SMURF 2 (SMURF2) Human Gene Knockout Kit (CRISPR)

CAT#: KN210866

SMURF2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: SMURF 2
• Locus ID: 64750
• Components:

  KN210866G1, SMURF 2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCCGTCAAGCTGCGCCTGAC

  KN210866G2, SMURF 2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGGCCCGTTCCTCCGGCCTC

  KN210866D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ACCCTCGGCG GCAGCTCACA CCCTCCCTGA CTGCCGGTGC CCGCCAGGGC ACCCTGCGCG CGCCCGCCGG CCCCGCCCCG GCCCCGCCCC GCCCCGCCGG CCCCGCCTCC CGTCTCCTCC CCGCCCCCCG GAGCCGACGA GTGGCGGCGG CGGCCCCAGG TCCCGGGTCC CTCAGACAAG AGGGCGGGTG GAGGAGGAAG CGGCCGAGCC CAGAGTTCTG CTCCGGCGCC CCCGAGCACC GCCCGCTTCA GCCGACCAGC CCCGTCGGCT ACTGGGCCTC GCCGAGACGA GAGGAGGGAA AGGCCTCGGC GGCCGCGAGG AGGCGGCGGG GGCGCGGGCG GAGGCGGCGC GGGCGGCCGC GGCTGCCGCT TTGTTGTGCG GCCCGGGCCG AGGAAGGAGA AGTGGGAGGA GGGGGGAGCT CGGCGTCCCG CTCCCTCCGC GGCTCATGGC GACGACTCTC GGCACATCCG GGACCCTCCG GCCGTGGCGG CCGAGGCGCC GGCTGCTCGG GCCCCAGCCC CGGCCGCTGT GGTGACTCCG CCGCGCCTCG CCGTCGCCCC CGTGCCGCCC GCCGCCCCCG CCGCCCCCGC CGCCGGGGAC GTGGGCGAGC TGGCGGGGGG TGCCTGTGGC CTTGGGAAGG GCCAGGGTCG CGATCAATGA TGAGTTGGGG GTCGGAGGAA TGGGAAGGCC TAGTTCAGGG AGAGTAATTG GAAAACCACT TGCTCTGGTT TGGACATTGT TTAAAAAGCC GAGGATGACT CGGGGCGGGG GGTGGTGTTT TGTGGAGGGG AGAATACACC GGTTCGCATC GCAGGCGGAT TTCAGGTCGG AAAGTCTTGG TCAAGGAGCC AGACGCTGCC TCCCGGGGCT CGGGGAACAG CCCCTGGGCT ACCGCCCAGG TGAGATCGTG AAGGGGATGA AGAGCTCCCG TGGCGAGGAT TATTGAATCC GGTTGCTGAC TGCGCGCAGT AAACACTCCT TAAAGATGGA GCGCTTAACG GGAGGGTGTG CGTGTGTGTT CCCGGGTTGT TACCTTGCAC ACACCCAGTT ATGCCCGCAT GAAAAACCTT TACTCCCCGC CGCCGGCCAC CGCTGCCCGG GCCACACACC TTAAACTCAT GTCTCTGAGG TGAGGAGACA GGTTGACTTT TCCGGTGTGC CTGTCTGATC CGACGGCTTG GGCTCATTGT ACACTGTGTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_022739
• UniProt ID: Q9HAU4
• Synonyms: DKFZp686F0270; MGC138150
• Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.[UniProtKB/Swiss-Prot Function]