GLUT4 (SLC2A4) (NM_001042) Human Tagged ORF Clone

CAT#: RG213717

SLC2A4 (tGFP-tagged) - Human solute carrier family 2 (facilitated glucose transporter), member 4 (SLC2A4)

Product Images

Circular map for RG213717

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GLUT4
• Synonyms: GLUT4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213717 representing NM_001042
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGAACCCCCTCAGCAGCGAGTGACTGGGACCC
  TGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTACAACATTGGGGTCATCAATGC
  CCCTCAGAAGGTGATTGAACAGAGCTACAATGAGACGTGGCTGGGGAGGCAGGGGCCTGAGGGACCCAGC
  TCCATCCCTCCAGGCACCCTCACCACCCTCTGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGA
  TTTCCTCCTTCCTCATTGGTATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGTCAACAATGT
  CCTGGCGGTGCTGGGGGGCAGCCTCATGGGCCTGGCCAACGCTGCTGCCTCCTATGAAATGCTCATCCTT
  GGACGATTCCTCATTGGCGCCTACTCAGGGCTGACATCAGGGCTGGTGCCCATGTACGTGGGGGAGATTG
  CTCCCACTCACCTGCGGGGCGCCCTGGGGACGCTCAACCAACTGGCCATTGTTATCGGCATTCTGATCGC
  CCAGGTGCTGGGCTTGGAGTCCCTCCTGGGCACTGCCAGCCTGTGGCCACTGCTCCTGGGCCTCACAGTG
  CTACCTGCCCTCCTGCAGCTGGTCCTGCTGCCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACATCATCC
  AGAATCTCGAGGGGCCTGCCAGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGATGTTTCTGGAGTGCT
  GGCTGAGCTGAAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCCACTGTCCCTGCTCCAGCTCCTGGGC
  AGCCGTACCCACCGGCAGCCCCTGATCATTGCGGTCGTGCTGCAGCTGAGCCAGCAGCTCTCTGGCATCA
  ATGCTGTTTTCTATTATTCGACCAGCATCTTCGAGACAGCAGGGGTAGGCCAGCCTGCCTATGCCACCAT
  AGGAGCTGGTGTGGTCAACACAGTCTTCACCTTGGTCTCGGTGTTGTTGGTGGAGCGGGCGGGGCGCCGG
  ACGCTCCATCTCCTGGGCCTGGCGGGCATGTGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCTCCTGC
  TGGAGCGAGTTCCAGCCATGAGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGTGGCATTTTTTGAGAT
  TGGCCCTGGCCCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAGCCAGGGACCCCGCCCGGCAGCCATG
  GCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTTCATCATTGGCATGGGTTTCCAGTATGTTGCGGAGG
  CTATGGGGCCCTACGTCTTCCTTCTATTTGCGGTCCTCCTGCTGGGCTTCTTCATCTTCACCTTCTTAAG
  AGTACCTGAAACTCGAGGCCGGACGTTTGACCAGATCTCAGCTGCCTTCCACCGGACACCCTCTCTTTTA
  GAGCAGGAGGTGAAACCCAGCACAGAACTTGAGTATTTAGGGCCAGATGAGAACGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213717 representing NM_001042
  Red=Cloning site Green=Tags(s)
  
  MPSGFQQIGSEDGEPPQQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNETWLGRQGPEGPS
  SIPPGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLVNNVLAVLGGSLMGLANAAASYEMLIL
  GRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILIAQVLGLESLLGTASLWPLLLGLTV
  LPALLQLVLLPFCPESPRYLYIIQNLEGPARKSLKRLTGWADVSGVLAELKDEKRKLERERPLSLLQLLG
  SRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFETAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRR
  TLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAM
  AVAGFSNWTSNFIIGMGFQYVAEAMGPYVFLLFAVLLLGFFIFTFLRVPETRGRTFDQISAAFHRTPSLL
  EQEVKPSTELEYLGPDEND
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042
• ORF Size: 1527 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042.1, NP_001033.1
• RefSeq Size: 2128 bp
• RefSeq ORF: 1530 bp
• Locus ID: 6517
• UniProt ID: P14672
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Type II diabetes mellitus
• Gene Summary: This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]