (untagged)-Human interleukin 4 induced 1 (IL4I1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL4I1
• Synonyms: FIG1; hIL4I1; LAAO; LAO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101062 sequence for NM_152899 edited (data generated by NextGen Sequencing)
  ATGGCCCCATTGGCCCTGCACCTCCTCGTCCTCGTCCCCATCCTCCTCAGCCTGGTGGCC
  TCCCAGGACTGGAAGGCTGAACGCAGCCAAGACCCCTTCGAGAAATGCATGCAGGATCCT
  GACTATGAGCAGCTGCTCAAGGTGGTGACCTGGGGGCTCAATCGGACCCTGAAGCCCCAG
  AGGGTGATTGTGGTTGGCGCTGGTGTGGCCGGGCTGGTGGCCGCCAAGGTGCTCAGCGAT
  GCTGGACACAAGGTCACCATCCTGGAGGCAGATAACAGGATCGGGGGCCGCATCTTCACC
  TACCGGGACCAGAACACGGGCTGGATTGGGGAGCTGGGAGCCATGCGCATGCCCAGCTCT
  CACAGGATCCTCCACAAGCTCTGCCAGGGCCTGGGGCTCAACCTGACCAAGTTCACCCAG
  TACGACAAGAACACGTGGACGGAGGTGCACGAAGTGAAGCTGCGCAACTATGTGGTGGAG
  AAGGTGCCCGAGAAGCTGGGCTACGCCTTGCGTCCCCAGGAAAAGGGCCACTCGCCCGAA
  GACATCTACCAGATGGCTCTCAACCAGGCCCTCAAAGACCTCAAGGCACTGGGCTGCAGA
  AAGGCGATGAAGAAGTTTGAAAGGCACACGCTCTTGGAATATCTTCTCGGGGAGGGGAAC
  CTGAGCCGGCCGGCCGTGCAGCTTCTGGGAGACGTGATGTCCGAGGATGGCTTCTTCTAT
  CTCAGCTTCGCCGAGGCCCTCCGGGCCCACAGCTGCCTCAGCGACAGACTCCAGTACAGC
  CGCATCGTGGGTGGCTGGGACCTGCTGCCGCGCGCGCTGCTGAGCTCGCTGTCCGGGCTT
  GTGCTGTTGAACGCGCCCGTGGTGGCGATGACCCAGGGACCGCACGATGTGCACGTGCAG
  ATCGAGACCTCTCCCCCGGCGCGGAATCTGAAGGTGCTGAAGGCCGACGTGGTGCTGCTG
  ACGGCGAGCGGACCGGCGGTGAAGCGCATCACCTTCTCGCCGCCGCTGCCCCGCCACATG
  CAGGAGGCGCTGCGGAGGCTGCACTACGTGCCGGCCACCAAGGTGTTCCTAAGCTTCCGC
  AGGCCCTTCTGGCGCGAGGAGCACATTGAAGGCGGCCACTCAAACACCGATCGCCCGTCG
  CGCATGATTTTCTACCCGCCGCCGCGCGAGGGCGCGCTGCTGCTGGCCTCGTACACGTGG
  TCGGACGCGGCGGCAGCGTTCGCCGGCTTGAGCCGGGAAGAGGCGTTGCGCTTGGCGCTC
  GACGACGTGGCGGCATTGCACGGGCCTGTCGTGCGCCAGCTCTGGGACGGCACCGGCGTC
  GTCAAGCGTTGGGCGGAGGACCAGCACAGCCAGGGTGGCTTTGTGGTACAGCCGCCGGCG
  CTCTGGCAAACCGAAAAGGATGACTGGACGGTCCCTTATGGCCGCATCTACTTTGCCGGC
  GAGCACACCGCCTACCCGCACGGCTGGGTGGAGACGGCGGTCAAGTCGGCGCTGCGCGCC
  GCCATCAAGATCAACAGCCGGAAGGGGCCTGCATCGGACACGGCCAGCCCCGAGGGGCAC
  GCATCTGACATGGAGGGGCAGGGGCATGTGCATGGGGTGGCCAGCAGCCCCTCGCATGAC
  CTGGCAAAGGAAGAAGGCAGCCACCCTCCAGTCCAAGGCCAGTTATCTCTCCAAAACACG
  ACCCACACGAGGACCTCGCATTAA
  
  Clone variation with respect to NM_152899.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152899 unedited
  GATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCAGCCGCGCTGTCCT
  GCTGTCACCAAGAGCTGGAGACACCATCTCCCACCGAGAGTCATGGCCCCATTGGCCCTG
  CACCTCCTCGTCCTCGTCCCCATCCTCCTCAGCCTGGTGGCCTCCCAGGACTGGAAGGCT
  GAACGCAGCCAAGACCCCTTCGAGAAATGCATGCAGGATCCTGACTATGAGCAGCTGCTC
  AAGGTGGTGACCTGGGGGCTCAATCGGACCCTGAAGCCCCAGAGGGTGATTGTGGTTGGC
  GCTGGTGTGGCCGGGCTGGTGGCCGCCAAGGTGCTCAGCGATGCTGGACACAAGGTCACC
  ATCCTGGAGGCAGATAACAGGATCGGGGGCCGCATCTTCACCTACCGGGACCAGAACACG
  GGCTGGATTGGGGAGCTGGGAGCCATGCGCATGCCCAGCTCTCACAGGATCCTCCACAAG
  CTCTGCCAGGGCCTGGGGCTCAACCTGACCAAGTTCACCCAGTACGACAAGAACACGTGG
  ACGGAGGTGCACGAAGTGAAGCTGCGCAACTATGTGGTGGAGAAGGTGCCCGAGAAGCTG
  GGCTACGCCTTGCGTCCNCAGGAAAAGGGCCACTCGCCCCGAGACATCTACCAGATGGCT
  CTCAACCAGGCCCTCANAGACCTCAAGGCACTGGGCTGCAGAAAGGCGATGAAGAAGTTT
  GAAAGGCACACGCTCTTGGAATATCTTCTCGGGGAGGGGAACCNNTGAGCCGCCGCCGTG
  CAGCTTCTGGGAGACGTGATGTCCCGAGATGGCTTCNTCTATCTCAGCTTCGCCGAGGCC
  CTTCGGGNCCACAGCTGCCTCAGCGACAGACTCCAGTACAGCCGCATCGTGGGTGGCTGG
  GACCTGCTGCCGCGCGCGCTGCTGAGCTCGCTGTCCGGGCTTGTGCTGGTGGACGCGCCC
  CGTGTGGCGATGACCCAGGCCNGNACGATGTGCACGTGCAGATCGAGACTCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152899 unedited
  NNNAAATTCACTTGNACCGCGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAAAAAACTTTAATGCGAGGTCCT
  CGTGTGGGTCGGGTTTTGGAAAAATAACTGGCCTTGGACTGGAGGGGGGCTGCCTTCTTC
  CTTTGCCAGGTCATGCGAGGGGCTGCTGGCCACCCCATGCACATGCCCCTGCCCCTCCAT
  GTCAAATGCCTGCCCCTCGGGGCTGGCCGTGTCCAATGCAGGCCCCTTCCGGCTGTTGAT
  CTTGATGGGGGCCCCCAACGCCAACTTGACCGCCGTTTCCACCCAACCGTGCGGGTAGGC
  GGTGTGCTCCCCGGCAAAATAAATGCGGCCATAAGGGACCGCCCAGTCATCCTTTTCGGT
  TTGCCAAAACCCCGGCGGCTGTACCACAAAGCCACCCTGGCTGTGCTGGTCCTCCGCCCA
  ACGCTTGACAACCCCGGTGCCGTCCCAAAGCTGGCCCACGACAGGCCCGTGCAATGCCCC
  CACGTCGTCGAGCGCCAAGCGCAACGCCTTTTCCCGGCTAAAGCCGGCAAACGCTGCCGC
  CGCGTCCGACCACGTGTACAAGGCCAGCAGCAGCGCGCCCTCGCGCGGCGGCGGGTAAAA
  AATATGCCCCACGGGCGATCGGTGTTTGAGTGGCCCCCTTCAATGTGCTCCTCGCGCCAA
  AAGGGCCTGCGGAACCCTAAGGACCCCTTGGTGGGCCGCACCTAATGAAACCTCCGCAAC
  CCCTCCTCATGTTGGCGGGCCACCGCGCCAAAAAGTAAGCCCTTTACCGCGGTCCCTTCG
  CTTTAGAAACCCAGTTGGGCCTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152899
• Insert Size: 1875 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152899.1, NP_690863.1
• RefSeq Size: 1798 bp
• RefSeq ORF: 1704 bp
• Locus ID: 259307
• UniProt ID: Q96RQ9
• Cytogenetics: 19q13.33
• Domains: Amino_oxidase
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tryptophan metabolism, Tyrosine metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes a secreted L-amino acid oxidase protein which primarily catabolizes L-phenylalanine and, to a lesser extent, L-arginine. The expression of this gene is induced by the cytokine interleukin 4 in B cells. This gene is also expressed in macrophages and dendritic cells. This protein may play a role immune system escape as it is expressed in tumor-associated macrophages and suppresses T-cell responses. This protein also contains domains thought to be involved in the binding of flavin adenine dinucleotide (FAD) cofactor. Multiple transcript variants encoding different isoforms have been found for this gene. Some transcripts of this gene share a promoter and exons of the 5' UTR with the overlapping NUP62 gene. [provided by RefSeq, Jul 2020]
  Transcript Variant: This variant (1) differs in the 5' UTR and coding region compared to variant 2. The resulting isoform (1) contains a distinct and shorter N-terminus, as compared to isoform 2. This transcript contains the first two exons of the upstream and is expressed from a different promoter.