MCHR (MCHR1) (NM_005297) Human Tagged ORF Clone

CAT#: RC200997

MCHR1 (Myc-DDK-tagged)-Human melanin-concentrating hormone receptor 1 (MCHR1)

Product Images

Circular map for RC200997

• 

  Western blot validation of overexpression lysate (Cat# LY417396) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200997 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MCHR
• Synonyms: GPR24; MCH-1R; MCH1R; SLC-1; SLC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200997 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGTGGGAGCCATGAAGAAGGGAGTGGGGAGGGCAGTTGGGCTTGGAGGCGGCAGCGGCTGCCAGG
  CTACGGAGGAAGACCCCCTTCCCGACTGCGGGGCTTGCGCTCCGGGACAAGGTGGCAGGCGCTGGAGGCT
  GCCGCAGCCTGCGTGGGTGGAGGGGAGCTCAGCTCGGTTGTGGGAGCAGGCGACCGGCACTGGCTGGATG
  GACCTGGAAGCCTCGCTGCTGCCCACTGGTCCCAATGCCAGCAACACCTCTGATGGCCCCGATAACCTCA
  CTTCGGCAGGATCACCTCCTCGCACGGGGAGCATCTCCTACATCAACATCATCATGCCTTCGGTGTTCGG
  CACCATCTGCCTCCTGGGCATCATCGGGAACTCCACGGTCATCTTCGCGGTCGTGAAGAAGTCCAAGCTG
  CACTGGTGCAACAACGTCCCCGACATCTTCATCATCAACCTCTCGGTAGTAGATCTCCTCTTTCTCCTGG
  GCATGCCCTTCATGATCCACCAGCTCATGGGCAATGGGGTGTGGCACTTTGGGGAGACCATGTGCACCCT
  CATCACGGCCATGGATGCCAATAGTCAGTTCACCAGCACCTACATCCTGACCGCCATGGCCATTGACCGC
  TACCTGGCCACTGTCCACCCCATCTCTTCCACGAAGTTCCGGAAGCCCTCTGTGGCCACCCTGGTGATCT
  GCCTCCTGTGGGCCCTCTCCTTCATCAGCATCACCCCTGTGTGGCTGTATGCCAGACTCATCCCCTTCCC
  AGGAGGTGCAGTGGGCTGCGGCATACGCCTGCCCAACCCAGACACTGACCTCTACTGGTTCACCCTGTAC
  CAGTTTTTCCTGGCCTTTGCCCTGCCTTTTGTGGTCATCACAGCCGCATACGTGAGGATCCTGCAGCGCA
  TGACGTCCTCAGTGGCCCCCGCCTCCCAGCGCAGCATCCGGCTGCGGACAAAGAGGGTGACCCGCACAGC
  CATCGCCATCTGTCTGGTCTTCTTTGTGTGCTGGGCACCCTACTATGTGCTACAGCTGACCCAGTTGTCC
  ATCAGCCGCCCGACCCTCACCTTTGTCTACTTATACAATGCGGCCATCAGCTTGGGCTATGCCAACAGCT
  GCCTCAACCCCTTTGTGTACATCGTGCTCTGTGAGACGTTCCGCAAACGCTTGGTCCTGTCGGTGAAGCC
  TGCAGCCCAGGGGCAGCTTCGCGCTGTCAGCAACGCTCAGACGGCTGACGAGGAGAGGACAGAAAGCAAA
  GGCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200997 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVGAMKKGVGRAVGLGGGSGCQATEEDPLPDCGACAPGQGGRRWRLPQPAWVEGSSARLWEQATGTGWM
  DLEASLLPTGPNASNTSDGPDNLTSAGSPPRTGSISYINIIMPSVFGTICLLGIIGNSTVIFAVVKKSKL
  HWCNNVPDIFIINLSVVDLLFLLGMPFMIHQLMGNGVWHFGETMCTLITAMDANSQFTSTYILTAMAIDR
  YLATVHPISSTKFRKPSVATLVICLLWALSFISITPVWLYARLIPFPGGAVGCGIRLPNPDTDLYWFTLY
  QFFLAFALPFVVITAAYVRILQRMTSSVAPASQRSIRLRTKRVTRTAIAICLVFFVCWAPYYVLQLTQLS
  ISRPTLTFVYLYNAAISLGYANSCLNPFVYIVLCETFRKRLVLSVKPAAQGQLRAVSNAQTADEERTESK
  GT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005297
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005297.2, NP_005288.2
• RefSeq Size: 2423 bp
• RefSeq ORF: 1062 bp
• Locus ID: 2847
• UniProt ID: Q99705
• Cytogenetics: 22q13.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 46 kDa
• Gene Summary: The protein encoded by this gene, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin. [provided by RefSeq, Jul 2008]