TMEM147 (NM_032635) Human Tagged ORF Clone

CAT#: RC200560

TMEM147 (Myc-DDK-tagged)-Human transmembrane protein 147 (TMEM147)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_032635" in other vectors (6)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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Rabbit Polyclonal antibody to Transmembrane protein 147 (transmembrane protein 147)
    • 100 ul

USD 533.00

Other products for "TMEM147"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TMEM147
Synonyms NIFIE14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200560 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGTTTCACTTCGGGAACTGCTTCGCTCTTGCCTACTTCCCCTACTTCATCACCTACAAGTGCA
GCGGCCTGTCCGAGTACAACGCCTTCTGGAAATGCGTCCAGGCTGGAGTCACCTACCTCTTTGTCCAACT
CTGCAAGATGCTGTTCTTGGCCACTTTCTTTCCCACCTGGGAAGGCGGCATCTATGACTTCATTGGGGAG
TTCATGAAGGCCAGCGTGGATGTGGCAGACCTGATAGGTCTAAACCTTGTCATGTCCCGGAATGCCGGCA
AGGGAGAGTACAAGATCATGGTTGCTGCCCTGGGCTGGGCCACTGCTGAGCTTATTATGTCCCGCTGCAT
TCCCCTATGGGTCGGAGCCCGGGGCATTGAGTTTGACTGGAAGTACATCCAGATGAGCATAGACTCCAAC
ATCAGTCTGGTCCATTACATCGTCGCGTCTGCTCAGGTCTGGATGATAACACGCTATGATCTGTACCACA
CCTTCCGGCCAGCTGTCCTCCTGCTGATGTTCCTCAGTGTCTACAAGGCCTTTGTTATGGAGACCTTCGT
CCACCTCTGCTCGCTGGGCAGTTGGGCAGCTCTACTGGCCCGAGCAGTGGTAACGGGGCTGCTGGCCCTC
AGCACTTTGGCCCTGTATGTCGCCGTTGTCAATGTGCACTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200560 protein sequence
Red=Cloning site Green=Tags(s)

MTLFHFGNCFALAYFPYFITYKCSGLSEYNAFWKCVQAGVTYLFVQLCKMLFLATFFPTWEGGIYDFIGE
FMKASVDVADLIGLNLVMSRNAGKGEYKIMVAALGWATAELIMSRCIPLWVGARGIEFDWKYIQMSIDSN
ISLVHYIVASAQVWMITRYDLYHTFRPAVLLLMFLSVYKAFVMETFVHLCSLGSWAALLARAVVTGLLAL
STLALYVAVVNVHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032635
ORF Size 672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032635.4
RefSeq Size 909 bp
RefSeq ORF 675 bp
Locus ID 10430
UniProt ID Q9BVK8
Cytogenetics 19q13.12
Protein Families Druggable Genome, GPCR, Transmembrane
MW 25.3 kDa
Gene Summary Acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (PubMed:21056967). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (PubMed:21056967).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.