CDC26 (NM_139286) Human Untagged Clone

CAT#: SC100227

CDC26 (untagged)-Human cell division cycle 26 homolog (S. cerevisiae) (CDC26)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC26
• Synonyms: ANAPC12; APC12; C9orf17
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100227 sequence for NM_139286 edited (data generated by NextGen Sequencing)
  ATGCTGAGACGGAAACCAACACGCCTAGAGCTAAAGCTTGATGACATTGAAGAGTTTGAG
  AACATTCGAAAGGACCTGGAGACCCGTAAGAAACAGAAGGAAGATGTGGAAGTTGTAGGA
  GGCAGTGATGGAGAAGGAGCCATTGGGCTTAGCAGTGATCCCAAGAGCCGGGAACAAATG
  ATCAATGATCGGATTGGTTATAAACCCCAACCCAAGCCCAATAATCGTTCATCTCAATTT
  GGAAGTCTTGAATTTTAG
  
  Clone variation with respect to NM_139286.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139286 unedited
  NGGGGTCAACTTTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACCAGGCTAGGC
  CGGCTTGAAAAGAGATTTGACTGTACCTTTTAACTCTGTAGCTGGAACACAAGAAGTGTT
  TGTTTAATGAATGACGTACACATTTAAGATCTGTTTGGACGCGGAGGATAATCCTGTGAA
  TTGCTAATAGTTCACTGGGTTTGGCCCTTAGTGTTGACTTCAGTATGCTGAGACGGAAAC
  CAACACGCCTAGAGCTAAAGCTTGATGACATTGAAGAGTTTGAGAACATTCGAAAGGACC
  TGGAGACCCGTAAGAAACAGAAGGAAGATGTGGAAGTTGTAGGAGGCAGTGATGGAGAAG
  GAGCCATTGGGCTTAGCAGTGATCCCAAGAGCCGGGAACAAATGATCAATGATCGGATTG
  GTTATAAACCCCAACCCAAGCCCAATAATCGTTCATCTCAATTTGGAAGTCTTGAATTTT
  AGAGATGGATTATCTTGCATGCCAGAGCGCTGGAATGGAATAAAATGATGGCAGAAGTAC
  AAACCAGATTTAGAGAATTGAGTGCTTGCAGTCAAGCAGAATGTACCTCCTGCAGAGACA
  AATCTTCTGCATGAGATTACTGATGCTTCACTTGCACTCTAAGCTGGAATCCAAACTCTG
  GTTTGTCTCTTGAAAATTTGACTCTATAAAACTGATCTGATTTTCTGTTTTTAAAAATAA
  TATATTTTTGGAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATA
  GCTGTTTCCTGAACAGATCCCGGGTGGCATNCCTGTGACCCCTCCCAGTGCCTCTCCTGG
  CCCTGGAGTTGCCACTCCAGTGCCCACAGCCTTGTNNCTATAAAATAAGTGCATCATTTG
  TTGACTAGGTGTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139286 unedited
  NNNCCGGTAAACTAAGGAACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTT
  TTTTTCCAAAAAATATTTATTTTTAAAAACAGAAAATCGATCAGTTTTATAGAGTCAAAT
  TTTCAAGAGACAAACCAGAGTTTGGATTCCAGCTTAGAGTGCAAGTGAAGCATCAGTAAT
  CTCATGCAGAAGATTTGTCTCTGCAGGAGGTACATTCTGCTTGACTGCAAGCACTCAATT
  CTCTAAATCTGGTTTGTACTTCTGCCATCATTTTATTCCATTCCAGCGCTCTGGCATGCA
  AGATAATCCATCTCTAAAATTCAAGACTTCCAAATTGAGATGAACGATTATTGGGCTTGG
  GTTGGGGTTTATAACCAATCCGATCATTGATCATTTGTTCCCGGCTCTTGGGATCACTGC
  TAAGCCCAATGGCTCCTTCTCCATCACTGCCTCCTACAACTTCCACATCTTCCTTCTGTT
  TCTTACGGGTCTCCAGGTCCTTTCGAATGTTCTCAAACTCTTCAATGTCATCAAGCTTTA
  GCTCTAGGCGTGTTGGTTTCCGTCTCAGCATACTGAAGTCAACACTAAGGGCCAAACCCA
  GTGAACTATTAGCAATTCACAGGATTATCCTCCGCGTCCAAACAGATCTTAAATGTGTAC
  GTCATTCATTAAACAAACACTTCTTGTGTTCCAGCTACAGAGTTAAAAGGTACAGTCATA
  ATCTCTTTTCAAGCCGGCCTAGCCTGGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGT
  ATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACA
  CGCCTACCGCCCATTTGCGTCACGGGGGCGGGGTTATTACAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139286
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139286.3, NP_644815.1
• RefSeq Size: 885 bp
• RefSeq ORF: 258 bp
• Locus ID: 246184
• UniProt ID: Q8NHZ8
• Cytogenetics: 9q32
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc26, a component of cell cycle anaphase-promoting complex (APC). APC is composed of a group of highly conserved proteins and functions as a cell cycle-regulated ubiquitin-protein ligase. APC thus is responsible for the cell cycle regulated proteolysis of various proteins. [provided by RefSeq, Jul 2008]