DRIL1 (ARID3A) (NM_005224) Human Tagged ORF Clone

CAT#: RC209254

ARID3A (Myc-DDK-tagged)-Human AT rich interactive domain 3A (BRIGHT-like) (ARID3A)

Product Images

Circular map for RC209254

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  Western blot validation of overexpression lysate (Cat# LY417431) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209254 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARID3A protein (Cat# TP309254). The protein was produced from HEK293T cells transfected with ARID3A cDNA clone (Cat# RC209254) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DRIL1
• Synonyms: BRIGHT; DRIL1; DRIL3; E2FBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209254 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTACAGGCCGTGATGGAGACGCTGTTGCAGCGGCAGCAGCGGGCGCGCCAGGAGCTGGAGGCCC
  GGCAGCAGCTGCCCCCCGATCCCCCTGCTGCACCCCACGGCCGGGCCCGGGCTGCCCCCGACGAGGACAG
  AGAGCCCGAAAGTGCCCGGATGCAGCGGGCTCAGATGGCCGCACTGGCAGCCATGCGGGCTGCAGCTGCG
  GGCCTGGGACACCCGGCCAGCCCCGGCGGCTCTGAGGATGGGCCCCCAGGCTCGGAGGAGGAGGACGCGG
  CCCGGGAGGGGACACCGGGCTCACCCGGGCGAGGCAGAGAAGGGCCAGGAGAGGAGCACTTTGAGGACAT
  GGCCTCCGACGAGGACATGAAGCCCAAATGGGAGGAGGAGGAGATGGAGGAAGACCTCGGGGAGGATGAG
  GAGGAGGAGGAGGAGGATTACGAGGATGAGGAGGAGGAGGAGGACGAGGAGGGGCTGGGCCCCCCAGGCC
  CTGCCAGCTTGGGCACCACGGCACTGTTCCCCCGAAAGGCCCAGCCACCCCAGGCCTTCCGCGGCGATGG
  CGTTCCCAGGGTGCTGGGGGGCCAGGAGCGGCCGGGGCCTGGCCCTGCCCACCCCGGAGGGGCCGCCCAC
  GTAGCCCCGCAGCTGCAGCCGCCTGACCACGGCGACTGGACTTACGAGGAGCAGTTTAAGCAGCTCTACG
  AACTCGACGGGGACCCCAAGAGGAAGGAATTCCTGGATGACTTGTTCAGCTTCATGCAGAAGCGAGGGAC
  ACCTGTGAACCGCATCCCCATCATGGCCAAACAGGTCCTTGACCTGTTCATGCTGTACGTGCTGGTGACG
  GAGAAGGGCGGCCTCGTGGAGGTCATCAACAAGAAGCTGTGGCGTGAGATCACCAAGGGCCTCAACCTGC
  CCACGTCCATCACCAGTGCAGCCTTCACCCTGCGGACCCAATACATGGAGTACCTGTACCCCTACGAGTG
  TGAGAAGCGGGGCCTCAGTAACCCCAATGAGCTCCAGGCAGCCATAGACAGCAACCGACGGGAGGGCCGG
  CGCCAGAGCTTTGGTGGCTCCCTCTTTGCCTACTCGCCAGGCGGGGCACACGGCATGCTCTCCTCACCCA
  AGCTACCCGTGTCCTCCCTGGGCCTGGCCGCAAGCACCAACGGCAGCTCCATCACCCCCGCCCCTAAGAT
  CAAGAAAGAGGAGGACTCAGCCATCCCCATCACAGTCCCTGGCCGCCTGCCTGTGTCCCTGGCGGGCCAC
  CCTGTGGTGGCAGCCCAGGCAGCAGCTGTGCAAGCAGCAGCCGCCCAAGCAGCTGTGGCTGCACAGGCAG
  CTGCCCTGGAACAGCTGCGGGAGAAGCTGGAGTCTGCGGAGCCTCCGGAGAAGAAGATGGCCCTGGTGGC
  CGATGAGCAGCAACGGCTGATGCAACGTGCACTCCAGCAGAACTTCCTGGCCATGGCGGCCCAGCTGCCC
  ATGAGCATTCGGATCAACAGCCAAGCCTCCGAAAGCCGCCAGGACTCTGCTGTGAACCTGACGGGCACCA
  ACGGCAGCAACAGCATCAGCATGTCGGTGGAGATCAACGGCATCATGTACACAGGAGTTCTGTTTGCTCA
  GCCGCCGGCCCCCACGCCAACCTCTGCTCCCAACAAAGGAGGCGGCGGCGGCGGCAGCAGCAGCAGCAAC
  GCAGGCGGCCGGGGAGGAAACACCGGAACCAGCGGCGGCCAGGCTGGGCCAGCGGGGCTGTCCACACCCT
  CCACATCTACCTCAAATAACTCGTTGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209254 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLQAVMETLLQRQQRARQELEARQQLPPDPPAAPHGRARAAPDEDREPESARMQRAQMAALAAMRAAAA
  GLGHPASPGGSEDGPPGSEEEDAAREGTPGSPGRGREGPGEEHFEDMASDEDMKPKWEEEEMEEDLGEDE
  EEEEEDYEDEEEEEDEEGLGPPGPASLGTTALFPRKAQPPQAFRGDGVPRVLGGQERPGPGPAHPGGAAH
  VAPQLQPPDHGDWTYEEQFKQLYELDGDPKRKEFLDDLFSFMQKRGTPVNRIPIMAKQVLDLFMLYVLVT
  EKGGLVEVINKKLWREITKGLNLPTSITSAAFTLRTQYMEYLYPYECEKRGLSNPNELQAAIDSNRREGR
  RQSFGGSLFAYSPGGAHGMLSSPKLPVSSLGLAASTNGSSITPAPKIKKEEDSAIPITVPGRLPVSLAGH
  PVVAAQAAAVQAAAAQAAVAAQAAALEQLREKLESAEPPEKKMALVADEQQRLMQRALQQNFLAMAAQLP
  MSIRINSQASESRQDSAVNLTGTNGSNSISMSVEINGIMYTGVLFAQPPAPTPTSAPNKGGGGGGSSSSN
  AGGRGGNTGTSGGQAGPAGLSTPSTSTSNNSLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005224
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005224.3
• RefSeq Size: 2823 bp
• RefSeq ORF: 1782 bp
• Locus ID: 1820
• UniProt ID: Q99856
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• MW: 63 kDa
• Gene Summary: This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008]