VARS2 (NM_020442) Human Untagged Clone

CAT#: SC100467

VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VARS2
• Synonyms: COXPD20; VALRS; VARS2L; VARSL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020442, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTCATTTGCCTCTCGCCTCTTTTCGACCACCATTTTGGGGGCTGAGGCACTCACGGGGCCTCCCCA
  GGTTTCACTCCGTTTCTACACAGTCGGAGCCCCATGGATCTCCCATCTCCCGGAGGAACCGTGAAGCCAA
  ACAGAAGCGCCTGCGAGAGAAGCAGGCGACTCTGGAGGCTGAGATAGCAGGGGAGAGCAAGTCACCTGCA
  GAATCCATTAAGGCCTGGAGGCCTAAGGAGTTAGTATTGTATGAAATCCCTACGAAACCCGGTGAAAAGA
  AAGATGTCTCTGGGCCCCTGCCTCCTGCATACAGCCCCCGATATGTTGAGGCTGCCTGGTACCCGTGGTG
  GGTACGAGAGGGCTTCTTCAAACCAGAATATCAGGCCCGGCTGCCCCAAGCTACAGGGGAGACCTTTTCC
  ATGTGTATCCCACCTCCCAATGTCACTGGCTCCCTGCACATTGGCCACGCACTCACGGTGGCCATACAGG
  ATGCCCTCGTGCGCTGGCACCGGATGCGTGGGGATCAAGTGCTGTGGGTCCCTGGTTCAGATCATGCAGG
  AATTGCTACACAAGCTGTGGTGGAGAAACAACTGTGGAAGGAACGGGGAGTGAGGAGACATGAGCTGAGC
  CGGGAGGCCTTCCTTAGGGAGGTGTGGCAGTGGAAGGAGGCGAAAGGTGGAGAGATCTGTGAGCAGCTGC
  GAGCTCTGGGTGCCTCCCTGGACTGGGATCGAGAGTGTTTTACCATGGATGTTGGCTCCTCAGTGGCTGT
  GACTGAAGCTTTTGTGCGGCTCTACAAGGCGGGGTTGCTGTACCGGAACCATCAGCTTGTCAACTGGTCA
  TGTGCTTTAAGATCAGCCATCTCGGACATTGAGGTGGAGAACCGGCCCCTGCCTGGCCACACACAGCTTC
  GACTGCCTGGCTGCCCCACCCCCGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTCCCCGTGGATGGAGA
  GCCTGATGCAGAGGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGATGTGGCTGTGGCCGTT
  CATCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCCTTGATGGGGCAGCCTC
  TTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGTGAAGGTGACTCCAGCTCA
  CAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAGCCCCTTGAATGTCATTGCGGAGGATGGG
  ACCATGACCTCCCTCTGCGGGGACTGGCTGCAGGGTCTTCACCGGTTTGTGGCCCGGGAAAAGATAATGT
  CTGTGCTGAGTGAATGGGGCCTGTTCCGGGGCCTCCAGAACCACCCCATGGTACTGCCCATCTGCAGCCG
  TTCTGGGGATGTGATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGCTGCCAGGAAATGGGGGCCCGA
  GCTGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTCCACCAGAAGAACTGGCAGCACT
  GGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCATCAGATTCCAGCCTACCT
  GGTTGTAGAGGACCATGCGCAGGGAGAAGAGGACTGTTGGGTGGTTGGGCGGTCAGAGGCTGAGGCCAGA
  GAGGTAGCAGCGGAACTGACAGGGAGGCCAGGGGCAGAGCTGACCCTGGAGAGGGATCCTGATGTCCTAG
  ACACATGGTTTTCTTCTGCCCTGTTCCCCTTTTCTGCCCTGGGCTGGCCCCAAGAGACCCCAGACCTTGC
  TCGTTTCTACCCCCTGTCACTTTTGGAAACGGGCAGCGACCTTCTGCTGTTCTGGGTGGGCCGCATGGTC
  ATGTTGGGGACCCAGCTCACAGGGCAGCTGCCCTTCAGCAAGGTGCTTCTTCATCCCATGGTTCGGGACA
  GGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAATGTGCTGGACCCAAGAGACATCATCAGTGGGGTGGA
  GATGCAGGTGCTGCAGGAAAAGCTGAGAAGCGGAAATTTGGACCCTGCAGAGCTGGCCATTGTGGCTGCA
  GCACAGAAAAAGGACTTTCCTCACGGGATCCCTGAGTGTGGGACAGATGCCCTGAGATTCACACTCTGCT
  CCCATGGAGTTCAGGCGGGCGACTTGCACCTGTCAGTCTCTGAGGTCCAGAGCTGCCGACATTTCTGCAA
  CAAGATCTGGAATGCTCTTCGCTTTATCCTCAATGCTTTAGGGGAGAAATTTGTGCCACAGCCTGCTGAG
  GAGCTGTCTCCCTCCTCCCCGATGGATGCCTGGATCCTGAGCCGCCTTGCCCTGGCTGCCCAGGAGTGTG
  AGCGGGGCTTCCTCACCCGAGAGCTCTCGCTCGTCACTCATGCCCTGCACCACTTCTGGCTTCACAACCT
  CTGTGACGTCTACCTGGAGGCTGTGAAGCCCGTGCTGTGGCACTCGCCCCGCCCCCTGGGGCCCCCTCAG
  GTCCTGTTCTCCTGCGCTGACCTCGGCCTCCGCCTCCTGGCCCCACTGATGCCCTTCCTGGCTGAAGAGC
  TCTGGCAGAGGCTGCCCCCCAGGCCTGGTTGCCCCCCTGCCCCCAGCATCTCGGTTGCCCCCTACCCCAG
  CGCCTGCAGCTTGGAGCACTGGCGCCAGCCAGAGCTGGAGCGGCGCTTCTCCCGGGTCCAAGAGGTCGTG
  CAGGTGCTAAGGGCTCTCCGAGCCACGTACCAGCTCACCAAAGCCCGGCCCCGAGTGCTGCTGCAGAGCT
  CAGAGCCTGGGGACCAGGGCCTCTTCGAGGCCTTCTTGGAGCCCCTGGGCACCCTGGGCTACTGTGGGGC
  TGTGGGCCTGTTACCCCCAGGCGCAGCAGCTCCCTCCGGCTGGGCCCAGGCTCCACTCAGTGACACGGCT
  CAAGTCTACATGGAGCTGCAGGGCCTGGTGGACCCGCAGATCCAGCTACCTCTGTTAGCCGCCCGAAGGT
  ACAAGTTGCAGAAGCAGCTTGACAGCCTCACAGCCAGGACCCCATCAGAAGGGGAGGCAGGGACTCAGAG
  GCAACAAAAGCTTTCTTCCCTCCAGCTGGAATTGTCAAAACTGGACAAGGCAGCCTCTCACCTCCGGCAG
  CTGATGGATGAGCCTCCAGCCCCAGGGAGCCCGGAGCTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020442 unedited
  GGTGTTCAAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACCCC
  CGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTCCCCGTGGATGGAGAGCCTGATGCAGA
  GGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGATGTGGCTGTGGCCGTTCA
  TCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCCTTGATGGG
  GCAGCCTCTTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGT
  GAAGGTGACTCCAGCTCACAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAG
  CCCCTTGAATGTCATTGCGGAGGATGGGACCATGACCTCTCTCTGCGGGGACTGGCTGCA
  GGGTCTTCACCGGTTTGTGGCCCGGGAAAAGATAATGTCTGTGCTGAGTGAACGGGGCCT
  GTTCCGGGGCCTCCAGAACCACCCCATGGTACTGCCCATCTGCAGCCGTTCTGGGGATGT
  GATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGCTGCCAGGAAATGGGGGCCCGAGC
  TGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTCCACCAGAAGAACTG
  GCAGCACTGGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCA
  TCAGATTCCAGCCTACCTGGTTGTAGAAGACCATGCGCAGGGAGAGAAGACTGTTGGGTG
  GTCGGGCGGTCAAAGGCTGATGCCANATAAGTANCANCNGAACTGACAGGGGAGCCAAGG
  GCANAGCTGACCCTGNAAAGGGATTCTGATGTNCTAAACACATTGTTTCTTCTGGCCTGT
  TTCCTTTTTTTGCCTGGGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020442 unedited
  TTCNANGATCGGATTTTTTTTTTTTTTTTTTGGGGATTCAAAATTTACTCATCTCCTCTT
  GGGGGACTTCCAGTGGGAGGCAAGCCGGGCAGTAATGTGAGCATCTCAAGGACACCACAG
  TCACTGCGACCAAGCCAGTCTGTGTCCTCATTTACACAATGAAGGCGATGGACCACATAG
  TCTCTGACGTCCCACTGCACCCTGACAGCTGACAAAGGGGGGGGTCCTCAAAAACAGGTC
  TGAGAGGGAGGAAAACTGATGGGGATGATGAGTTAAAGCTCCGGGCTCCCTGGGGCTGGA
  GGCTCATCCATCAACTGCCGGAGGTGAAAGGCTGCCTTGTCCAGTTTTGACAATTCCAGC
  TGGAGGGAAAAAAGCTTTTGTTGCCTCTGAGTCCCTGCCTCCCCTTCTGATGGGGTCCTG
  GCTGTGAGGCTATCAAGCTGCTTCTGCAACTTGTACCTTCGGGCGGCTAACAGAGGTAGC
  TGGATCTGCGGGTCCACCAGGCCCTGCAGCTCCATGTAGACTTGAGCCGTGTCACTGAGT
  GGAGCCTGGGCCCAGCCGGAGGGAGCTGCTGCGCCTGGGGGTAACAGGCCCACAGCCCCA
  CAGTAGCCCAGGGTGCCCAGGGGCTCCAAGAAGGCCTCGAAGAGGCCCTGGTCCCCAAGC
  TCTGAGCTCTGCAGCANCACTCGGGGCCGGGCTTTGGTGAGCTGGTACCTGGCTTGGAGA
  GCCCTTAGCACCTGGACGACCTCTTGGGACCCGGGAGAAGCGCCGCTCCCACCTCTGGCT
  GGCCCCCTGGCTCCAAGCTGGCAGCGCTGGGGTAGGGGGGAACCGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020442
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020442.2, NP_065175.2
• RefSeq Size: 3625 bp
• RefSeq ORF: 1506 bp
• Locus ID: 57176
• UniProt ID: Q5ST30
• Cytogenetics: 6p21.33
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (2) contains alternate 5' exon structure, and it thus differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1.