Chemokine Receptor D6 (ACKR2) (NM_001296) Human Tagged ORF Clone

CAT#: RC202459

CCBP2 (Myc-DDK-tagged)-Human chemokine binding protein 2 (CCBP2)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_001296" in other vectors (6)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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ACKR2 rabbit polyclonal antibody
    • 100 ul

USD 380.00

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol Chemokine Receptor D6
Synonyms CCBP2; CCR9; CCR10; CMKBR9; D6; hD6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202459 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCACTGCCTCTCCGCAGCCACTCGCCACTGAGGATGCCGATTCTGAGAATAGCAGCTTCTATT
ACTATGACTACCTGGATGAAGTGGCCTTCATGCTCTGCAGGAAGGATGCAGTGGTGTCCTTTGGCAAAGT
CTTCCTCCCAGTCTTCTATAGCCTGATTTTTGTGTTGGGCCTCAGCGGGAACCTCCTTCTTCTCATGGTC
TTGCTCCGTTACGTGCCTCGCAGGCGGATGGTTGAGATCTATCTGCTGAATCTGGCCATCTCCAACCTTC
TGTTTCTGGTGACACTGCCCTTCTGGGGCATCTCCGTGGCCTGGCATTGGGTCTTCGGGAGTTTCTTGTG
CAAGATGGTGAGCACTCTTTATACTATTAACTTTTACAGTGGCATCTTTTTCATTAGCTGCATGAGCCTG
GACAAGTACCTGGAGATCGTTCATGCTCAGCCCTACCACAGGCTGAGGACCCGGGCCAAGAGCCTGCTCC
TTGCTACCATAGTATGGGCTGTGTCCCTGGCCGTCTCCATCCCTGATATGGTCTTTGTACAGACACATGA
AAATCCCAAGGGTGTGTGGAACTGCCACGCAGATTTCGGCGGGCATGGGACCATTTGGAAGCTCTTCCTC
CGCTTCCAGCAGAACCTCCTAGGGTTTCTCCTTCCACTCCTTGCCATGATCTTCTTCTACTCCCGTATTG
GTTGTGTCTTGGTGAGGCTGAGGCCCGCAGGCCAGGGCCGGGCTTTAAAAATAGCTGCAGCCTTGGTGGT
GGCCTTCTTCGTGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGACCTGCAAGTA
TTCGGGAACTGTGAGGTCAGCCAGCATCTAGACTACGCACTCCAGGTAACAGAGAGCATCGCCTTCCTTC
ACTGCTGCTTTTCCCCCATCCTGTATGCCTTCTCCAGTCACCGCTTCCGCCAGTACCTGAAGGCTTTCCT
GGCTGCCGTGCTTGGATGGCACCTGGCACCTGGCACTGCCCAGGCCTCATTATCCAGCTGTTCTGAGAGC
AGCATACTTACTGCCCAAGAGGAAATGACTGGCATGAATGACCTTGGAGAGAGGCAGTCTGAGAACTACC
CTAACAAGGAGGATGTGGGGAATAAATCAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202459 protein sequence
Red=Cloning site Green=Tags(s)

MAATASPQPLATEDADSENSSFYYYDYLDEVAFMLCRKDAVVSFGKVFLPVFYSLIFVLGLSGNLLLLMV
LLRYVPRRRMVEIYLLNLAISNLLFLVTLPFWGISVAWHWVFGSFLCKMVSTLYTINFYSGIFFISCMSL
DKYLEIVHAQPYHRLRTRAKSLLLATIVWAVSLAVSIPDMVFVQTHENPKGVWNCHADFGGHGTIWKLFL
RFQQNLLGFLLPLLAMIFFYSRIGCVLVRLRPAGQGRALKIAAALVVAFFVLWFPYNLTLFLHTLLDLQV
FGNCEVSQHLDYALQVTESIAFLHCCFSPILYAFSSHRFRQYLKAFLAAVLGWHLAPGTAQASLSSCSES
SILTAQEEMTGMNDLGERQSENYPNKEDVGNKSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001296
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001296.5
RefSeq Size 2978 bp
RefSeq ORF 1155 bp
Locus ID 1238
UniProt ID O00590
Cytogenetics 3p22.1
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
MW 43.4 kDa
Gene Summary This gene encodes a beta chemokine receptor, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. [provided by RefSeq, Jul 2008]

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