MARS2 (NM_138395) Human Untagged Clone

CAT#: SC100504

MARS2 (untagged)-Human methionyl-tRNA synthetase 2, mitochondrial (MARS2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARS2
• Synonyms: COXPD25; MetRS; mtMetRS
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100504 sequence for NM_138395 edited (data generated by NextGen Sequencing)
  ATGCTGCGAACGTCCGTCCTCCGCCTGCTAGGACGCACGGGGGCTAGTAGGCTGTCTCTC
  CTGGAGGACTTCGGCCCACGCTACTACAGTTCGGGCTCCCTCAGTGCCGGCGATGATGCT
  TGTGATGTGCGCGCCTACTTCACTACACCCATTTTCTACGTGAACGCGGCGCCGCACATC
  GGGCACCTGTACTCGGCACTACTGGCGGACGCCCTATGCCGCCACCGTCGCCTCCGAGGT
  CCCAGCACGGCCGCCACGCGATTCTCCACTGGTACCGACGAGCACGGGCTGAAGATTCAG
  CAGGCAGCAGCTACCGCGGGCCTGGCCCCGACCGAGCTGTGCGACCGAGTCTCTGAGCAG
  TTCCAGCAGCTTTTCCAGGAGGCCGGTATCTCCTGCACAGATTTCATCCGCACCACGGAG
  GCCCGGCACCGGGTGGCTGTGCAGCACTTCTGGGGGGTGCTTAAGTCCCGCGGTCTGCTC
  TACAAGGGCGTCTATGAAGGTTGGTATTGCGCTTCCGACGAGTGCTTCCTGCCTGAGGCC
  AAGGTCACCCAGCAGCCGGGCCCATCGGGGGATTCGTTTCCTGTATCTCTCGAGAGCGGG
  CATCCAGTCTCCTGGACCAAGGAAGAAAACTACATTTTCAGGCTTTCCCAGTTCCGGAAG
  CCACTCCAGCGGTGGCTGCGGGGCAACCCTCAGGCGATCACCCCCGAACCATTTCATCAC
  GTAGTTCTTCAGTGGCTGGACGAGGAGCTGCCCGACCTGTCCGTGTCTCGCAGAAGTAGC
  CACTTGCACTGGGGCATTCCGGTGCCCGGGGATGATTCGCAGACCATCTATGTATGGCTG
  GATGCCCTGGTCAACTACCTCACTGTAATTGGCTACCCAAATGCTGAGTTCAAATCTTGG
  TGGCCGGCCACCTCTCATATCATAGGTAAGGACATTCTCAAATTCCATGCCATCTATTGG
  CCTGCCTTCCTGTTAGGGGCCGGCATGAGCCCGCCACAGCGCATCTGTGTCCATTCCCAC
  TGGACAGTCTGTGGCCAAAAGATGTCCAAGAGCTTGGGCAACGTGGTGGATCCTAGGACT
  TGCCTTAACCGCTATACCGTGGATGGCTTCCGCTACTTTCTCCTTCGGCAGGGCGTCCCC
  AACTGGGACTGTGACTACTATGATGAAAAGGTGGTTAAGTTGCTGAACTCCGAGCTGGCA
  GATGCCTTGGGAGGTCTCTTGAACCGATGCACTGCCAAAAGAATAAATCCTTCTGAGACC
  TACCCAGCCTTCTGCACTACCTGCTTCCCTAGTGAGCCAGGGTTGGTGGGGCCGTCAGTT
  CGTGCTCAGGCAGAGGATTATGCTCTGGTGAGCGCAGTGGCCACTTTGCCAAAGCAGGTA
  GCAGACCACTATGATAACTTTCGGATATATAAGGCTCTGGAGGCCGTGTCCAGCTGTGTC
  CGGCAAACTAATGGTTTTGTCCAAAGGCATGCACCATGGAAGCTGAACTGGGAGAGCCCA
  GTGGATGCTCCCTGGCTGGGTACTGTGCTTCATGTGGCCTTGGAATGTTTGCGAGTCTTT
  GGGACTTTGCTGCAGCCTGTCACCCCAAGCCTAGCTGACAAGCTGCTGTCTAGGCTGGGG
  GTCTCTGCCTCAGAGAGGAGTCTTGGAGAGCTCTATTTCTTGCCTCGATTCTATGGACAT
  CCATGCCCTTTTGAAGGGAGGAGGCTGGGACCTGAAACTGGGCTTTTGTTTCCAAGACTA
  GACCAGTCCAGGACTTGGCTGGTGAAAGCCCACCGGACCTAG
  
  Clone variation with respect to NM_138395.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138395 unedited
  GCATTTCCCCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGGCACCATGCTGCGAACGTCCGTCCTCCGCCTGCTAGGACGCAC
  GGGGGCTAGTAGGCTGTCTCTCCTGGAGGACTTCGGCCCACGCTACTACAGTTCGGGCTC
  CCTCAGTGCCGGCGATGATGCTTGTGATGTGCGCGCCTACTTCACTACACCCATTTTCTA
  CGTGAACGCGGCGCCGCACATCGGGCACCTGTACTCGGCACTACTGGCGGACGCCCTATG
  CCGCCACCGTCGCCTCCGAGGTCCCAGCACGGCCGCCACGCGATTCTCCACTGGTACCGA
  CGAGCACGGGCTGAAGATTCAGCAGGCAGCAGCTACCGCGGGCCTGGCCCCGACCGAGCT
  GTGCGACCGAGTCTCTGAGCAGTTCCAGCAGCTTTTCCAGGAGGCCGGTATCTCCTGCAC
  AGATTTCATCCGCACCACGGAGGCCCGGCACCGGGTGGCTGTGCAGCACTTCTGGGGGGT
  GCTTAAGTCCCGCGGTCTGCTCTACAGGGCGTCTATGAAGGTTGGTATTGCGCTTCCGAC
  GAGTGCTTCCTGCCTGAGGCCAAGGTCACCCAGCAGCCGGGCCCATCGGNNGGATTCGTT
  TCCTGTATCTCTCGAGAGCGGGCATCCAGTCNTCTGGACCAAGGAAAGAAACTACATTNT
  CAGGCTNNTCCAGTTNCGGAAGCCACTNCAGCGGTGGGCTGCGGGCAACCCCTCAGCGAT
  CACCCNCGAACCATTTCATCACGTAGTNCTTTCAGTGGCTGGACGAAGAGCTGNCCGACC
  TGTCCGTGTCTCGCAGAAGTAGCCACTTGACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138395 unedited
  CGGAACTATCTATGGNACCGCGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTACCAACTTTTGAACTTTGATAACTTAAGTTAAAA
  GAGCAAAGGAAATAATCCGTTAAAATTACCTGAAAGTTGGGTATTAACATTTATAATAAC
  TTTTACACAATTTTTATATTGGATTAAAAATCCTGATTGATCTTATTAAAACACGAGGTA
  AATTATGCGGGGGGGGAACAAAATTAAAACTTTTAGAAATATAAAGAAAACATTGCCCTT
  TTGAACTATTTACAAATTTTTAAAACATACCTGGGACCCAATGTTACTAAAGTTTAAAAT
  TTTTAACATACCCAGGGCCTTAAGAGTTTTTTTATTTATTATAAAAACAACTTGGAACAT
  TTACCTATTCCCCTATTAAAACTTTCACAAAGTCAATGCCCTTCTCTAGCGATTCTAAAA
  AAAACCAGGCTTTTGGTAAACTTGGGGGGGGTTTTTGCTTTCCCTCAAAACAAAGGGGGT
  GGAAAGGTTTCCCCTTTTTTTAAAAAAACCCCAAAATGTTAAGACCTCCCAACTTTAGAA
  GCCAAAACCAATTTTTTCTCAACTTTCCAAACCTTTATCTTTTATAAGAAAACAGTGGGC
  GAAATAGAACCCTCTTCCCGGTGGAGGTGTGGGTGGCCCCACCCCCCCCTAGGGGGGCAT
  TCAACAAAACCGGGGGGAGCTTTTTGTGGGGAATATTAAATGGGGCGGACCCCATGGGGC
  AACAAAATACTTTTTCCACGCCACCGCGTGTTAAGGGGTCTAATAAAAACACTTCTTTTT
  TTTCGCCAAAACGACATGTTTGTTAAAAAAGGCGCCCCTCTAGAACAAAAAGGAGGGGGG
  GTGCCCCCCTAGCCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138395
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138395.2, NP_612404.1
• RefSeq Size: 3043 bp
• RefSeq ORF: 1782 bp
• Locus ID: 92935
• UniProt ID: Q96GW9
• Cytogenetics: 2q33.1
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Selenoamino acid metabolism
• Gene Summary: This gene produces a mitochondrial methionyl-tRNA synthetase protein that is encoded by the nuclear genome and imported to the mitochondrion. This protein likely functions as a monomer and is predicted to localize to the mitochondrial matrix. Mutations in this gene are associated with the autosomal recessive neurodegenerative disease spastic ataxia-3 (SPAX3). [provided by RefSeq, Apr 2014]