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Home CRISPR-CAS9 CAS Vectors GE100025

pT7-Guide-IVT

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Specifications Citations (1) Validation Data FAQ
Cat. # Product Name Description Price Availability  
GE100025 pT7-Guide-IVT pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug) $330 In Stock Add to Shopping Cart
Features

T7 driven gRNA cloning vector

  • gRNA can be produced via in vitro transcription after the target sequence being cloned
  • BsmB I sites are used for target sequence cloning
  • For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014
  • M13 forward sequencing primer can be used to sequence the target sequence after cloning.

Download vector sequence

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